random mutagenesis 中文意思是什麼
random mutagenesis
解釋
無規突變誘變- random : n 〈罕用語〉胡亂行為，偶然的[隨便的]行動[過程]。adj 1 任意的，胡亂的，隨便的；（話等）信口亂說的...
- mutagenesis : n. 突變。
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Dominant negative alleles of gpil7p were produced by random pcr mutagenesis of three conserved fragments of gpi17p : 1 ) having generated the plasmid library which contains 100000 plasmids harboring gpi17 mutants then screened from 8000 clones, we got 25 strong and 3 very strong dominant negative alleles
對gpi17p可能的三個保守區進行了pcr隨機誘變，並最終獲得顯性負性等位基因： 1 ）組建了gpi17p突變體質粒庫，其所含質粒數約為100000 。 -
A mutant library of xanthomonas campestris pv. campestris ( hereafter xcc ) strain 8004 with 17820 clones was constructed by random transposon tn5gwsa5 mutagenesis, which cover 1800 predicted orfs of xcc genome
用轉座子tn5gusa5誘變野油菜黃單胞菌（ xanthomonascampestrispv . campestris以下簡稱xcc ） ，構建了xcc8004tn5gusa5插入突變體庫。 -
The mutant pel - d92l was expressed in pichia pastoris gs115, sds - page detection showed that the expression product pel - d92l - gs is different from pel - gs, and its " yield decreased dramatically, the themostability of pel - d92l - gs is also different from the pel - gs, but their optimum temperatures are same. 3. directed evolution of pel through random mutagenesis mutagenesis pcr carried out in error - prone conditions was used on the vector psk - pel, using the oligos " beginning " and " end ", homologous to the 5 ' and to the 3 " ends of the gene of pel respectively
三、 pel基因的隨機誘變用易錯pcr方法對pel基因進行隨機誘變， pcr產物與ppic3 . 5k連接，轉化大腸桿菌，獲得的混合質粒電轉化畢赤酵母gs115 ， omm平板篩選適于低溫或對熱穩定的重組子，篩選獲得一株最活反應溫度、熱穩定性、發酵酶活均有提高的突變體pel - ep5 - gs ，其最活反應溫度為45 ，比野生型高出5 ； 40處理30min殘留活性為56 ，大大高於野生型的6 ；初始ph7 . 3528條件下培養72h ，發酵上清酶活為325u / ml 。
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