r-pcr 中文意思是什麼

r-pcr 解釋
普通聚合酶鏈反應,普通pcr
  • r : 1 〈拉丁語〉 recipe 【醫學】處方。2 radical 3 Reaumur 4 〈拉丁語〉 Rex Regina 5 radius 6 ratio 7 ...
  • pcr : PCR ＝polymerase chain reaction 【生物化學】聚合酶聯鎖反應〈此項技術可用以復制犯罪現場發現的DNA小樣，從而有助於破案〉。
  1. The divergent sequences of rdna from s. costatum are used to design primers meeting the requirements of the rfq - pcr. seven pairs of primers are designed and designated as primer 1 ( f / r ) ~ primer 7 ( f / r ), respectively, among which primer 1 ( f / r ), primer 2 ( f / r ), primer 5 ( f / r ), primer 6 ( f / r ) showed high level of specificities to s. costatum. then, the pcr products primed by primer6 ( f / r ) are sequenced

    首先以中肋骨條藻的rdna序列為設計種特異性引物的靶區域，共設計出7對適合rfq - pcr的引物（依次命名為primer1 （ f r ） primer7 （ f r ） ） ，並用常規pcr實驗初步證明其中有4對引物（ primer1 （ f r ） 、 primer2 （ f r ） 、 primer5 （ f r ） 、 primer6 （ f r ） ）可作為中肋骨條藻特異性引物的候選者；然後測定了以primer6 （ f r ）為引物的pcr產物的序列，序列分析表明，中肋骨條藻的pcr產物序列與其他藻的pcr產物序列差別較大，從中可設計出滿足rfq - pcr需要的taqman探針（命名為taqman6 ） ；進一步的核酸雜交實驗表明， taqman6隻與中肋骨條藻的pcr產物雜交，不與其他藻的pcr產物雜交。
  2. Here we managed to make cultured mice peritoneal macrophages be directly influenced by oligochitosan, and be stimulated by ifn - r before oligochitosan added, then measured the changes of gene transcription and translation level of both il - 1 and imf - a, respectively by methods of relatively quantitive rt - pcr and elisa. first, rt - pcr results showed that 18 hours was the most effective time and 40ug / ml was the most effective concentration of oligochitosan, then by the same method, confirm that 4hours is the most effective time and loou / ml is the most effective concentration of ifn - r stimulating. because ifn - r can enhance il - 1 and tnf - a gene expression of macrophages alone, so add ifn - r to microphages alone for 22 hours, then examined by rt - pcr, the results showed that il - 1 and tnf - a gene expression have no remarkable difference compared with the blank contrast group

    此外，由於ifn y單獨作用也可促進兩種細胞因子基因表達，故在巨噬細胞中加入ifn y單獨作用22h ，再經阿一pcr檢測，發現加ifn y的實驗組細胞的幾一lp和tnf a基因轉錄水平與空白對照組相比較無顯著性差異，可見，殼寡糖和ifn v對巨噬細胞il lp和tnf一口基因轉錄水平的影響在作用時間上無一致性，在殼寡糖作用最適時間時，僅受ifn y刺激的巨噬細胞il lp和tnf q基因轉錄己下降至刺激前水平，因此可以認為， ifn y的加入僅起到對巨噬細胞預刺激使之處于敏感狀態的作用，有利於增強殼寡糖對巨噬細胞的作用。
  3. They were capable of detecting 50 pg of purified total rna by a colorimetric assay and 5 pg by a chemiluminescent assay. the nonradioactive probe produced with pcr amplification were particularly suitable for practical diagnosis, as they are sensitive and can be rapidly prepared in large quantities. the two assay methods were 260 times and 26 times as sensitvity as r - page respectively

    利用含pstvd單體的pgem - 7z質粒，通過pcr擴增技術，用生物素標記制備cdna探針，進行雜交反應檢測pstvd ，其中通過化學顏色反應進行判讀靈敏度可達到50pg ，而用化學發光反應進行判讀靈敏度可達到5pg ，分別是r - page檢測靈敏度的260倍和26倍。
  4. Thereafter, the rfq - pcr method for the detection and enumeration of s. costatum cells is established with primer6 ( f / r ) and taqman6. the regression curve for enumeration is delineated according to the development of the fluorescent densities in the rfq - pcr with the increasing number of s. costatum cells. the regression equation is y = - 3. 3427x + 43. 443, in which x indicates the log10 of cell number, and y indicates the ct values, with r2 of 0. 9788

