r-plasmid 中文意思是什麼
r-plasmid
解釋
r質體,r質粒,耐藥質粒-
Conclusion we have constructed the expression plasmid pbv220 - hpf4 successfully. 3 ' - utr of h pf4 c dna was deleted and tag was mutated to taataa by pcr. after sds - page and densitometric scan analysis, the expression level of r hpf4 is 25 - 30 %
結論本研究運用pcr定點突變技術，完全去除了hpf4cdna基因3 」端utrat富含區：改用大腸桿菌強串聯終止密碼子taaaataa ，成功構建高效表達克隆pbv220 rhpp4 。 -
Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells, we design a pair of particular primers, and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic. the expression plasmid was identified with pcr and dna sequencing. pbv220 - r hpf4 was transformed into e. coli dh5a, bl21 ( de3 ) and induced by increasing the temperature to 42. we identified the expression protein by sds - page and western - blotting
目的1人血小板因子4 （ hpf4 ）原核高效表達克隆的構建2重組hpf4的表達及分離、純化工藝研究3重組hpf4的特性研究方法根據原核細胞表達真核蛋白的基因表達調控特點，設計合成一對特異引物，在pt7 - 7 - rpf4表達質粒的基礎上，應用聚合酶鏈式反應（ pcr ）對其cdna進行改造，通過dna重組技術構建成重組hpf4原核表達質粒pbv220 - rhpf4 ，用快速pcr檢測法、 dna測序分析，鑒定重組hpf4表達質粒的正確性。 -
Pcr fragments of 668bp, containing the quinolone resistance - determining region of the gyra gene got from the whole strains in the cherosome, and the one in the plasmid which were the first found in the salmonella typhimurium that reported in p. r. china
結果表明， 16株致病性沙門氏菌對12種常用抗菌素的耐藥率在31 . 3 - 93 . 8之間，並已經出現了氟喹諾酮類耐藥菌株。 -
Results 1 dna sequencing demonstrated that we have constructed the expression plasmid pbv220 - r hpf4 successfully
結果1dna測序證明pbv220 - rhpf4表達質粒構建成功。
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