protein i 中文意思是什麼

protein i 解釋
蛋白i
  • protein : n. 【化學】朊，蛋白（質）。
  • i : pron （pl we ） 〈人稱代詞，第一人稱，單數，主格。 （poss adj my; obj me; poss pro mine ） 〉我。...
  1. I turn the protein in his dinner lamb chop into amino acids.

    我把他晚餐吃的羊排里的蛋白質轉變成氨基酸。
  2. The contents of this studies include : 1 ) according to the researches on the correlation between the function and structure of the cmiv from bombyx - moxi before by others, especially by lixinlal in naigin normal university of china, we have designed and sythesized the mutation i of the gene of cmiv that was different from the natural cmiv about 50 % in amino sequence, using the favorable condon of the ecoli. after cheked the result of synthesis by sequence, we have cloned the gene into 3 " of the gene of thioredoxin in the thio - fusion expression vector ( ptxfus ), and the fusion protein of thio - cmiv was highly expressed in soluble form

    本研究的內容包括：一、在前人對抗菌肽cmiv研究的基礎上，對n端和c端進行氨基酸保守變換，設計和合成了該基因，充分使用大腸桿菌偏愛的密碼子，並將該基因5端與硫氧還蛋白基因3端融合，通過ptxfus表達載體獲得較高可溶性表達（在15 sds - page膠上可見明顯的表達蛋白帶） 。
  3. The sds - page results showed the fusion protein was efficiently expressed in the soluble form. 3 ) the expressed fusion protein was purified and cleavaged by enterokinase to release the mutation i of cmiv and the mutation ii, whiches exhibited antibacterial activity to the ecoli. k12

    三、對以上表達的融合蛋白進行初步純化以及利用enterokinase切割融合蛋白，並進行抗菌活性檢測，結果表明所設計的cmiv突變體具有抗菌活性。
  4. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  5. In order to study the regulatory mechanism in gametogenesis of loxoblemmus doenitzi, the special expression of c - kit and c - myc is investigated by immunohistochemical method. the results show that there are kit positive protein granules on the cellular membrane of spermatomeres from metaphase i to anaphase ii, and in the head of mature spermatozoa not only in the testis but also in the spermatheca

    結果表明， kit在多伊棺頭蟋精子發生中期至後期的精母細胞膜上有陽性顆粒，在精巢和受精囊內精子頭部殘留的細胞質內也有陽性表達；但在出生后卵子發生過程中，發育中的卵母細胞上無陽性表達。
  6. Refreshment ? i ' ve got protein paste, carb laxative

    來點點心？我這里有蛋白質膏，碳水化合物補劑
  7. Refreshment ? i ' ve got protein paste, carb laxative.

    來點點心？我這里有蛋白質膏，碳水化合物補劑
  8. Sod2 from schizosaccharomyces pombe, which encodes a protein with homology to bacterial and mammalia na + / h + antiporters in plasma membrane and responses to efflux of na +, is an important gene i nvolving in homeostasis. though sod2 gene has been cloned, ectopic expression of sod2 in high plants has not been reported

    Sod2在裂殖酵母（ schizosaccharomycespombe ）中編碼位於質膜上的na ~ + h ~ +逆向轉運蛋白，控制na ~ +外排，對于保持裂殖酵母細胞的離子均衡非常重要。
  9. Two positive clones were sequenced, and the results showed that its nuclcotidc sequence includes an open reading segment which codes for a 45 - amino acids protein and three endonuclcase sites which arc1 bgii, bamh i and bgi ii, this protein was identified as metallothionein based on its characteristic described above and its similarity ( 85 % ) to the mtn gene of drosophila : the 10 cysteine residues present occur in five pairs of cys - x - cys, x is serine, valine, ilistidine or lysine

    結果顯示：擴增的cdna片段長度為289bp ，其中含有一個編碼45個氨基酸的開放閱讀框，閱讀框所編碼的氨基酸中含有10個半胱氨酸，且在序列中均排列成cys - x - cys ，其中x為ser 、 val 、 his或lys 。這些特徵說明擴增的基因片段為家蠅mt基因序列的一部分。此基因序列片段與果蠅mtn基因序列的同源性達到85 . 0 ，擴增的基因序列中含有三個內切酶位點bg 、 bam和bg ，這一點也和果蠅mtn基因十分相似。
  10. Measurement of hs - c - reactive protein, cardiac troponin i, creatine kinase isoenzyme mb and myoglobin in the diagnosis of acute coronary syndrome

    心肌標志物在不穩定型心絞痛和急性心肌梗死的臨界值
  11. However, kit protein doesn " t exist in oogenesis after birth. there are also c - myc positive protein granules in the spermatomeres from metaphase i to telophase ii, in the spermatozoa and in the secretory cells of the spermatheca. c - myc protein expresses not in the normal follicles, but in the follicular cells of the apoptotic ones

