recombinant chromosome 中文意思是什麼
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The positive transformants with the integrates mn - sod gene was identified by zeocin - resistance, pcr screening and expression in p. pastoris. the recombinant mn - sod protein was successfully expressed in pichia pastoris based on the evidences that a relative molecular weight about 23kd appeard in sds - page, the obvious activity of sod existed in native - page and enzymatic activity test, and mn - sod activity was specific base on the inhibition with the mixture of chloroform - enthanol ( 3 : 5 / v : v ) and potassium cyanide. two secreted plasmids ppiczaa - sodm18 and ppiczaa - sodc were constructed and after there linearization were transferred into chromosome of pichia pastoris gs115 by electroporation
Pcr鑒定及mut表型分析進一步說明，目標基因已經重組到宿主菌基因組染色體上； 0 . 5甲醇誘導表達后， sds - page結果顯示，表達的蛋白相對分子量約為23kd ，活性電泳出現明顯活性條帶；酶活性測定顯示，重組菌株sod活性比對照提高5倍左右；氯仿-乙醇（ 3 : 5 v : v ）和kcn （ 5mmol l ）抑制反應進一步證明，所表達的sod為錳超氧化物歧化酶。
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