reference serum 中文意思是什麼
reference serum
解釋
標準血清-
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。 -
Three ibdv strains, designated as zj2000, zj991 and zj992, were isolated from bursa of fabricius of the sick chicken flocks in hangzhou, shenzhou, and ningbo of zhejiang province. the isolates could react with ibdv positive reference serum in the agar immune - diffusion test and caused 30 - 100 % mortality when passaged in chicken embryos. chickens inoculated with these isolates exhibited clinical and pathological signs typical of infectious bursal disease
從浙江省杭州、嵊州，寧波等地暴發疑似傳染性法氏囊病的雞群中採集法氏囊病料（編號分別為zj2000 、 zj991 、 zj992 ） ，分離病毒，經瓊脂免疫擴散試驗、動物回歸試驗、雞胚回歸試驗和電鏡觀察等證實所分離的3株野毒是傳染性法氏囊病病毒。 -
All staff are advised to keep their basal serum for future reference
勸諭所有工作人員備留基底血清，以作日後參考之用。 -
Milk, dried milk, buttermilk, powdered milk, serum and powdered serum. determination of phosphatasic action reference method
奶干奶脫脂奶和奶粉乳清和乳清粉.磷酸酶作用的測定 -
In this paper, three human dna fragments were coinjected into mice in order to study the influence of uncoding large dna fragment on hsa or hfen1 gene ' s integration efficiency, to explore methods to produce high expression of human serum album animals, to find out how to establish multi - transgenic animals in a way of saving time and money, to provide experience and reference to others. it mainly contains the following aspects : firstly, according to the sequence of human serum album gene cdna in genebank, one primer was designed
本實驗以小鼠為研究對象，採用兩種基因? hsacdna 、 hfen1和一種非編碼大片段dna進行共注射，旨在探索非編碼大片段dna對hsa和hfen1基因整合的影響及建立提高hsa基因整合效率的轉基因動物的方法，同時，探索多基因轉基因動物建立的策略，以便尋求一種既節省時間，而又簡單經濟的轉基因方法，為以後的研究提供一點的探索性經驗和參考。
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