prv 中文意思是什麼

prv 解釋
蛋白質置換值
  1. Experiment shows that the antigen extracted by autoclave is best for dot - ppa - elisa. applying the x2 test, optimal concentration of antigen was determined, and the optimum dilution of enzyme hrp - spa was 1 : 10. the high specificity of dot - ppa - elisa was proved by the specific blocking test, and also by the cross - reaction test in which the diaphragm does not react with the antibodies against salmonellosis, pasteurellosis, chlamydiosis, hcv, ppv, brucellosis, erysipelas suis, colibacillosis, prv

    試驗中對多種方法提取的診斷抗原進行了比較、篩選；確定了dot - ppa - elisa的最佳工作條件；測定了st - 171株的免疫豬血清抗體及c群、 2型人工感染豬血清抗體效價，並初步確定了該方法的陽性標準。
  2. The high specificity of the dot - ppa - elisa was confirmed by specific blocking test and also by cross - reaction test. the diaphragm did not react with the antibodies against salmonellosis, streptococcosis, colibacillosis, chlamydiosis, hcv, ppv, prv, brucellosis. erysipelas, suis and chlamydiosis in cross - reaction test. the diaphragm has good sensitivity and could detect some pasteurella - positive test serum which has been diluted to2 - "

    試驗證明所建立的dot - ppa - elisa具有較好的特異性，與豬瘟、仔豬副傷寒、豬丹毒、豬細小病毒病、豬偽狂犬、豬布氏桿菌病、豬衣原體病陽性血清無交叉反應。
  3. Porcine rotavirus ( prv ) belongs to the family reoviridae. it is one of the major pathogens of diarrhea in piglets and causes a major health worldwide in graziery. infection of prv is also prevalent in china, in some area the infectious ratio of piglets is 72 %

    豬輪狀病毒（ porcinerotavirus ， prv ）是呼腸孤病毒科輪狀病毒屬的成員，是引起仔豬病毒性腹瀉的主要病原之一，給世界各地的畜牧業造成嚴重的經濟損失。
  4. Pseudorabies virus ( prv ) is the causative agent of aujeszky ' s disease, which results in significant losses in pig husbandry. thymidine kinase ( tk ) gene is one of the main virulent genes of prv, and it is essential to the propagation of prv in the nerve tissues, but dispensable for virus replication and infection in other tissues

    偽狂犬病是由偽狂犬病病毒（ pseudorabiesvirus ， prv ）引起的家畜和多種野生動物的一種以發熱、奇癢、呼吸和神經系統疾病為特徵的急性傳染病，妊娠母豬可發生死胎和流產，是危害養豬業的一個重要傳染病。
  5. Accurate diagnosis and effectiv veccination are main means for the prevention of porcine diarrha researh showed tha the outer capsid glycoprotein vp7 of prv particle is major protective anigen

    病毒外膜的vp7糖蛋白是rv的主要免疫保護性抗原，由其刺激機體產生的抗體對中和病毒、消除感染起主要作用。
  6. This study provides the basis evidence and material basis for prv identification molecular epdiemiology investigation, research of diagnostic reagent and genetic engineering vaccine

    本研究為國內prv毒株鑒定、分子流行病學調查、分子診斷試劑的開發以及基因工程疫苗的研製提供了理論依據和物質基礎。
  7. This study provides scientific theoretical information for the molecular epidemiology of pseudorabies in guangxi and lays a good foundation for constructing ge gene delected vaccine and the diagnostic method of identifying prv

    這些結果對我區偽狂犬病病毒的分子流行病學調查和構建ge基因缺失疫苗，建立偽狂犬病的鑒別診斷方法奠定了基礎。
  8. Western - blot analysis showed the expressed protein was specific to antisera against prv fa strain. the ge - elisa for differentiation of prv infected from vaccination was developed using the expressed ge protein as antigen

    Coli中高效表達的偽狂犬病病毒ge蛋白為抗原，以辣根過氧化物酶（ hrp ）標記的spa為二抗，建立了ge - elisa鑒別診斷方法。
  9. The transfer vector pbdtk - uni can be used for the construction of recombinant prv expressing foreign gene ( s ). postgraduate : tianzhijun specialty : preventive veterinary science supervisors : prof. li yijing prof. tong guangzhi

    以上結果表明所構建的具有自主知識產權的通用prv轉移載體pbdtk - uni是成功的，為利用該載體構建偽狂犬病病毒二價基因工程疫苗提供了技術平臺。
  10. Pseudorabies, c aused b y p seudorabies v irus ( prv ), i s a d isease c haracterized b y neurological disorder in piglets and reproductive failure in pregnant pigs. the vaccines currently used in china and other countries are all with ge - phenotype

    偽狂犬病病毒（ pseudorabiesvirus ， prv ）歸屬于皰疹病毒亞科，主要引起豬的偽狂犬病（ pr ） ，該病以仔豬的神經學癥狀和妊娠母豬的生殖障礙為主要特徵。
  11. It revealed a negative reaction with positive sera of prv hcv, prrsv, jev and sa215. it revealed a positive reaction with prv standand positive serum. the results showed that the established ge - elisa has the advantages shch as high sensibility strong specificity and good repeatability and can be used to differentiation of prv infection from vaccination