    以rfq一pcr中實際細胞數的常用對數值為橫座標，以測得的ct值為縱座標，繪制出了定量檢測的標準曲線，該曲線的回歸方程為: y =一3 . 3427x + 43 . 443 ，其相關系數是: rz二0 . 9788 ， ct值的標準估計誤差為sy 』 x二0 . 6741 。
  5. Conclusion we have constructed the expression plasmid pbv220 - hpf4 successfully. 3 ' - utr of h pf4 c dna was deleted and tag was mutated to taataa by pcr. after sds - page and densitometric scan analysis, the expression level of r hpf4 is 25 - 30 %

    結論本研究運用pcr定點突變技術，完全去除了hpf4cdna基因3 」端utrat富含區：改用大腸桿菌強串聯終止密碼子taaaataa ，成功構建高效表達克隆pbv220 rhpp4 。
  6. Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells, we design a pair of particular primers, and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic. the expression plasmid was identified with pcr and dna sequencing. pbv220 - r hpf4 was transformed into e. coli dh5a, bl21 ( de3 ) and induced by increasing the temperature to 42. we identified the expression protein by sds - page and western - blotting

    目的1人血小板因子4 （ hpf4 ）原核高效表達克隆的構建2重組hpf4的表達及分離、純化工藝研究3重組hpf4的特性研究方法根據原核細胞表達真核蛋白的基因表達調控特點，設計合成一對特異引物，在pt7 - 7 - rpf4表達質粒的基礎上，應用聚合酶鏈式反應（ pcr ）對其cdna進行改造，通過dna重組技術構建成重組hpf4原核表達質粒pbv220 - rhpf4 ，用快速pcr檢測法、 dna測序分析，鑒定重組hpf4表達質粒的正確性。
  7. Total rna was extracted from hepatic cells of mouse. a ecori and bamhi restriction sites were introduced into endostatin gene at specific primer f r, and endostatin was amplified by rt - pcr, this endostatin gene contained bamhi and ecori restriction sites at its 5 " and 3 " ends respectively

    從小鼠肝臟細胞中提取總rna 。設計合成一對特異引物，分別帶有ecori和bamhi的限制性內切酶的識別位點。用rt - pcr法擴增endostatin的基因片段，在endostatin基因的兩側引入ecori和bamhi酶切位點。
  8. All the samples with typical potato spindle tuber viroid ( pstvd ) symptoms collected from the fields in the northeast region of china were detected by the r - page method. the positive samples were verified. then, two - dimensional page and rt - pcr techniques were adapted in deleting pstvd

    對采自中國東北佳木斯、大慶等地區具有典型馬鈴薯紡錘塊莖類病毒（ pstvd ）癥狀的薯塊樣品進行往返電泳（ r - page ） ，確定含馬鈴薯紡錘塊莖類病毒的陽性樣品。
  9. Pcr fragments of 668bp, containing the quinolone resistance - determining region of the gyra gene got from the whole strains in the cherosome, and the one in the plasmid which were the first found in the salmonella typhimurium that reported in p. r. china

    結果表明， 16株致病性沙門氏菌對12種常用抗菌素的耐藥率在31 . 3 - 93 . 8之間，並已經出現了氟喹諾酮類耐藥菌株。
  10. Three binary expression vectors were constructed, which harbored the cl - i - 2kl, r - 2kl, and d - l - 2kl - r - 2kl respectively, driven by the camv 35s promoter. then, agrofeac / erium - mediated transformation of iobacco ( nicotiana tabacum, w38 ) was carried out. transformed tobacco lines were obtained through tissue culture and confirmed by pcr and southern blotting analysis

    通過分子克隆技術，將c1 - i - 2k1 、 r - 2k1和二者的連接（ c1 - i - 2k1 ? r - 2k1 ）分別構建到雙元表達載體上，並使它們分別置於35s啟動子的驅動之下，然後通過農桿菌介導的技術分別對煙草（ nicotianatabacum ， w38 ）進行了轉化，利用組織培養技術再生植株。
  11. Experiments of nucleic acid hybridization show that taqman 6 only hybridizes to the pcr products amplified from s. costatum, but not to products from other microalga. these results indicate that primer6 ( f / r ) and taqman6 can be used to establish the rfq - pcr method for the quantitative detection of s. costatum

    最後以primer6rdna序列在幾種浮游植物的分類及中肋骨條藻定量檢測中的應用（ f / r ）和taqman6探針建立了定量檢測中肋骨條藻的rfq一pcr 。
  12. Zeng y r, zhang m. improvement on ddrt - pcr and its application to studies of woody plants

    曾燕如張鳴差異顯示的改進及其在木本植物研究中的應用
  13. Zeng y r, cui y l. progress in ddrt - pcr. acta agriculturae universitatis jiangxiensis. 2002, 24

    曾燕如崔永蘭差異顯示研究進展江西農業大學學報2002 ， 24 6
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