recombination gene 中文意思是什麼
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Recombination gene was cut - down and introduced into the nuclei of oocytes or the cytoplasm of goldfish at one - cell stage via microinjection. the results as follows : ( 1 ) fluorescence was observed from embryo under suitable uv light after microinjection 36 hours. the fluorescent ratio of gastrula embryo period was up to 25 %
採用顯微注射法將這種重組基因轉化1細胞期的金魚受精卵，實驗結果如下： （ 1 ）顯微注射后，根據胚胎發育分期，胚胎在顯微注射后36小時開始能在紫外燈下觀察到熒光，原腸期發熒光的胚胎比例為25 ，後期發育熒光率逐漸下降，肌肉效應期后又相對穩定。 -
4. engineering dhqase ( arod ) - deficient e. coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e. coli chromosome. the mutant 31bk was engineered, in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e. coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system. the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene, so it improved carbon flow into the quinic acid biosynthesis direction
構建宿主菌基因精確定位突變株31bk （ arod : : arobkan ~ r ）為了改變代謝途徑脫氫奎尼酸（ dhq ）分支點上的代謝流量，使之充分流向目的產物奎尼酸合成方向，利用基因打靶技術構建了31884宿主菌arod基因精確定位插入突變體，使dhq脫水酶（ dhqase ）失活，阻斷了碳代謝流流向芳香氨基酸生成的方向，同時用同源重組的方法將arob基因定位整合入染色體上，解除了限速酶對碳代謝流通過共同途徑到達dhq的阻遏影響，並減輕代謝負擔。 -
Recent studies show that dna transfer and recombination technology provides great potential for genetic improvement of zoysiagrasses ; molecular linkage maps were constructed from zoysia japonica and its hybrids with zoysia matrella ; the researches on gene cloning and gene resource are in progress
最近的研究表明，基因轉移與重組技術在結縷草遺傳改良中具有巨大應用潛力；結縷草遺傳連鎖圖譜構建取得重要進展；基因克隆和基因資源研究正在展開。 -
The topics include : structure and function of genes, chromosomes and genomes, biological variation resulting from recombination, mutation, and selection, population genetics, use of genetic methods to analyze protein function, gene regulation and inherited disease
主題包括：基因、染色體與基因組的結構和功能；來自於基因重組、突變和篩選的生物變異；族群遺傳學；運用遺傳學的方法分析蛋白質的功能，基因的調控和遺傳性疾病。 -
The research adopts that hu - - ifn gene were introduced into the nuclei of oocytes or cytoplasm of grass carp to develop anti - disease transgenic grass carp breeding researches, combing the adva ntag e of hu - - ifn gene and breeding by genetic engineering, with an aim of finding out an effective way of solving antivirus of hemorrhagic virus of carp completely. in research of transgenic fish, hu - - ifn gene ( recombination gene ) is cutdown and introduced into the nuclei of oocytes or cytoplasm of grass carp at one - cell or two - cell stage via micyoinjection by narashige micyoinjection apparatus
本研究的目的在於以人的-干擾素基因（ ifn - ）作為目的基因，與鯉魚-肌動蛋白基因啟動子在體外重組，利用原核顯微注射轉基因技術將人-干擾素基因導入草魚基因組而開展的抗病轉基因草魚育種研究，其結合了干擾素和基因工程育種抗草魚出血病病毒的優點，以期獲得對草魚出血病具有天然抗性的轉基因魚，並在此基礎上培育出草魚抗病新品系。 -
Gene cassette could be integrated into an integron with recombination between atti and attc sites by integrase ( inti ), or excised from an integron and become a free circle molecule
基因盒可從整合子中切除成為游離的環狀分子，或者將外源基因盒捕獲並整合入整合子。 -