    與豬細小病毒、豬瘟病毒、豬乙型腦炎病毒、 prrsv的標準陽性血清呈陰性反應，與偽狂犬病病毒標準陽性血清呈陽性反應，與三基因缺失疫苗株sa215免疫豬所采血清呈陰性反應。
  12. A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a, yielding a 1. 7kb band. the segment was linked to puc19 plasma dna by means of t4 dna ligase, transformed into e. coli jm109 permissive cells, and incubated on lb fray containg amp, x - gal and iptg. small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr, resulting in recombinant plasma puge dna containing prv ge

    用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接；用連接產物轉化大腸桿菌jml09感受態細胞，置含amp 、 x - gal和iptg的lb平板上培養12 20小時；挑取白色菌落於選擇性培養基擴大培養，堿裂解法小量提取質粒dna ，並進行酶切分析鑒定，結果獲得整合有prvge基因的重組質粒pugedna ，並與其它prv分離株進行ge基因序列同源性分析。
  13. The high specificity of dot - ppa - elisa was proved by the specific blocking test, and also by the cross - reaction test in which the diaphragm did n ' t react with the antibodies against pasteurellosis, streptococcosis, colibacillosis, chlamydiosis, hcv, ppv, brucellosis, prv and foot - mouth disease. the diaphragm has good sensitivity and could detect some salmonella - positive test serum which has been diluted to 1 : 2048. stored at 4 for at least 6 months or at 10 - 25 " c for 4 months, the sensitivity and specif icity of the diaphragm did n ' t change, so it has good stability

    本研究制備的診斷膜片特異性強：不與豬衣原體病、豬口蹄疫病、豬大腸桿菌病、豬布氏桿菌病、豬瘟、豬偽狂犬病、豬細小病毒病、豬巴氏桿菌病、豬鏈球菌病的陽性血清發生交叉反應；診斷膜片具有良好的敏感性，能夠檢測到1 ： 2048稀釋的動物試驗陽性血清；膜片的保存期長，在10 25可保存4個月、 4條件下至少可保存6個月其靈敏度不變。
  14. To differentiate the animals immunized with rprv - ha and those naturally infected with prv or sfv in future, two differentiating diagnostic methods were developed based on recombinant viral antigens

    為了區分此重組病毒疫苗免疫的動物和自然感染prv和siv的動物，本項研究利用基因工程抗原建立了兩種分子鑒別診斷方法。
  15. The purpose of this study is to construct a recombinant pseudorabies virus expressing ha gene of swine influenza virus and to develop specific diagnosis for differentiating the recombinant virus immunized animals and those naturally infected with prv or sfv

    本試驗的目的是以ge基因缺失的prv為載體構建可以表達sivha基因的重組病毒疫苗用於預防這兩種病，同時，基於此重組病毒的特點，建立可以區分此重組病毒疫苗和prv及siv自然感染的鑒別診斷方法。
  16. Finally, a tranfer vector named as pltk - ha was constructed based on pltk - uni with insertion of ha gene of h3 subtype srv. the pltk - ha and prv bartha - k61 genomic dna were co - transfected into 50 - 70 % confluent vero cells in 6 - cm dishes. based on the expression of lacz gene, recombinant prv were selected and purified by blue - colored virus plaque

    利用脂質體介導的方法，將pltk - ha與prvbartha - k61基因組共轉染于亞單層vero細胞，依據報告基因lacz在細胞中的表達，篩選藍色蝕斑的重組病毒，經數次蝕斑純化后， pcr鑒定、 western - blot分析。
  17. Accurate diagnosis and effective vaccination are main means for the prevention of prv diarrhea

    我國prv感染也非常普遍，有些地區斷奶仔豬陽性率高達72 。
  18. Porcine rotavirus ( prv ) belongs to the family reovindae. it is one of the major pathogens that cause life threatening diarrhea in piglets. this kind of diadrrhea was the easiest infected by young animal

    豬輪狀病毒（ porcinerotavims ， prv ）屬弧腸孤病毒科輪狀病毒屬成員，是引起仔豬病毒性腹瀉的主要病原之一。
  19. After obvious cytopathogenic effects developed, virus - contained supematants were harvested, and the progeny viruses were screened for lacz - expressing viruses by a plaque assay using x - gal. single blue plaques were picked, and a recombinant prv stably expressing lacz gene ( designated as rprv - lacz ) was obtained after ten cycles of plague purification and pcr identification. the results showed that the lacz gene expression cassette was stably expressed in the recombinant rprv - lacz derived from bartha - k61 strain

    該載體與具有高度感染性的bartha - k61株基因組dna通過脂質體加plus法共轉染vero細胞，採用甲基纖維素固定病變， x - gal染色，經過10代藍斑純化獲得了一株穩定表達lacz基因的ge tk基因缺失突變株，命名為rprv - lacz 。
  20. A - l was infected on monolayer sffi ther the third plaque purification to reproduce in quantity and named it a - 2. after a - 2 was infectal on sffi monolayer cell 4d harvested the cpe cell. the analysis results with sds - page and dot - elisa showed that prv vp7 gene was - - o - - abstract expressed in baculovirus

    接種a - 2代病毒于sf9單層細胞上， 4d后收獲病變細胞，通過sds -聚丙烯酰胺凝膠電泳（ sds - page ）和免疫酶斑點技術（ dot - elisa ）分析，表明prv - vp7基因在桿狀病毒表達系統中得到了表達。
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