protein localization 中文意思是什麼

protein localization 解釋
第八章 蛋白質定位
  • protein : n. 【化學】朊，蛋白（質）。
  • localization : n. 1. 定位，定域。2. 局限。3. 地方化。
  1. In order to get further evidence of the localization and distribution of emt - 1 in cells, we have prepared emt - 1 his6 - fusion protein and intend to abtain emt - 1 antibody for immuno - histochemistry assay

    為了進一步證實emt l在細胞內的定位及組織分佈，擬制備抗emt l抗體，進行免疫組化驗證。
  2. In our experiment, after light and dark adaptation, the retina of the macrobrachium rosenbergi was respective incubated in high calcium solution, physiological solution and low calcium solution. we studied the effect of calcium concentration on the content and subcellular localization of gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation by sds - page technology and imunoelectron microscopy technology. our study results indicated : 一 、 effects of calcium concentration on the soluble gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation

    而鈣離子對gq蛋白亞基活性有無影響還未見報道。我們以光適應和暗適應條件下的羅氏沼蝦復眼視網膜為材料，分別用高鈣溶液、生理溶液、低鈣溶液孵育后，通過sds ? page電泳技術及免疫膠體金電鏡技術，研究鈣離子濃度對光暗適應時羅氏沼蝦感光細胞gq蛋白亞基含量的影響及亞基亞細胞定位的影響。
  3. By sds - page and immuno - blotting, we found that a monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain antibody could react with cytoplasmic dynein intermediate chain - like protein at 67 kda in lily pollen. under confocal laser scanning microscopy after immunoflurescence labeling, we found that the dynein intermediate chain - like protein appeared punctated and was co - localization partly with microtubules in cytoplasm of lily pollen tube

    免疫熒光標記及激光共聚焦掃描顯微鏡觀察發現，類細胞質力蛋白中間鏈在百合花粉管中存在於顆粒狀細胞器上；免疫熒光雙標及激光共聚焦掃描顯微鏡觀察發現，百合花粉管中類細胞質力蛋白中間鏈和微管存在部分共分佈。
  4. The studies about prpc include its brain localization and putative physiological functions and the identification of its receptors ( binding candidates ). it ' s proved that prpc functions through binding with another protein

    目前己有的關于細胞型骯蛋白的研究包括蛋白在正常腦內的分介、生理功能以及對其受體（或結合蛋白）的確定。
  5. The localization and expression of prolactin receptor from inner mongolia alpas cashmere goat were studied by sacpic staining, in situ hybridization and western blotting. samples of skin were taken at interval three months from birth, three months old, six months old, nine months old, ten months old or twelve months old, which correspond to summer, autumn, winter and spring. paraffin sections of hair follicles were stained with sacpic staining and in situ hybridization. the protein of prolatin receptor is abstracted from samples of skin in order to study on expression of prolactin receptor. there are prolactin receptors in outer root sheath, dermal papilla and inner root sheath. the growth of primary follicle is continuous

    本實驗從絨山羊出生后每隔三個月采一次皮樣，共分為4個月齡（ 3 、 6 、 9 、 10或12 ）段，通過製作石蠟切片，原位雜交、染色，並提取皮樣蛋白做westernblotting等實驗研究方法，研究了催乳素受體mrna催乳素受體在不同生長季節的內蒙古阿爾巴斯白絨山羊皮膚毛囊中的定位與表達，染色結果發現阿爾巴斯白絨山羊初級毛囊全年持續生長，次級毛囊的生長情況隨季節而變化，秋冬季生長旺盛，夏季生長緩慢與絨毛生成規律呈正相關。
  6. We fused the gfp into the c - terminal region as well as n - terminal region of emt - 1 protein. the result showed that the localization of the protein encoded by the full length emt - 1 cdna was in endoplastic reticulum ( er ). extracellular region, transmembrane and intracellular region showed the similar cellular localization

    我們用綠色熒光蛋白融合於emt l全長及其不同截斷形式的梭基和氨基端，瞬時轉染cos 7細胞，通過熒光顯微鏡觀察，發現emt l編碼的蛋白呈現內質網定位的特點。
  7. An unusual rice calmodulin isoform, oscam61, was first obtained in our lab, which contains an n - terminal cam domain and a c - terminal basic extension with a potential prenylation site. in vitro activity assays confirm oscam61 as a functional calmodulin. using the green fluorescent protein ( gfp ) as a visual marker, we further studied subcellular localization of oscam61 in stably transformed tobacco cells

    利用綠色熒光蛋白（ greenfluorescentprotein ， gfp ）作為標記，研究了oscam61在煙草細胞中的定位， gfp - oscam61融合蛋白（具有開放的異戊烯化修飾位點）定位於細胞質膜和細胞器膜上，而oscam61 - gfp （異戊烯化修飾位點被gfp封閉）定位於細胞核的核質中。
  8. Green fluorescent protein is widely applied in researches of modern life science, such as gene product moved - process in vivo, protein localization, drug screening and preliminary selection of transgenic individual as a molecular marker

    綠色熒光蛋白這種獨特的生物學特性，使其作為報道基因在現代生命科學研究領域中，如細胞內基因產物的動態過程，蛋白質在細胞內的定位，藥物的篩選，以及轉基因個體的初步鑒定等等有著廣泛的應用。
  9. Green fluorescent protein has several good characters. under excitation of long uv light or blue light, it emits green fluorescence without requiring any exogenous substrates and cofactors. gfp gene expression can be used to monitor gene expression and protein localization in living cells and organisms. this is a development of revolutionary significance. the dna sequence of this gene can be re - engineered by mutagenesis and the gfp will get improved fluorescent properties. the applications of gfp will be wider and wider