Thirdly, the hyl gene was cloned to puc19 and pbr322 respectively. then the fragment containing ampr derived from puc19 and hasa came from pse380 - has were inserted into hyl either or both in vitro. transformed the recombinated vectors into s. equi by electro - operation, then plating on hag solid medium containing ampicillin, and selected clones hi which the hyl gene was inactivated by gene replacement through homologous recombination
（ 1 ）直接插入外源基因滅活hyl方法：將hyl片段與載體puc19 pbr322連接后，用來自puc19的amp ~ r抗性基因片段從hyl基因的中部插入將載體上的hyl片段分為兩部分； （ 2 ）用hasa替換hyl部分片段的方法：將hyl片段與載體pucl9 pbr322連接后，用hasa片段替換部分hyl ，再將amp ~ r片段接入到hasa上游。 -
Integron consists of an integrase gene ( inti ), a integrase specific recombination site ( atti ), and gene cassettes with varied number
整合子由整合酶（ integrase ）基因（ intl ） 、整合酶特異性重組點atti和數目不定的基因盒（ genecassette ）組成。 -
A new resolution vector based on tnpi - mediated site - specific recombination system of b. thuringiensis transposon tn4430 was developed. the crylac10 or other target dna, and the gene ori1030, from a plasmid of wide type b. thitringiensis subsp
利用轉座因子構建解離載體的可行性利用蘇雲金芽胞桿菌轉座子tn4430的解離區構建了解離載體。 -
In the research of transgenic fish, green fluorescent protein gene was sub - cloned to downstream of carp p - actin gene promoter that was cloned in pucusa by molecular recombination technology. thus pagfp plasmid was constructed successfully. the recombination was determined by digestion of restriction enzyme and sequencing
實驗通過分子重組技術，採用定向克隆法將綠色熒光蛋白基因亞克隆到puc118a上鯉魚-肌動蛋白基因啟動子下游，構建成能在真核生物體內表達的表達載體pagfp ，經雙酶酶切法序列鑒定后，回收帶啟動子和目的基因片段。 -
To pick up the convergence speed of traditional genetic algorithm, a modified genetic algorithm is presented, which is based on subsection integer coding, combining stable - state selection strategy with inequality individual and scaling, adaptive recombination according to gene sufficiency, self - adaptive variable step and multi - gene mutation
將兩幅圖象重疊區域的歸一化差圖象作為搜索空間，定義一個與圖象高相等維數的向量作為染色體，染色體的基因表示每一行圖象中的最優拼接點，採用常用的最小值搜索適應度函數作為視差圖像拼縫搜索的適應度函數。 -
Present research on the sub - cell localization of expression product of exogenous recombination gene
外源重組基因表達產物亞細胞定位的研究現狀 -
The recombination gene micyoinjected is 5nl dna ( about 106 - 107 ) into everyone oocytes or cytoplasm of grass carp. there were 32197 oocytes of 12 series via micyoinjection and achived 12945 fries in 2000 year, by hatching and raising. genomic dna was extracted from grass carp who were transformated in 1120 grass carp presented postive reaction by blood
實驗通過將hu - - ifn基因重組分子採用顯微注射法將這種重組基因轉化草魚1 - 2細胞期的受精卵，即以narashige顯微注射儀，將5nldna （約10 ~ 6 10 ~ 7分子）直接注射到選擇好的受精卵核的動物極上。 -
Linkage analysis plays an important role in gene mapping. the foundation : the two gene locuses which locate on the same chromosomal ( eg. disease gene and marker gene ) happen to cross over and recombine. the farther the distance between two locuses is, the higher the probability happening to cross over is, the lower the probability that the two locuses are inherited to offspring together is, that is, the degree of linkage is not strong. so we can estimate the distance and the degree of linkage by the recombination fraction between the two locuses to locate gene
連鎖分析是基因定位主要策略之一，其基本原理是位於同一染色體上兩個基因位點（例致病基因與標記基因）在減數分裂的過程中會發生交換與重組，染色體上的兩個位點間距離越遠，發生重組的概率就越大，兩個位點在一起傳給後代的機會就越少，即連鎖程度弱，這樣由標記位點與疾病位點間的重組率可估算出兩者間的距離以及連鎖程度，達到基因定位的目的。 -
