protein glycosylation 中文意思是什麼

protein glycosylation 解釋
蛋白質糖基化
  1. 2. the sequence of si gene from ibv - lx4 strain was consisted of 1614 bp from initiation codon atg to the possible cleavage site of spike glycoprotein, encoding for a 18 - amino signal - peptide with the n terminus of si protein and a polypeptide of 537 - amino acids. 19 highly conserved, potential glycosylation sites and 17 cysteines residues were characterized with si protein, homology analysis showe that there were gene deletion -

    S1基因：其全序列共1614bp （從起始密碼子atg到s前體蛋白裂解位點） ，編碼537個氨基酸，其氨基端有一編碼18個氨基酸的信號肽序列，第12 13位氨基酸殘基構成了信號肽的切割位點， 14 19位與111 124位氨基酸殘基為s1蛋白的跨膜區域。
  2. The enzyme digest analysis shows that the arm repeats of c - terminal are conceivably conservative domain. in arc1 protein, there are some active sites including n - glycosylation sites, camp - and cgmp - dependent protein kinase phosphorylation sites, protein kinase c phosphorylation sites, casein kinase ii phosphorylation sites, tyrosine kinase phosphorylation sites, n - myristoylation sites, amidation sites and leucine zipper pattern. it probably take part in the signaling process of self - incompatibility

    同時在arc1蛋白質中還發現了拉鏈結構和多個磷酸化位點，包括camp和cgmp依賴的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨酸激酶磷酸化位點、糖基化位點等，拉鏈結構為arc1蛋白之間及與其它蛋白的相互作用提供了可能，而磷酸化位點是arc1參與信號傳導過程所必需的。
  3. The new synthesized protein was led to endoplastic reticulum cavity by eukaryotic secretory signal peptide sequence and then anchored to innerwall of endoplastic reticulum by kdel sequence, which interdicted the process of protein entering golgi body and cytoplasm, and then avoided heterogeneous glycosylation modification of foreign protein and prolonged the disappearance of half life of protein in organism. 2

    真核分泌信號肽序列可以引導新合成的蛋白質進入內質網腔， kdel序列將進入內質網腔的蛋白質錨定在內質網內壁上，從而阻斷了蛋白質進入高爾基體和細胞質的過程，進而避免了外源蛋白質的異源糖基化修飾，延長了蛋白質在生物體內的半衰期。
  4. Human gnt - v contains 741 amino acids with six potential sites for n - glycosylation and bears high homology to gnt - v of rat. its gene is located on chromosome 2q21 containing 17 exons. gnt - v protein is encoded by exons 2 - 17 as open reading frame

    人類gnt - v由741個氨基酸組成，有6個潛在的n -糖基化位點，基因定位於染色體2q21 ，含有17個外顯子，其開放閱讀框架由外顯子2 - 17進行編碼。
  5. Expression system renders products that are free of post - translational modifications, it is likely that the binding of crylab toxin to bt - r3 protein via protein - protein interaction and does not require glycosylation

    且該受體蛋白與cry1ab的結合部位位於bt - r3受體蛋白的胞外結構域。 bt毒蛋白與鈣粘蛋白受體的結合與受體是否糖基化無關。
  6. It is divided to extracellular and intracellular part by transmembrane domain. there are 13 n - glycosylation sites, 20 protein kinase c phosphorylation sites, 28 casein kinase ii phosphorylation sites, 4 tyrosine kinase phosphorylation sites and 15 n - myristoylation sites in the extracellular part of bt - r3 protein. an integrin recognition sequences rod lies in intracellular part of bt - r3 protein

    跨膜區域（ tmd ）將它分為胞內和胞外兩個部分，它的胞外有13個潛在的糖基化位點， 20個蛋白激酶c的磷酸化位點， 28個酪蛋白激酶的磷酸化位點， 4個酪氨酸酶的磷酸化位點， 15個豆蔻（十四烷基）酰化位點；它的胞內有1個整合蛋白（ integrin ）識別位點。
  7. The results showed that the open reading frame of chil - 15 cdna encompassed 564 base pairs ( bp ) and encoded a protein of 187 amino acids with three potential n - linked glycosylation sites, four conserved cysteine residues, two out - of - frame atg initiation codons in the 5 " untranslated region, and a signal peptide consisting of 66 amino acids. when it was compared with the published sequence of chil - 15 cdna, 7 mutant sites were found, and 5 amino acids were changed in predicted amino acids, which indicated that chil - 15 may be polymorphic

    結果顯示，本研究所用白來航雞il - 15cdna5 』非編碼區有兩個框外atg起始密碼子，開放閱讀框由564bp組成，編碼187個氨基酸，其中n末端信號肽含有66個氨基酸殘基，在第48 、 149和166位的天冬酰胺殘基上有三個潛在的n -糖基化位點。
  8. Huangyal4 was complete nucleotide sequence of 1 854 bp with a nucleotide orf ( 1575 bp ), which encoded a protein consisting of 524 aa with molecular weight of 62. 2 kda and pi of 8. 96. strongly basic ( + ) amino acids, strongly acidic ( - ) amino acids, hydrophobic amino acids and polar amino acids of the protein were 13. 74 %, 11. 64 %, 36. 45 % and 22. 70 % respectively, and predicted secondary structure of the protein revealed many conserved domains such as n - glycosylation site, protein kinase c phosphorylation site, casein kinase ii phosphorylation site, n - myristoylation site, camp - and cgmp - dependent protein kinase phosphorylation site, tyrosine kinase phosphorylation site and a cytochrome p450 cysteine heme - iron ligand signature which was typical of cytochrome p450. a - helix and b - sheet of the protein is 47. 7 %, 45. 0 % respectively

