recognized target 中文意思是什麼

recognized target 解釋
識別目標
  • recognized : 辨認，公認；招呼
  • target : n 靶子，標的；目標；（嘲笑等的）對象；笑柄 （for）; （儲蓄，貿易等的）定額，指標；小羊的頸胸肉；...
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. The standard system of the university performance audit must be established on economy, efficiency and effectiveness and constituted by the standard of economy and efficiency, the standard of effectiveness and the good system standard, in which the standard of economy and efficiency is reflected through funding structure, fund balance ratio, the resource use efficiency indicator and the fund use structure indicator ; the standard of effectiveness is reflected through educational standard, research capabilities, student training quality and social well - known and recognized degree ; the good system standard is reflected through school running thoughts, school target positioning, school regulation building, school

    高校績效審計標準體系必須圍繞經濟性、效率性和效果性來建立，由經濟性效率性標準、效果性標準、良好制度標準三部分組成。其中經濟性、效率性標準通過經費來源結構、經費結余比率、資源利用效率指標、經費使用結構指標四方面來反映;效果性標準通過辦學水平、科研能力、學生培養質量、社會知名和認可程度等指標來反映;良好制度標準通過學校辦學思路、學校目標定位、學校規章制度建設、學校教學質量監控及測評系統等方面來體現。
  3. 1 the forecast model of the load of the heating system was put forward, parmeter of the model was recognized in genetic algorithm. on the basis of the result, the temperature of water supply was calculated and become target aim of burning system

    針對集中供熱系統的特點，設計了整個系統的運行控制方案。 1建立了供熱負荷預測模型，採用遺傳演算法對模型的參數辨識，並以此來計算當天的供熱溫度，指導鍋爐的自動控制。
  4. Under the guidance of new principles and methods of modern sedimentology, detrital petrography, reservoir geology, diagenesis, this thesis has carried out detailed study on paleogene formation correlation, sedimentary environment, characteristics of sedimentary facies and diagenesis of the eastern kuche depression, tarim basin, dina area being taken as the main target of the study. based on the core, geology logging, log and seismic data from 9 typical wells, combined with the data of the adjoining area, the dominant sedimentary types of the study area have been recognized and illustrated, such as fan - delta, lacus and normal delta. in terms of the analysis on the sedimentary facies of typical and connecting wells in different areas and facies belt, together with the paleogene tectonic evolution characteristics of foreland basin, the distribution and evolution of the sedimentary facies of the study area have been clearly discovered both vertically and laterally

    通過對該區9口井的巖芯、錄井、測井、地震資料及鄰區資料的分析，識別並闡述了庫車坳陷東部下第三系的幾種主要的沉積相類型，如扇三角洲相、湖泊相和正常三角洲相；通過對不同地區、不同相帶典型井及連井的沉積相分析，結合前陸盆地在下第三系的構造演化特點，弄清了迪那地區下第三系沉積相的縱橫相發育展布和演化規律，並建立了沉積相模式；同時，初步開展了成巖作用、儲層孔隙演化和儲層發育模式的研究工作，科學合理地解釋了現今迪那地區下第三系儲產層主要發育在粉砂巖中的特殊現象，首次建立了下第三系儲層的發育演化模式，為庫車坳陷東部地區進一步尋找下第三系大儲量、高產油氣藏和有利的勘探區塊提供科學的、可靠的和最直接的地質依據。
  5. The homologious comparison proved the cloned gene had 96 % homology to the sequence of the omp gene, and the alignment of the amino acid sequence was 98 %. the recombinant plasmid was constructed with the target gene and the expressing vector pgex - 4t - l and then was transformed into e. coli bl21 ( de3 ) the fusion protein was expressed under the iptg inducing condition, and exhibited about 62kda in size, very close to the predicted molecular weight of gst - momp. furthermore, the fusion protein was specifically recognized by anti - serum which raised against the major outer membrane protein of ahl316

    Sds - page電泳分析顯示誘導表達的基因產物分子量約為62kda ，與預測的gst -外膜蛋白重組融合蛋白的分子量極為相似， western - blot進一步證實，表達產物能被嗜水氣單胞菌l316主要外膜蛋白特異性抗血清所識別，產生明顯的染色條帶，說明所表達的基因產物與天然的外膜蛋白抗原性一致。
  6. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  7. Corporate culture, with their own specified value orientation, behavior style, management concept, company spirit, moral regulation, development target, and idea through, which is gradually formed in the course of a long term manufacture and management, should be recognized by all the staffs

    企業文化是企業在長期的生產經營活動中逐步形成的，並為全體員工所認同的，具有本企業特色的價值取向、行為方式、經營理念、企業精神、道德規范、發展目標和思想意識等因素的總和。
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