recombinant strain 中文意思是什麼

recombinant strain 解釋
重組株
  • recombinant : n. 【遺】重組器官，復合器官。
  • strain : vt 1 用力拉，拉緊，抽緊，扯緊。2 使緊張；盡量使用（肌肉等）。3 強迫，強制；濫用，盡量利用。4 拉傷...
  1. Since the important roles of eo protein in the viral infection. immunity and virus - host interaction. the homology of 21 csfv strains was investigated by sequence analysis of eo genes in this study, which will provide some evidence for epidemiological study. in addition, the eo gene of hog cholera lapinized vaccine ( hclv ) strain was expressed in the prokaryotic and eucaryotic systems, and the recombinant proteins were preliminarily analyzed by immunological method

    鑒于eo蛋白在病毒感染，誘導機體免疫及與宿主細胞相互關系中的作用，本研究克隆了2株豬瘟病毒eo基因並將其與其它毒株eo基因進行了序列分析，揭示了我國豬瘟病毒流行株之間的遺傳演化關系，為豬瘟病毒的流行病學研究提供依據。
  2. 5. exploring fermentation synthesis of quinic acid ( qa ) using bioengineering strain under shake flask conditions synthesis of qa using recombinant e. coli constructs was examined in m9 accumulation medium. the result indicated that qa was produced from d - glucose in the culture supernatant, but it is necessary to explore further the consequences of fermentation in next work

    工程菌搖瓶發酵產酸初步觀察初步探索了奎尼酸工程菌在m9積累培養基中的發酵產酸條件，結果表明工程菌經直接發酵由葡萄糖產生了奎尼酸，但尚須進一步優化發酵條件提高產酸率。
  3. The recombinant b. thuringiensis subsp. israelensis b - pmpx2 can produce a rhombus crystalline inclusion body during its sporulation sds - page analysis proved that cytlaa had overexpressed during the sporulation of the recombinant. it was found that b - pmpx2 strain remained stable toxicity, whatever during vegetative phase or sporulation phase, which was similar to the expression of mtxl gene in the strain of protease ( s ) - deficient b. sphaericus

    同時，將來源於蘇雲金芽孢桿菌以色列亞種（ b . thuringiensissubsp . israelensis ， b . t . i ）的cytlaa基因和p20伴侶蛋白基因克隆連接到質粒pmt9中，並轉化到蘇雲金芽孢桿菌無晶體型中得到重組轉化子b - pmpx2 ，電鏡觀察發現重組轉化子b - pmpx2形成一菱形晶體。
  4. 97 % identities in amino acids respectively. the e. coli strain dh5 transformed recombinant plasmid phn was induced with 0. 6 mmol / m iptg for n gene expression. the expressed product was identified by sds - page and westem - blot test, a fusion protein about 47ku as we expected was found

    將含有重組質粒phn的菌株dh5在37條件下培養，以濃度為0 . 6mmol / liptg誘導，重組質粒n基因phn融合蛋白獲得了表達：經sds - page ， western - blot試驗，確定其表達的融合蛋白產物大小為預期的47ku 。
  5. Construction of recombinant fowlpox virus coexpressing aiv ha and ndv f. for the construction of transfer vector pfgs11haf, aiv ha gene of f strain in puc18ha and ndv f gene of f48e8 strain in puc19f were removed and inserted into pfgs11. recombinant fowlpox viruses ( rfpv ) coexpressing aiv ha gene and ndv f gene were constructed by using different promoters of ps and pe / l. recombinant rfpvs were derived by dosper liposome - mediated transfection with the two transfer vectors on chicken embryo fibroblast ( cef ) monolayer cultures which were infected by wild type fpv chinese vaccine strain 282e4 3 - 4 hours earlier

    Puc18ha和sk質粒同時經hind 、 kpn酶切后連接得中間質粒skha ；將質粒skha用bamhi酶切回收ha基因插入到插入載體pfgs11中的bamhi位點，通過酶切鑒定獲得了pfgs11ha ；將含ndvf基因的質粒puc19f用hind 、 sal酶切經klenow酶補平插入到經sma酶切后的skifn中pe / l啟動子下獲得中間質粒skf ，再將質粒skf和puc18質粒先分別用ecor 、 xho酶切klenow補平，后再共同用sac酶切連接得puc18pelf ， sal酶切回收pe / l - f基因盒插入到pfgs11ha的sal位點，通過酶切鑒定獲得了pe / l - f與ps - ha同向的表達載體pfgs11haf 。
  6. In this study, the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome, which have the lacz reporter gene, earlier / latter promoters lp2ep2 of fpv, promoters p7. 5 and p7. 1 of vaccinia virus, replication unnecessary region of fpv - 017. following 6 cycles screenings, clonings, purification of blue plaques, detection of pcr and dot - elisa, which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully. this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine, as well as provided substance preparatory for prevention the high mortality gpv

    本研究採用脂質體轉染方法，將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的轉移載體質粒psy681vp3lacz與fpv - 017共轉染雞胚成纖維細胞，經6輪蝕斑克隆、篩選、表達， pcr鑒定和dot - elisa檢測，證明該重組病毒已構建成功，並獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。
  7. The result shows that a vvibdv strain was obtained, the above work lay a important role for further studying on the molecular biological mechanism of antigenic drift and virulence variation of ibdv, molecular epedimiology, it also provided the basis for recombinant and gene deleted vaccine of ibdv