    Huangya14 ）為材料分離克隆到一個細胞色素p450基因，命名為bccyp86mf5 ， cdna全長1854bp ，含1575bp的完整開放閱讀框，編碼524個氨基酸，其編碼蛋白質的分子量為61 . 2kda 、等電點為8 . 96 ；堿性氨基酸、酸性氨基酸、疏水氨基酸和極性氨基酸分別占總氨基酸的13 . 74 、 11 . 64 、 36 . 45和22 . 70 ；二級結構預測包括n -糖基化位點、依賴于camp和cgmp的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨基酸激酶磷酸化位點、 n -豆蔻酰化位點和細胞色素p450的典型區域，半胱氨酸亞鐵血紅素配體信號區等， -螺旋和-折疊分別佔47 . 7 、 45 . 0 ；與bccyp86mf1基因的氨基酸序列同源性達到95 . 2 ，與擬南芥cyp86c4的達到85 . 9 。
  9. Hydrophobicity analysis predicted a 36 - residue hydrophobic signal peptide for secretion in the n - terminus, and no transmembrane region was present, suggesting it might be a type of secretory protein. some generic and atipical n - glycosylation sites were present in clsp, suggesting that the protein might represent a glycoprotein. the clsp protein contained one cub ( clr / cls, an embryonic sea urchin protein uegf, and bone morphogenetic protein i ) domain from 39th to 164th ammo acids, which is known to be involved in protein - protein or protein - substrate interaction, and a trypsin - like serine protease domain positioned from 244th to 483rd amino acids

    Clsp分子具有補體樣絲氨酸蛋白酶的多種結構特徵，包括36個氨基酸組成的疏水信號膚，一個cub （ clr / cls ， anembryonicseaurehinproteinuegf ， andbonemorphogeneticproteinl ）結構域和一個胰酶樣絲氨酸蛋白酶結構域（ t汀psin一likeserineproteasedomain ）和幾個保守的糖基化位點等，沒有發現有跨膜區的存在。
  10. The gene was 1668bp in length, encoding the f protein composed of 553 amino acids. sequence analysis and its secondary structure prediction showed that the f protein had three major hydrophobic regions consisting of about 25 amino acids, six potential glycosylation sites and thirteen cysteines. a sequence region of basic amino acids, rrqrrf, was found at the f, - f2 cleavage site, indicating that f4ge9 was a typical virulent strain

    序列分析和二級結構預測表明， f _ （ 48 ） e _ （ 9 ）株f蛋白含有3個分別由25個氨基酸組成的疏水區，存在6個潛在的糖基化位點， 13個半胱氨酸殘基，裂解位點區域的氨基酸序列為rrqrrf ，說明f _ （ 48 ） e _ 9株是一株典型的強毒株。
  11. Sequence analysis showed that the full length of this cdna which encodes 364 amino acids is 1398bp. it has 71 % and 69 % identities to lycopersicon esculentum and pisum sativum respectively in amino acid level. this gene is a membrane protein which has one signal peptide, seven transmembrane helices, three n - glycosylation sites and one o - glycosylation site

    序列分析表明，該基因的cdna全長為1398bp ，開放閱讀框為1095bp ，編碼一個364個氨基酸的多肽，與番茄和豌豆中的該家族基因分別具有71和69的氨基酸同源性，是一種膜蛋白，具有1個信號肽序列， 7個跨膜螺旋， 3個n ?糖基化位點和1個o ?糖基化位點，分子量大約為39 . 174kd ，等電點為8 . 07 。
  12. Protein glycosylation inhibitors

    蛋白質糖基化抑制劑
  13. Protein glycosylation, overview

    蛋白質糖基化
  14. Major difference of hn proteins lies in no. 18 - no. 75 amino acid residues on n - terminal which includes three active sites of hn protein. the influence on biological action, caused by the difference of hn protein, is needed to study further. hn gene has only four glycosylation sites, which is 1 or 2 less on amount than that of other strains. so, this difference can be take on as a natural mark of ndv b95 strain furthermore, the fragment encoding hn gene was excised from the positive clone pgem - hn with sail and saci enzyme, and purified by agrose gel fraction method. then the fragment was subcloned into the pet - 28c expressing vector digested by sail and saci restriction enzyme

    作者將正確的重組克隆質粒pgem - hn用saii 、 saci雙酶切，電泳回收目的片段，將其亞克隆到經saii 、 saci雙酶切處理的pet - 28c中，轉化大腸桿菌tgi感受態細胞，得到的轉化子經pcr鑒定和酶切分析，篩選出符合閱讀框的重組子，構建成重組表達質粒pet - hn ，並在大腸桿菌bl _ （ 21 ） （ de _ 3 ）宿主菌中成功地表達了含目的蛋白的融合蛋白，融合蛋白的分子量約66kd ，加入iptg誘導8h后，蛋白表達幾乎達到最高水平。
  15. The amino acids located between 560 - 600 are all hydrophobic, which were probably associated with the envelope membrane, there were 19 cysteines and 13 potential glycosylation sites in s2 protein, the cleavage recognitio

    與其他毒株間（除v18 91外）核昔酸序列的同源性為85 99 ，氨基酸序列的同源率為83 91 。
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