    綠色熒光蛋白具有優良的特性，在藍光或長紫外光的激發下，不需要任何外源底物或內源輔助因子的參入就能發出綠色熒光.綠色熒光蛋白基因的表達可用來監控活細胞或生物體中基因表達和蛋白質的定位.這是一個革命性的進展.而且，對基因dna序列的改造有可能使綠色熒光蛋白的發光特性更加優良，從而其應用范圍會更加廣泛
  10. Bioinformatic analysis were performed to prepro - gynostemminl on the base composition, codon preference, amino acid composition, pi value, molecular weight, secondary structure, prosite scan, prediction of protein localization sites. blast thtf arabidopsis thaliana genome, and 3d structure modeling

    運用生物信息學軟體對絞股藍班p前體進行堿基組成和密碼子偏倚性、氨基酸組成特徵、功能位點、亞細胞定位、跨膜結構、核酸結合基序以及空間結構等方面的分析。
  11. 三 、 effects of calcium concentration on subcellular localization of gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation. effects of calcium concentration on subcellular localization of gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation were studied using immunogold electronmicroscopy

    三、鈣離子濃度對光暗適應羅氏沼蝦感光細胞gq蛋白q亞基亞細胞定位的影響採用免疫膠體金電鏡技術，研究光暗適應條件下不同鈣離子濃度對羅氏沼蝦感光細胞中gq蛋白。
  12. The cellular localization of hse was done by using in situ hybridization, the results showed that primary spermtatocytes and spermatids in seminiferous tubular have positive signals. the subcellular localizations of hsei and hseii were identified by gfp fusion protein. hsei - gfp fusion protein was evenly distributing in cytoplasm, while hseii - gfp was not evenly distributing in cytoplasm and there were many bright spots in cytosol

    用gfp高合蛋白的技術確定hsei和hsell在細胞中勺定位，結果顯示hsei gfp均勻地分佈於細胞漿中，而hsell0fp則分佈不均勻，在胞漿有聚集成許多熒光信號很強的點。
  13. A novel reporter gene, green fluorescent protein gene ( gfp ) has been widely used in study on gene expression and protein localization among living organisms

    主要研究結果如下： 1考查了廣宿主范圍穩定性質粒ptr102應用於華癸中生根瘤菌分子生物學研究的可行性。
  14. To make clear the hypothesis, a middle cerebral artery occlusion ( mcao ) and hypoxia and glucose - deprivation ( hgd ) ischemic models were used in in vivo and in vitro study, respectively. we first studied the cellular localization of kvl. 2 and the co - localization of kvl. 2 protein and vegf receptors flk - 1 and flt - 1, observed the effect of mcao on kvl. 2 expression and phosphrylation in the rat brain in vivo, then investigated the effect of vegf on ischemia / hypoxia cell damage and tyrosine phosphorylation of kvl. 2 in sh - sy5y cells. finally, in order to further elucidate the relationship between vegf ' s neuroprotection and its regulation on kvl. 2 phosphorylation, we used a specific antisense oligodeoxynucleotide ( odn ) to knockdown the expression of endogenous vegf to observe its role in ischemia / hypoxia cell damage and regulation of kvl. 2 phosphorylation

    為了驗證上述假設，本文分別在整體和離體水平，採用大腦中動脈缺血（ middlecerebralarteryocclusion ， mcao ）和體外氧?糖剝奪（ hypoxiaandglucose - deprivation ， hgd ）缺血模型，首先了解了kv1 . 2蛋白的細胞定位及與vegf受體flk - 1和flt - 1的共存情況，觀察了整體mcao后缺血再灌不同時間大鼠腦內kv1 . 2蛋白的磷酸化水平變化，然後通過外源性給予vegf蛋白，在sh - sy5y細胞株上觀察其對缺血細胞存活率及kv1 . 2蛋白磷酸化水平的影響，最後利用vegf反義脫氧寡核苷酸（ oligodeoxynucleotide ， odn ）特異阻斷內源性vegf蛋白的表達，觀察內源性vegf蛋白在缺血細胞損傷及調節kv1 . 2蛋白磷酸化中的作用，以進一步明確vegf缺血保護效應與其調節kv1 . 2蛋白磷酸化之間的關系。
  15. Identified as encoding protein that bind msx2, mint, human gene termed as sharp ( smrt / hdac1 associated represser protein ), is a large nascent polypeptide of 3576 amino acids and has three n - terminal rna recognition motifs ( rrms ) and four nuclear localization signals

    Mint屬於一個新的spen （ splitends ）蛋白家族成員。 spen是果蠅中一個分子量為600kda的巨大核蛋白，參與果蠅發育過程中神經元蟬的決定和神經元突起延伸的調節。
  16. This article mainly discusses the challenges, research approaches and recent developed tools in the field of protein function prediction and the ways by which these issues change the process of drug discovery, including homology - based annotation transfer, sequence motifs and patterns, information on 3d structure, sub - cellular localization, posttranslational modifications, binding sites and functional residues, protein - protein interactions

    摘要簡述了在蛋白質功能預測領域中的研究方法和最新研發工具所面臨的挑戰，並討論蛋白質功能預測是如何改變藥物開發進展的，具體包括：基於序列同源性分析的注釋轉移、序列基元和模式增加了注釋轉移的說服力、 3d結構信息可以精煉注釋轉移、亞細胞定位、翻譯后修飾、結合位點和功能殘基、蛋白質之間的相互作用。
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