    Myc在處于減數分裂的中期至末期的精母細胞的細胞質或細胞核內呈陽性表達，在減數分裂后形成的精細胞和受精囊囊壁的上皮細胞的細胞質內也有陽性表達：但在出生后的卵子正常發生過程中無陽性表達，然而在凋亡卵泡的濾泡細胞的細胞質內有陽性表達。
  12. The protein - storing cells in swietenia macrophylla were found to be populus - type, i. e. ordinary parenchyma cells containing both vacuolar protein inclusions and starch grains

    用21kda蛋白質的抗血清進行兔疫印跡分析表明， 18kda蛋白質和21kda蛋白質有相同的抗原決定簇。
  13. These include phototropism, light - driven chloroplast movements and stomatal movements. the phot i gene of arabidopsis encodes an autophosphosphorylating protein kinase that functions as a photoreceptor for phototropism in response to low - intensity bl. up to now

    在高等植物中，藍光調控多個重要生理反應過程，其中包括下胚軸的向光反應、葉綠體在光下的重新分佈和氣孔的開放等。
  14. Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115. mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting

    本研究將含有n末端信號肽和c末端毒性區缺失的pap基因的表達載體ppic9k - p用sali酶切線性化后，通過電擊轉化整合p . pastorisgs115菌株細胞中。
  15. For each fedding treatment, the longitude and latitude of the root egg, the number of egg chambers with each developmental grade all changed in a parabola model. the results from native page indicated that there were two specific protein i. e. vitellogenin ( vg ) or vitellin ( vn ) in the female wasp of n. vitripennis

    同一種餵食處理中，卵巢長度的時間動態幾乎都無變化； 1級卵子數、 2級卵子數和成熟卵子數的變化，是拋物線變化趨勢，即先增加，然後減少；基部卵子短徑和長徑也是拋物線變化趨勢。
  16. After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s

    通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。
  17. In thl1 gene, there are some famililar enzyme digest sites including bamh i, hind, sac i and sal i. the sequence of thl1l from brassica oleracea l. shows 99 % identity to the sequence of thll from brassica napus l. there are some phosphorylation sites and an active site of thoiredoxin h members consisting of cppc in thll protein

    甘藍和油菜thl1基因序列有5個堿基的差異，而氨基酸僅有2個氨基酸的變異，同源性達99 。在thl1蛋白的氨基酸序列中發現了一個硫氧還蛋白家族的活性位點（ cppc ）和多個磷酸化位點。
  18. To search proteins that associate with the mouse mint protein and regulate notch signaling in nuclei, and to study the function and mechanism of mint - mediated transcription repression, yeast two - hybrid assay was used to screen proteins that interact with a fragment of mint ( f5, amino acids 2226 - 2959 ). from 4x106 yeast clones transformed with the bait plasmid and a cdna library of 9 dpc mouse embryo, fifty - one were positive for nutritional screening and p - galactosidase assay. restriction digestion identified 10 independent positive clones and these were analyzed by dna sequencing these clones represent 3 correctly fused cdna fragments, which are mint, alpha a - crystallin - binding protein i ( alphaa - crybpl ), and nuclear receptor co - repressor 1 ( n - corl ), respectively

    鑒于mwt基因編碼區較長，共有10799個堿基，故此我們將mint分為六段，分別命名為fi一f6 ，本研究以其中的f5 （ 222e959 ）和f6 （ 296s576 ）片段為研究對象，將h者分別插入pgb盯7載體中，結果顯示： mintfs可與核受體輔助抑制因子1階cori ） ， o晶狀體蛋白結合蛋白1hlphatcrybp入及mw加互作用，而f6可與igm的重鏈恆定區、泛素結合酶2l6和一個未知功能的新的蛋白基因進行結合。
  19. Hydrophobicity analysis predicted a 36 - residue hydrophobic signal peptide for secretion in the n - terminus, and no transmembrane region was present, suggesting it might be a type of secretory protein. some generic and atipical n - glycosylation sites were present in clsp, suggesting that the protein might represent a glycoprotein. the clsp protein contained one cub ( clr / cls, an embryonic sea urchin protein uegf, and bone morphogenetic protein i ) domain from 39th to 164th ammo acids, which is known to be involved in protein - protein or protein - substrate interaction, and a trypsin - like serine protease domain positioned from 244th to 483rd amino acids

    Clsp分子具有補體樣絲氨酸蛋白酶的多種結構特徵，包括36個氨基酸組成的疏水信號膚，一個cub （ clr / cls ， anembryonicseaurehinproteinuegf ， andbonemorphogeneticproteinl ）結構域和一個胰酶樣絲氨酸蛋白酶結構域（ t汀psin一likeserineproteasedomain ）和幾個保守的糖基化位點等，沒有發現有跨膜區的存在。
  20. Clr is a complement serine protease, composed of two cub ( clr / cls, an embryonic sea urchin protein uegf, and bone morphogenetic protein i ) domains, one egf domain and one serine protease domain

    補體組分c1r含有兩個cub結構域，一個egf結構域和一個絲氨酸蛋白酶結構域，是一種絲氨酸蛋白酶原。
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