purified product 中文意思是什麼
purified product
解釋
精製產品-
According to these problems, we adopt to the method of mending material, optimize to fermentation media and partly ferment condition. finally, we excogitate a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified. with the plasmid pbv220 - ifnr, pbv220 - hgfa, pbv220 - hgfb, pbv220 - hpk5 that expresses serve as the model, adopting the biostat - c15l of b. braun company, utilize the method of mending material to ferment, through optimization fermentation media and optimization partly ferment condition ( ventilate quantity, stir speed, mend material speed ), eventually establishment a kind of fermentation technology that is suitable for target gene efficiency expressed and is advantageous of product purified
以我室構建並穩定表達的重組質粒pbv220 - - ifn 、 pbv220 - hgf 、 pbv220 - hgf 、 pbv220 - hpk5為模型，分別從不同的表達宿主菌中篩選出一種適合大規模生產的菌種bl21 （ de3 ） ，該工程菌株連續傳代100代表達質粒不丟失，表達量穩定；採用b . braun公司的biostat - c15l自控發酵罐，運用分批補料技術分別進行四種工程菌的高密度發酵，通過優化工程菌發酵的培養基配方及優化部分發酵條件（通氣量、攪拌速度、補料速度） ，最終建立一種適于目的基因高效表達的高密度發酵工藝模式。 -
The 600 bp and 800 bp pcr products were cloned into the pgem - t easy vector. their cdna sequences were determined with positive clones or purified pcr product. conclusion : compared the 600 bp pcr product with the amino acids sequence for the fibrinolysin metalloproteinase from the venom of agkistrodon acutus from the southern of anhui province, their homology is 90. 6 %
結果：其中一對引物擴增得到一600bp產物；另一對引物擴增得到三條特異性的dna條帶，大小分別為1 . 5kb 、 1 . 3kb和800bp ，將600bp和800bpdna進行克隆及測序，並推導編碼的氨基酸序列。 -
Porcine transmissible gastroenteristis is an importan contagious disease endangering the development of swine. in other to establish a rapid diagnosis method and provide effective immunogenic products, the nucleoprotein ( n ) gene of porcine transmissible gastroenteristis virus ( tgev ) was cloned. expressed and its expressed product was purified
為建立對豬傳染性胃腸炎快速有效的診斷方法，並試圖在預防上提供有效的免疫制劑，本論文首次在我國對豬傳染性胃腸炎病毒核衣殼蛋白基因進行了克隆、鑒定、表達及重組核蛋白的純化；並在細胞上對重組核衣殼蛋白抗體的中和效力進行了測定。 -
Then the pcr product was purified, ligated into pgem - t vector by ta cloning
篩選陽性克隆，測序，大量制備序列完全正確的質粒。 -
The expressed product was lysised with supersonic wave and purified by sds - page. elisa analysis revealed that the antigenicity of the vpi protein has been detected. the forth part - detection of aev - nh937 strain by in situ hybridization ( 1sh ) - probe was labelled with digoxigenin ( dig ). then the probe hybrid with 5d, 10d, and 20d postinfection brain tissue of chicken. the results of the ish showed that the positive signal was found in 3 cases, while control group was negative. there has been a reasonable correlatien between this method and other detection test
經超聲波裂解，用尿素溶解包涵體，電泳純化后，利用elisa檢測vp _ 1外殼蛋白，表明具有一定抗原性；第四部分：應用原位雜交檢測aevi用dig標記的探針與sd 、 10d 、 20d的攻毒雞腦組織雜交，來檢測那v的rna 。 -
Is precisely inunder this kind of high boundary management pursue power inspiration, the dream silk family member in for several years productionmanagement, always persisted strengthens " the pure silk real quality " quality consciousness, the firm rejection 絲綿 or under the footmaterial takes the bed by the short fiber, persisted with the longtextile fiber pure silk, the purified cotton cotton material, theenvironmental protection cotton material makes the raw material, andin the national same business the one ' s own issue " in the market likediscovered a dream silk product has the adulteration raw materialcompensates 10, 000 yuan " the serious pledge
正是在這種高境界的經營追求動力鼓舞下，夢絲家人在幾年來的生產經營中，始終堅持強化「真絲真品質」的質量意識，堅決拒絕以短纖維絲綿或下腳料作為填充物，堅持用長纖維純蠶絲、純棉布料、環保布料作原料，並在全國同業內獨家發布「市場上如發現一件夢絲家產品有摻假原料賠償一萬元」的鄭重承諾。 -
After one of them was expressed in e. coli bl21, the product was purified by ni - nta column
經酶切鑒定證實原核表達載體ptat爿ascfv構建成功。 -
At the same time an apparatus of zone melting is designed, the method of zone melting is used to purify the purified product of recrystalizing. after purification, the homogeneity and stability of the purified product of antioxidant d and bht are examined by high performance liquid chromatography ( hplc )
實驗研究結果：防老劑d重結晶提純工藝為：每次取10g工業品防老劑d ，經過堿洗后，去離子水洗滌至中性，溶於100ml乙醇中，同時用粉狀活性炭（ 1g次）脫色、過濾、母液靜置2h后，再放入冰箱結晶2h 。 -
Its products mainly cover 9 key series : air filter, air purification equipment, purifying energy - saving & environment - friendly air conditioner, air cleaner, anti - static product, air control valve, filter cotton, purified water filtration core and filter
