protein cleavage 中文意思是什麼

protein cleavage 解釋
蛋白質分解現象
  • protein : n. 【化學】朊，蛋白（質）。
  • cleavage : n. 1. 劈開，劈裂；劈開處。2. 【生物學】卵裂；分裂；【礦物】解理，劈理。
  1. Because of the cleavage site of enterokinase and cnbr was designed in the middle of thioredoxin and cmiv, the expressed peptides of the mutation of cmiv could be cuted down from the fusion protein by enterokinase or cnbr

    由於硫氧還蛋白和抗菌肽之間設計了腸激酶（ enterokinase ）切割位點和cnbr切割位點，通過對該表達的融合蛋白的切割，可得到目標抗菌肽cmiv突變體多肽分子。
  2. 2. the sequence of si gene from ibv - lx4 strain was consisted of 1614 bp from initiation codon atg to the possible cleavage site of spike glycoprotein, encoding for a 18 - amino signal - peptide with the n terminus of si protein and a polypeptide of 537 - amino acids. 19 highly conserved, potential glycosylation sites and 17 cysteines residues were characterized with si protein, homology analysis showe that there were gene deletion -

    S1基因：其全序列共1614bp （從起始密碼子atg到s前體蛋白裂解位點） ，編碼537個氨基酸，其氨基端有一編碼18個氨基酸的信號肽序列，第12 13位氨基酸殘基構成了信號肽的切割位點， 14 19位與111 124位氨基酸殘基為s1蛋白的跨膜區域。
  3. In vivo, the two functional forms of rat mapllc3 with apparent mobilities of 18 and 16kda, termed lc3 - i and lc3 - ii, separately, were produced by a series of post - translational modifications including a characteristic c - terminal cleavage after the conserved glyl20 residue and this cleavage is essential for the membrane association of the 16kda rat map1lc3 protein.,

    其中18kda的map1lc3蛋白是微管相關蛋白1的輕鏈亞基，稱為lc3 - ；另一種16kda的map1lc3蛋白則是自噬體膜的必須組分，稱為lc3 - 。 lc3 -和lc3 -都是一系列翻譯后修飾的產物，其中特徵性的修飾是在保守gly120后發生的羧基端切割，此切割修飾對lc3 -定位於自噬體膜尤為重要。
  4. In vivo, the two functional forms of rat map1lc3 with apparent mobilities of 18 and 16kb, termed lc3 - i and lc3 - ii, separately, were produced by a series of post - translational modifications including a characteristic c - terminal cleavage after the conserved gly120 residue and this cleavage was essential for the membrane association of the 16kd rat mapllc3 protein

    Lc3 ?和lc3 ?都是一系列翻譯后修飾的產物，其中特徵性的修飾是在保守gly120后發生的羧基端切割，此切割修飾對lc3 -定位於自噬體膜尤為重要。我們研究發現在大鼠，小鼠和人中map1lc3主要以兩種型存在， lc3a型和lc3b型。
  5. Ricin a chain ( rta ) is an active chain and has specific n - glycosidase activity, which excises a specific adenine residue ( a4324 in rat ) from a highly conserved loop of 26 or 28s rrna in 60s ribosomal subunits. this cleavage of adenine can lead to the disruption of ribosomal function, thereby, inhibits the protein synthesis and then cause the death of cells

    A鏈是活性鏈，具有n -糖苷酶活性，可催化切斷真核生物60s亞基28srrna中第4324位腺嘌呤與核糖分子之間的糖苷鍵，使其脫去一個腺嘌呤，使核糖體60s亞基失活，從而抑制蛋白質的合成。
  6. By using gfp - cam fusion protein, we have observed the detailed dynamic redistribution of cam in hela cells during cytokinesis. in tri - polar cell model, we have studied the relationship between the distribution pattern of cam and the formation of the cleavage furrow. furthermore, we have made the study on the regulation mechanism of cam during the whole process of cytokinesis by inhibiting its activity at different cytokinesis stages

    我們採用綠色熒光蛋白（ gfp ）標記技術，重點觀察了胞質分裂期hela細胞中gfp - cam的動態分佈；應用三極細胞模型研究了cam的分佈與分裂溝形成之間的關系；還通過在胞質分裂不同階段抑制cam的活性研究了cam在整個胞質分裂過程中的作用機制。
  7. This strain ' s virulence was judged by mean death time of chick embryos ( mdt ), intracerebral pathogenicity index in day - old chicks ( icpi ) and intravenous pathogenicity index in 6 - week - old chickens ( ivpi ) and it was found to be the virulent strain. at last, it was tested by the recurrent infection and found that it was the newcastle disease virus ( ndv ), and it was named hbg - 1 strain. in order to find the difference of the cleavage site of this strain with f48e9 and ? 30 strain, a part of the cleavage site of fusion protein gene fragment was amplified by rt - pcr using a primer and sequenced. the sequence analysis showed this strain had low homology with f4ge9 and cso. a phylogenetic tree based on the published sequences of ndv reference strains was constructed and showed the isolated strain hbg - 1 belonged to the genotype w ndv, a novel genotype ndv