The research showed that their interactions in yeast two - hybrid system are still steady when the vector was exchanged, in contrast, the interaction was disappear when the reading frame of each positive had been changed. the four positives were subcloned into suicide plasmid so that gene mutant strains could be constructed via conjugation and homologous recombination
為了進一步驗證這些克隆的編碼產物與nifa蛋白的相互作用，將它們與nifa基因互換載體后共轉化酵母，仍然可以激活三個報告基因的表達，而陽性克隆移碼表達的重組質粒和pgbd - nifa的共轉化物則不能在選擇性平板上生長。 -
This expression vector plbcas - hsa - lgl has the following advantages : i ) the 1. 7kb promoter is able to drive cell - specific and hormone - dependent expression ; ii ) the inclusion of intron - 1 can increase expression level of fusion genes ; iii ) the 5 ' utr of bovine p - casein mrna may have a positive role in both transcriptional and post - transcriptional regulation ; iv ) the gfp gene make the selection of positive clone among embryos possible ; v ) the gfp gene can be easily excised via cre - mediated recombination between the two loxp sites after the expression vector has been integrated into chromosome ; vi ) the two incompatible lox sites, loxp and lox2272, would facilitate cre - recombinase mediated cassette exchange ( rmce ), which in theory will leading to develop a technology of site - specific gene expression in animal mammary glands
該載體的特點是：具有可以調控外源基因在乳腺中特異表達的牛-酪蛋白基因5 `端側翼區和包括第一外顯子及內含子在內的5 `端調控區；將人血清白蛋白cdna準確地置於牛-酪蛋白基因第二外顯子中的翻譯起始密碼子atg之後，而且沒有增加額外的序列和使人血清白蛋白cdna移碼；引入標記基因gfp ，便於在胚胎期鑒定陽性胚胎，減少受體；引入cre lox重組系統： （ ? ）標記基因gfp的兩端的兩個loxp位點可以在表達載體整合到基因組后，刪除標記基因； （ ? ）餘下的一個loxp位點可以和前面的lox2272位點組成cre重組酶介導的盒式交換系統。 -
By estimating and testing the recombination fraction, it searches the evidence of linkage between a known marker system and a putative gene for a disease
通過估計並檢驗重組率，連鎖分析尋找已知的標識系統和待推定的疾病基因座之間的連接證據。 -
One gene cassette usually contains a open reading frame encoding antibiotic resistance and a specific recombination site called attc ( or 59 - base element )
基因盒往往由一個編碼抗生素抗性的開放讀碼框（ orf ）和一個整合位點attc組成。 -
Currently, the research methods can be classified into two main classes, that is, genetic linkage analysis and linkage disequilibrium analy - sis. the two methods explore recombination fraction and linkage disequilibrium coefficient correspondly which are used to measure genetical characters, moreover, statistical methods are used to execute gene mapping
基因定位是人類基因組計劃（ hgp ）重要目標之一，目前研究方法主要分為兩大類：基因連鎖分析與連鎖不平衡分析。兩種方法分別藉助衡量遺傳性質的重組率與連鎖不平衡系數，並利用統計方法來進行基因定位。 -
The results were as following : 1. construction and identifcation of recombinant plant expression vector pbi ! 2i - th by dna recombination technology, sweet protein thaumatin gene was cloned into plant expression vector pbii2i. recombinant plasmid pbii2i - th was constructed successfully by enzyme cutting and electrophoresis
甜蛋白thaumatin基因植物表達質粒pbi _ （ 121 ） - th的構建與鑒定利用dna重組技術，將植物甜蛋白thaumatin基因克隆至植物表達載體pbi _ （ 121 ）中，通過酶切、電泳，鑒定thaumatin基因已成功構建到植物表達質粒pbi _ （ 121 ）中。
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