公司生產和經營的產品主要分為九大系列：空氣過濾器；空氣凈化設備；凈化型節能環保空調；空氣凈化機；防靜電；風口調節閥；過濾棉；純水濾芯；濾清器。 -
The product is isolated and purified from natural fresh tomatoes
番茄紅素是無環類胡蘿卜素，分子式為 -
The special high - purified graphite is one kind of product specially processed which is characterized in low mo and fe to be the superior materials for mercury - free alkali high - energy battery
特種高純石墨是一種經特殊加工工藝處理后的產品，其特點是低鉬、低鐵，是生產綠色無汞堿性一次高能電池的優質原料。 -
To device a primer pairs and amplify the full length pstvd by rt - pcr, the positive rna extraction from tuber sample was used as template. the product of rt - pcr was purified and connected to the plasmid pmd 18 - t vector
Cdna核酸斑點雜交反應（ nash ）檢測pstvd方法準確、靈敏度高，一次檢測樣品數量多，且對于異地樣品檢測非常方便，是以往其它檢測方法的有效補充。 -
The bioactive strain ' s fermentation product was isolated and purified primarily using methods of solvent extraction, acid - alkali extraction, ab - 8 macropore absorption chromatography, 1 x 007 positive ion exchange chromatography, the result showed the purification product has prominent bioactivity inhibit staphalococcus aureus
中國熱帶農業科學院、華南熱帶農業大學2003屆碩士研究生採用有機劑抽提法、酸堿抽提法、大孔吸附樹脂柱層析和陽離子交換樹脂柱層析等方法對hsl 306菌株發酵產物進行了初步分離純化。 -
Then the product was purified by glutathione sepharose 4b chelation affinity chromatography, and upper purified gst - gnrh / trs fusion proteins was received for further the foundations established in the scientific research and the real application
表達產物經谷胱甘肽瓊脂糖4b親和層析純化后，得到了純度較高的gst - gnrh trs融合蛋白。為進一步科研和實際應用奠定基礎。 -
After washing with reagent, adopt the newest purification technology source30rpc, sds - page and densitometric scan analysis, the result show that expression level is 90 % of total bacterial proteins. after renaturation, ifnr, hgfa, hgfb, hpk5 were purified by akta purifier chromatogram instrument, sepharose fast flow, ssphacrayl series gel, selecting optimize condition. finally establish a kind of high efficient purification model of recombinant proteins produced in escherichia coli as inclusion bodies, purification product purity > 98 %
結論:總之，通過對發酵罐中重組工程菌各種培養因素的研究，建立了一種高密度、高表達發酵工藝體系，為重組蛋白的后續純化提供了大量、穩定的原料供應;通過對不同目的蛋白的色譜行為的系統研究，建立了一種高效穩定、快速簡潔、易於放大的包涵體重組蛋白分離純化體系。 -
First, the purified pezzis and pcr product of angiostatin are digested by ecor. i and xba i. after purifying the digested products respectively, we ligate these two kinds of dna by t4 dna ligase and construct the recombinant plasmid pezz18 - as. then transform it to the competent e. coli dh5a
用限制性內切酶ecori與xbai對目的基因as 、表達載體pezz18行雙酶切，酶切產物純化后利用大腸桿菌t _ 4dna連接酶連接構成重組子pezz18 - as ，並轉化e . colidh5 ，經氨芐青霉素lb平板初篩后，以菌液pcr和重組子的單、雙酶切行進一步鑒定。 -
Molecular weight of the purified product determined by high performance gel filter chromatography was 60634d, which was approximately equivalent to that of biostim ( 55036d )
結果必思添的保留時間為13 . 5分鐘，分子質量55036d ；本文提取的kp莢膜糖蛋白保留時間為12 . 86分鐘，分子質量為60634d 。 -
The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2, ha order to prepare ndrg2 antibody, the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector. after induced by iptg, the fusion proteins were expressed in e. coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization, rabbits produced high titer antiserum against ndrg2. then antisemm was absorbed using ndrg2 antigen immobilized on nc filters, the purified product of antiserum shows high special to ndrg2 protein, and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach
為制備高效價的ndrgz抗體，分別構建了prset a雌、 pgex4t d唾倉和pgex4tl七三種原核重組表達質粒，並在大腸桿菌中誘導表達出相應的融合蛋白；用全長gstjqdrgz蛋白免疫兔，然後用gst ndrgz人和gstjqdrgze片段加強免疫，經免疫得到了較高效價的兔抗人ndrz多克隆抗血清，利用固定於硝酸纖維素膜上的ndrgz抗原親和吸附純化抗血清，提高了ndrgz抗體的特異性；並對包涵體形式表達的6his ndrgz進行初步的分離純化。 -
The activity of the purified product was confirmed by indirect elisa analysis and was further confirmed by indirect immunofluorescence and immunohistochemistry after they were added to the culture medium of hepatocarcinoma cells
在大腸桿菌blzi中實現了scfv融合蛋白的表達。經ni nta柱純化獲得純化產物，經間接elisa分析確定所得產物具有與hbsag結合的特異性。 -
The large - scale expression product is concentrated and desalted by ultra - filter, and the target protein is separated by affinity chromatography with chitin, and then cut by cnbr. the purified product appears evident antibacterial activity with method of agar diffusion
採用優化的表達條件進行大量發酵，對上清液超濾濃縮脫鹽后，採用幾丁質基質進行親和層析分離目的蛋白，溴化氰切割，瓊脂孔穴法測抗菌活性。
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