    為了進一步探尋分離株與標準株的異同，又採用rt - pcr方法，擴增獲得分離株f _ o裂解位點附近的基因片段，經測序后與國際上已發表的新城疫病毒的核酸序列進行比較，結果表明其與標準株和疫苗株之間的同源性較低，僅為82 86之間。經系統發育進化樹分析后，判定該分離株為新城疫病毒（ ndv ）基因型。運用計算機軟體對其裂解位點處的氨基酸序列進行預測和分析，結果表明該分離株為新城疫病毒強毒株並具有基因型的典型結構特徵。
  8. The gene was 1668bp in length, encoding the f protein composed of 553 amino acids. sequence analysis and its secondary structure prediction showed that the f protein had three major hydrophobic regions consisting of about 25 amino acids, six potential glycosylation sites and thirteen cysteines. a sequence region of basic amino acids, rrqrrf, was found at the f, - f2 cleavage site, indicating that f4ge9 was a typical virulent strain

    序列分析和二級結構預測表明， f _ （ 48 ） e _ （ 9 ）株f蛋白含有3個分別由25個氨基酸組成的疏水區，存在6個潛在的糖基化位點， 13個半胱氨酸殘基，裂解位點區域的氨基酸序列為rrqrrf ，說明f _ （ 48 ） e _ 9株是一株典型的強毒株。
  9. The amino acids located between 560 - 600 are all hydrophobic, which were probably associated with the envelope membrane, there were 19 cysteines and 13 potential glycosylation sites in s2 protein, the cleavage recognitio

    與其他毒株間（除v18 91外）核昔酸序列的同源性為85 99 ，氨基酸序列的同源率為83 91 。
  10. Constructed the fusion protein expression vector of echistatin, . and identify it by dna sequencing. the vector can express echistatin fusion protein at a high level, the fusion protein molecular weight is about 16000 da in sds - page analysis, and the fusion protein consists more than 30 % of total protein, the fusion protein has specific antigen - antibody reaction with rabbit anti - echistatin polyclonal antibody. the fusion protein include : hpk5. his + 6tag, and echistatin. echistatin can be released by cnbr cleavage

    構建了echistatin融合蛋白表達載體，經dna測序鑒定正確后，溫控誘導表達，獲得了高效表達，表達產物經505一隊ge分析，分子量為16000oa ，符合預計的融合蛋白分子量，表達產物. ll 』菌體總蛋白的30 %以上， westem一blotting結果顯示，該日的蛋白能夠和echistatin多克隆抗體發生特異性抗原抗體反應，表明我們成功構建了eohistatin的高效融合表達克隆。
  11. Then after purification, renaturation, cleavage and ultrafiltration centrifugation, igf - 1 and des ( l - 3 ) igf - l protein were obtained

    Westernblot結果表明iptg誘導表達產物可與特異性igf - 1抗體發生反應。
  12. A reverse - transcriptase polymerase chain reaction ( rt - pcr ) based technique was developed to detect newcastle disease virus ( ndv ) of different poultry species origin. four oligonucleotide primers, based on the differences of nucleotide sequence at the cleavage site of fusion ( f ) protein gene between virulent and non - virulent strains of apmv - 1, were designed to amplify specific dna fragment from different viruses

    依據apmv - 1融合蛋白（ f ）基因裂解位點的核苷酸序列與其毒力的相關規律，分別設計合成了四條寡核苷酸引物，建立了一個可迅速檢測不同禽源apmv - 1並可鑒定強、弱毒株的逆轉錄酶?聚合酶鏈式反應（ rt - pcr ）技術。
  13. The cleavage site of putative signal peptide was predicted to occur between 28 ( ala ) and 29 ( ala ) thus the putative mature form of the protein composed of 92 amino acids with a molecular weight of 9. 2 kda

    推測的最可能的信號肽切割位點位於第28 （ alal和29 （ alal個氨基酸之間。椎測的成熟肽共有92個氨基酸，分子量9 zkda 。
  14. Then it was subjected to hydroxylamine cleavage to verify the predicted primary structure. result : gst / mpem fusion protein was expressed successfully in e. coli. we obtained certain amount of gst / mpem protein by means of affinity chromatography

    結果：在大腸桿菌中誘導表達出gst mpem融合蛋白，經過親和層析獲得一定量的gst mpem融合蛋白，它們具有預期正確的一級結構。
  15. In this study, we showed that the protein levels of the cyclin - dependent kinase inhibitor p21 rapidly increased in the one - cell staged fertilized eggs treated with pma. the cleavage from one - cell stage of the fertilized eggs into two - cell stage was inhibited

    但研究表明p21結合cdc2的能力不如其他cdks ，其介導的g2 m期的調控是由於p21蛋白抑制cdk2 cyclina復合物的活性，下調cdc25磷酸酶活性，阻止cdc2tyr - 15的去磷酸化，降低mpf活性。
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