recombinant genome 中文意思是什麼
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Methods : an artificial gene for fl extracellular domain cdna was synthesized by using favored genetic codons of pichia pastroris. by inserting human fl extracellular domain cdna coding 156 amino acid resi dues into pichia pastoris expression vector ppic9k containing aox1 promoter and the sequences of alpha secreting signal peptides, a recombinant expression plasmid ppic9k - fl was constructed, and integrated into the alcohol oxidase region of the host genome
為了提高外源基因的表達量，我們根據畢赤氏酵母偏愛密碼子人工合成了編碼fl胞外區156個氨基酸的cdna序列，目的序列被定向克隆到酵母分泌型表達載體ppic9k質粒上，構建ppic9k - fl表達質粒。 -
Using enterobacter cloacae b8, the mutated strains b8b and b8f, and the recombinant clones pb and pf, we try to sequence the antagonistic - related genes of enterobacter cloacae b8 by subcloning and genome primering system. the acquired sequences were analyzed with blast program to find any homology to sequences deposited in genebank
以廣譜拮抗菌陰溝腸桿菌b8菌株和拮抗活性缺失菌株b8b 、 b8f及從b8b和b8f二菌株克隆獲得的重組質粒pb 、 pf為基礎，對陰溝腸桿菌b8菌株拮抗相關的b和f基因片段進行序列分析。 -
The result demonstrated that the ctbvpl fusion gene was inserted into the c. reinhardtii chloroplast genome and after three rounds of spc selection the recombinant chloroplast dnas were the majority, and the wild - type chlorop last dnas
W七sternblot和elisa免疫雜交分析表明ctbvpi融合蛋白在衣藻葉綠體中得到表達，表達量占可溶性總蛋白量的3一4 % （ lmg可溶性總蛋白含有30一40pg的重組蛋白） 。 -
Using the monoclonal antibody to rev and the anti - sera against the rev env gp90 - gst fusion protein. the molecular cloned virus was detected by ifa. we also amplified the gp90 from the cells infected with the molecular cloned virus by polymerase chain reaction. all these results indicated the recombinant plasmid containing the total rev genome cdna is infectious
對snv株前病毒全基因組cdna克隆進行酶切，將酶切產物分別克隆進puc18中，分別將各個亞克隆進行測序，按照酶切位點和已知的部分序列以及rev物理圖譜將測得的序列進行拼接，完成了rev全基因組序列。 -
A dna fragment was amplified from lactococcus lactis nizo r5 genome by means of pcr and cloned into pmg36e plasmid. the recombinant plasmid was transformd into e. colijm 109 and identified by restriction endonucleases analyzing. the recombinant plasmid was introduced into lactococcus lactis nz9800
本實驗提取乳酸乳球菌nizor5的基因組dna為模板，用pcr方法得到乳鏈菌肽前體基因（ nisa ） ，克隆到乳酸乳球菌表達載體pmg36e ，構建了表達載體pmg36e nisa 。 -
This study we acquired the coding region of hcv ns5b gene by pcr of hcv full length genome and construct the recombinant plasmid pegep n3 - ns5b ; with the different concentration of g418 in the culture medium, we think the selection concentration of g418 for hepg2 cell is 800 g / ml ; the recombinant plasmid was transfected into hepg2 cell by lipofectamine2000 cells containing stable transformants were selected by the ability of resistance to g418 and isolated with the limited dilution. the stably transfected cell line expressing ns5b - egfp fusion protein was obtained by the detection under fluorescence microscope and rt - pcr
本研究首先從hcv基因組中擴增出nssb基因，構建了nssb基因與報告分子egfp （增強型綠色熒光蛋白）基因的融合基因真核表達質粒pegfpn30 ；通過含有不同g418濃度梯度的培養液培養hepgz細胞，確定了篩選用g4181作濃度為800pg ml ；利用脂質體法將該重組質粒轉染hepgz細胞，經過有限稀釋法和g4壓力選擇，應用熒光顯微鏡和rtpcr檢測，獲得可穩定表達nssbegfp融合蛋白的hepgz細胞克隆。
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