    本實驗可以幫助我們進一步探討ibdv抗原性漂移和毒力變化的分子生物學機制，追溯ibdv的起源，理解病毒的傳播方式。同時也為研製開發基因重組疫苗和缺失疫苗打下一定的基礎。
  8. By recombinant dna techniques, the vp2 gene of gpv hi strain was fused in frame with 6his gene of prokaryotic expression vector pproex - htb. the recombinant expression plasmid of goose parvovirus vp2 gene pproex - htb - vp2 was transformed into e. coli dh5a and induced with iptg. sds - page analysis showed an induced expression product band about 72ku, which correspond to the sizes of vp2, reported in the literature

    利用dna重組技術，將其結構蛋白vp2基因亞克隆至原核表達載體pproex - htb ， iptg誘導后成功表達出與預期大小相符的約72ku的融合蛋白，光密度掃描對表達產物進行初步定量，表明表達產物約占菌體總蛋白的14 。
  9. The recombination vectors were transformed into host strain of bl21 and induced with iptg. all the recombinant protein was expressed into inclusion body. the recombinant protein was identified with sds - page and westen - blot and purified through elution method. the muticlone antibody was got by immuning the rabbit with the purified protein

    把重組質粒轉化入表達受體菌bl21 ，經iptg誘導后都獲得了表達，且都以包涵體的表達形式存在，用sds - page和western - blot的方法對重組蛋白進行了鑒定。
  10. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  11. Both the mature genes of gloshedobin and gussurobin were cloned into the vector pet - 32a ( + ), strain bl21 ( de3 ), to study their expression in prokaryotic cell. the gene was expressed under t7 promoter with a fusion protein partner of thx. tag and a 6x his. tag at its n - terminal. having been induced by iptg for 4 hours, the recombinant enzyme was examined in the cytoplasm by sds - page analysis

    將大連蛇島蝮蛇和長白山白眉蝮蛇毒類凝血酶基因分別克隆到大腸桿菌表達載體pet - 32 （ a ） +中，在t7啟動子下表達出融合蛋白，融合部分為硫氧還蛋白，位於類凝血酶基因上游，並在其n端帶6xhistag標簽以利於表達產物的分離純化，經熱激轉化至宿主菌bl21 （ de3 ）中， iptg誘導斗小時后收獲菌體。
  12. Construction and identification of recombinant attenuated salmonella typhimurium vaccine strain expressing helicobacter pylori urease subunit b

    重組減毒鼠傷寒沙門氏菌的構建和鑒定
  13. Construction and identification of a recombinant attenuated salmonella typhimurium vaccine strain expressing helicobacter pylori hpaa gene

    亞單位的重組減毒鼠傷寒沙門菌核酸疫苗的構建
  14. Expression of cyt1aa from bacillus thuringiensis in bacillus thuringiensis var. tenebrionis strain and the insecticidal activity of the recombinant strain

    基因在擬步甲亞種菌株中的表達及重組菌株殺蟲活性研究
  15. It was confirmed that the key enzyme of coq10 synthesis is p - hydroxybenzoate polyprenyltransferase, encoded by gene ubia in e. coli and lacks specificity to the aggregation length of its substrate ( polyisoprenyl pyrophosphate ). if gene ubia can be introduced into psb and is high expression in psb, a recombinant strain producing coq10 could be obtained. consequently, the productive cost will decrease sharply

    業已證實coq _ （ 10 ）生物合成的關鍵酶為對羥基苯甲酸聚異戊二烯焦磷酸轉移酶（在大腸桿菌中該酶由ubia基因編碼） ，該類酶對底物聚異戊二烯焦磷酸（ ppp ）的聚合長度並無特殊要求。
  16. The gene recombinant strain no. 42 ca n ' t generate ampramycin, which indicated that the cloned gene is involved in apramycin biosynthesis in s. tenebrarius

    通過接合轉移的方法將質粒pzxb014導入黑暗鏈黴菌h6中，篩選基因發生重組的菌株。
  17. However, the content of coq10 in wild strains of psb is limited after all. through construction of recombinant strain producing coq10 and optimization of the culture medium and fermentation conditions of a selected strain, the content of coq10 in psb will be increased markedly. it can not only remarkably strengthen the effect of psb feed additive, but also lay foundation for the exploitation of coq10

    但野生型psb菌株coq _ （ 10 ）含量終究有限，如能選擇合適菌株進行培養優化，並通過構建基因工程菌，使菌體中coq _ （ 10 ）的含量成倍提高，則不僅可顯著增強psb飼料添加劑的應用效果，更重要的是，為coq _ （ 10 ）相關產品的研發奠定良好的基礎。
  18. One highly productive and genetically stable recombinant strain named e - 22, which produced phytase with 143958. 3u / ml under the condition of flask cultivation, was selected through further screening. the phytase activity of e - 22 was 341. 13 times as high as that of the original strain ( 422u / ml )

    經搖瓶復篩后得到l株產酶活性為143958 . 3uzml發酵液，並且具有良好遺傳穩定性的高產工程菌株（ e22 ） ，其產酶活性是出發菌株酶活性（ 422u / ml ）的341
  19. The pichia pastoris recombinant strain was induced by methanol addition to the culture medium

    Sds - page分析表明，在63kd處有明顯的蛋白質特徵帶。
  20. Object : the culture medium and culture conditions of psb will be optimized and gene ubia will be cloned for the construction of recombinant strain producing and foundation of the fermentative technology in large scale

    目的：優化psb產coq _ （ 10 ）培養基及培養條件和克隆ubia基因，為獲取高產coq _ （ 10 ）基因工程菌及確定規模化發酵工藝奠定良好的基礎。
分享友人
分享到 Line

分享到臉書