pstvd 中文意思是什麼
pstvd
解釋
馬鈴薯紡錘形塊莖類病毒-
To device a primer pairs and amplify the full length pstvd by rt - pcr, the positive rna extraction from tuber sample was used as template. the product of rt - pcr was purified and connected to the plasmid pmd 18 - t vector
Cdna核酸斑點雜交反應（ nash ）檢測pstvd方法準確、靈敏度高，一次檢測樣品數量多，且對于異地樣品檢測非常方便，是以往其它檢測方法的有效補充。 -
Then, the plasmid was transformed into jm109. the full length pstvd cdna recombinant plasmid was further identified by restriction mapping analysis and the nucleotide sequence of cdna cloned in pmd 18 - t vector was analyzed with ab1 377 dna sequence. test showed that the cdna of this chinese pstvd isolate was the same as pstvd - kf - 6 mild " type " isolate which originated from naturally infected potatoes in field of cornell university potato breeding program
利用rt - pgr技術，設計一對引物，對田間採集的經鑒定含pstvd的陽性樣品進行全序列擴增，並將所得產物純化回收，連接到pmd18t - vector中，並轉化至大腸桿菌jm109中，挑選白斑進行雙酶切（ saci / ecorv ）鑒定證明插入片段為359bp大小，進行序列測定，所得克隆基因為pstvd全序列負鏈，大小為359bp 。 -
It can be futher verified by the test result that the pstvd found in the northeast region of china may be introduced into china by following the introduction of the potato cultivar irish cobbler to various countries and irish cobbler was imported in heilongjiang province prior to the 1960s
在國內，首次對中國分離株系進行cdna基因克隆和序列分析，進一步驗證了中國株系的由來及其結構特點，對于中國在pstvd檢測水平的提高、檢測試劑盒制備、 pstvd的防治及其致病機理、轉基因育種等提供重要依據。 -
All the samples with typical potato spindle tuber viroid ( pstvd ) symptoms collected from the fields in the northeast region of china were detected by the r - page method. the positive samples were verified. then, two - dimensional page and rt - pcr techniques were adapted in deleting pstvd
對采自中國東北佳木斯、大慶等地區具有典型馬鈴薯紡錘塊莖類病毒（ pstvd ）癥狀的薯塊樣品進行往返電泳（ r - page ） ，確定含馬鈴薯紡錘塊莖類病毒的陽性樣品。
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