random primer 中文意思是什麼

random primer 解釋
隨機引物
  • random : n 〈罕用語〉胡亂行為,偶然的[隨便的]行動[過程]。adj 1 任意的,胡亂的,隨便的;(話等)信口亂說的...
  • primer : n 1 初級讀本,初學書;初階,入門(書);(尤指宗教改革前的)小禱告書。2 【印刷】10點或18點活字的...
  1. The main results are shown as below : 1. 80 individuals from 4 populations ( 20 from each population ) of argopecten irradians were analyzed by using 20 random rapd primers. 132 rapd bands ranging from 230 to 2800bp were recorded with an average of 6. 6 bands gained by per primer

    採用20條隨機引物對4個群體80個個體(每個群體20個個體)進行了rapd群體遺傳多樣性分析,共擴增出132個位點,片段大小在230 - 2800bp之間,平均每條引物的擴增帶數是6 . 6條。
  2. Lastly by using the technique of dot blot hybridization, the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer. the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe. by comparing the diagnostic ways of nucleotide probe and fc, the technique of nucleotide probe were proved to have high sensitivity and speci fi city

    最後,用地高辛隨機引物法標記成momp基因核酸探針,斑點雜交檢測衣原體基因組dna ,靈敏度可達10pg ,且不能檢出其它病原體的核酸。將核酸探針法與補體結合反應法對衣原體感染的診斷進行比較,初步證明該探針具有較高的敏感性與較強的特異性。
  3. Four out of 150 random primers could detect dna polymorphism between the two mutants and the wild type arabidopsis thaliana, which provided a strong evidence for the truth of the mutants. only one primer gave a specific dna fragment about 1200bp which two mutants own but the parent do n ' t. we drew a preliminary conclusion that the fragment might be related to the salt - tolerance gene

    擬南芥抗鹽突變體的rapd分析結果表明, 150條引物中有四條引物在突變體和野生型擬南芥之間擴增出多態性,證明了dna水平突變的發生,其中1條引物在突變體的擴增產物比在野生型的擴增產物多出一個大小約為1200bp的片段,初步認為該片段可能與植物的抗鹽性有關。
  4. Rapd ( random amplified polymorphic dna ), which bases on the polymerase chain reaction ( pcr ), is by far one of the most commonly molecular techniques to uncover dna sequence polymorphisms. the basic priciple of this technique is that an arbitrary primer ( usually lobp oligonudetide ) is used to amplify random segments of dna, and a small number of fragments will be amplified when the primer anneals on each strand over a length range. if sequence variation is present at the priming site, then a fragment may not be amplied, so the dna polymorphic can be detected

    Rapd (隨機擴增多態性dna )技術是二十世紀90年代發展起來的一項dna分子多態性檢測技術,它建立於聚合酶鏈式反應( pcr )技術基礎之上,利用隨機合成的寡聚核苷酸序列為引物(一般為10個bp ) ,分別與dna的兩條單鏈結合,在dna聚合酶的作用下,對基因組的特定區域進行pcr擴增,其電泳結果為不同大小和數目的dna譜帶即rapd圖譜,可反映基因組相應區域的dna多態性。
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