reading strain 中文意思是什麼
reading strain
解釋
讀數電橋-
Marek " s disease virus ( mdv ) phosphoprotein 24 ( pp24 ) gene was amplified from md11 strain by polymerase chain reaction ( pcr ). then we cloned it into the downstream of gst gene according to the right open reading frame ( orf ) in pgex - 6p - l vector
本研究將型mdvmd11株的pp24基因的完整orf克隆入原核表達載體pgex - 6p - 1中，重組質粒pgex - pp24轉化bl21宿主菌后，經iptg誘導表達。 -
The principal advantage of the photoelastic strain gauge is that it is directed reading self-contained.
光彈性應變計的主要優點在於它是直讀而整裝的。 -
The sequence 56 analysis shows that there is a long open reading frame in it which encode a protein of 364 animo acid residues. the m protein include 47 basic amino acid, 30 acidic amino acid, which cause a basic estimated pi of 9. 7. phylogenetic tree based on ndv matrix protein gene shows that the b95 and v4 has the most close relationship than other ndv strain
關于ndvm蛋白國內未見有報道，國外對此蛋白的研究也十分有限，本文克隆出了ndv株b95m蛋白基因的全序列，為m蛋白的表達及進一步研究其在病毒復制與致病中的作用機理打下了基礎。 -
Sequence analysis indicated that the opening reading frame of ns1 gene is 1056nt in length, encoding 352 amino acids, identical to the sequence of strain sa 14 - 14 - 2 deposited in genbank. ns1 gene in the pmd18 - t - ns1 was cut by bamhi and hindiii, was cloned into the expression plasmid pet - 30b ( + ) treated with the same enzymes, resulting in a recombinant plasmid pet - 30b - ns1. the pet - 30b - ns1 was identified by pcr, restriction digestion, and sequencing
通過序列分析結果表明， jev - ns1基因編碼區核苷酸序列長度為1056bp ，編碼352個氨基酸，序列分析和比較表明本實驗克隆到的ns1蛋白基因與genbank中收錄的疫苗株sa14 - 14 - 2的核苷酸序列一致，表明盡管該疫苗株在我國應用多年，但ns1基因並未發生變異，提示該疫苗株遺傳性狀比較穩定，是一株優良的疫苗株。 -
A bt - e. coli shuttle vector pht315 was deleted its replication region of bt, then constructed a novel vector named pht315 - 1 which composed a multiple cloning site, erythromycin and ampicillin - resistance marker and could only replicated in e. coli. used pht315 - 1, a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324. sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501, 333, 183aas. orfl had 98 % identities with replicating related protein ori43 of bt strain hd263. the others were no homology to any published bt replicating related protein. after continuous cultured for 70h at 30 c without antibiotic selecting press. the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 %. and growth curve also showed that the novel replicon was stable and could replicate normally
進一步序列分析表明該復制區至少有3個較大的orf ，分別編碼501 ， 333 ， 183個氨基酸。其中orf1蛋白序列與hd263復制蛋白ori43的同源性為98 ，而另外兩個orf和genbank己公布的bt復制相關蛋白無同源性。 30連續培養72h ，復制區質粒在bt無晶體突變株hd73cry ~ -中穩定性達98以上， 30h生長曲線也表明該復制區能夠在bt中穩定復制和遺傳，對受體菌株無明顯不良影響。 -
The comparison of meq gene sequences of different pathotypes of marek ' s disease virus meq genes of different pathotypes of marek ' s disease virus ( mdv ) were amplified, in the whole opening reading frame ( orf ) by polymerase chain reaction ( pcr ) technique, sequenced and compared with the published sequence of ga strain ( representing vmdv ). cvi988 / rispens and 814, commercial vaccine strains popularly used worldwide and in china respectively ; 648a, representing very virulent plus ( vv + mdv ) and 6 field isolates, originated from guangxi commercial chickens with visceral lymphomas and vaccine breaks, representing high pathogenic ( hpmdv ) and two of them ( g2 and n ) were proved to be vvmdv, were used
本研究通過三個方面進行了研究，取得了如下主要實驗結果： 1mdv不同致病型（ pathotypes ）毒株meq基因序列的比較研究應用pcr技術擴增了不同致病型mdvs ，即國際通用型疫苗毒cv1988 rispens株和中國特有的疫苗毒814株、特超強毒648a株（ vv + mdv ）以及6個廣西分離到的野毒株共9個毒株的meq基因，進行核苷酸序列的測定並與標準強毒ga株（ vmdv ）進行核苷酸和氨基酸序列的比較。 -
You will strain your eyes by reading in such poor light
你在這樣弱的光線下看書會損傷視力的。 -
There were six major open reading frames in genome of f48e9 strain which encode the viral np, p, m, f, hn and l protein, respectively
為了驗證該6個堿基是否只存在於強毒株及其與毒力的關系，我們又設計一對引物x1和x2 。 -
The results showed that the two isolates have more than 95 % homology with the snv strain. the env gp90 was amplified from the snv strain by pcr and was cloned into the downstream of gst gene in pgex - 6p - l vector according to the correct open reading frame, and this recombinant was transformed into bl21 for expression
結果發現，在核苷酸水平上，參考株snvenv基因與兩株中國分離株的同源性分別為97 . 7和95 ，而在氨基酸水平上，其同源性為96 . 9和92 . 7 。
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