refold 中文意思是什麼

refold 解釋
再折疊
  1. We have used two different operating schemes, fed - batch and step - addition, independently and collaboratively to refold denatured lysozymes

    本研究中，我們利用饋料式與間歇式不同的復性方法，以獨立地或結合地方式進行溶菌酶之復性。
  2. Mass spectrometry of synthetic hw - ma and rgd - hw are in full agreement with those speculated theoretically, which proves the success of peptide synthesis and refold. on isolated mouse phrenic nerve - diaphragm preparations, hw - ma can block the neuromuscular transmission in 35 minutes or so ( l 10 - 5 g / ml ), its biological activity shows 73 % decrease comparing with biological activity of native hwtx - i. it proves t hat the protein engineering of synthetic chimera hwtx - i has gained success to some extent, although it did not achieve our expectations. thus it proved that hwtx - i can be using as natural scaffold for protein engineering. and also emphasized the importance of " local stereo circumstances " of activity site when the foreign activity site was transferred into a natural scaffold

    濃度為1 / 1059 / ml的hw一ma突變體能可逆阻斷小白鼠隔神經書高肌的接頭傳遞，阻斷時一間為35min左右，與天然hwtx一i比較，生物學活性下降3一4倍，說明合成的突變體改造獲得了一定的成功，盡管與我們預期的目標有一定的差距，從而證明hwtx一i可以作為蛋白質工程研究的天然分子骨架，同時也強調了往天然分子骨架中轉移外源活性位點時維持活性位點「局部立體環境」的重要性。
  3. This paper describe the formation, isolation, denaturation and renaturation of recombinant proteins as inclusion bodies in e. coli, and summarize the most efficient ways to refold recombinant proteins

    摘要描述了大腸桿菌異源重組蛋白質的形成、制備、變性和復性，綜述了國內外變性、復性的有效方法。
  4. Refold along the crease, enclosing mixture, and make a double 1 / 2 - inch fold along the 3 open sides

    依照摺痕，再次折疊，包住食物，在其他三個開放面上也重復折疊兩次，每次1 / 2英寸。
  5. By the use of cirular dichroism and fluorescence techonologies to predict the secondary structure of protein, to determine the secondary structure of a protein, and to explore the unfold - refold mechanism of a protein

    運用圓二色譜和熒光技術分析預測蛋白質二級結構，研究蛋白質的穩定性與蛋白質的變性復性動力學機制。
  6. Section hi : purify & refold recombinant hpk - 5 protein was efficiently expressed in e. coli jm109 as inclusion bodies. after bacteria were smashed by ultrasound, te buffer, 1 % ttiton x - 100 and 2 m urea was used to efficiently extract inclusion bodies

    用含sm尿素溶液洗滌包涵體， 8000r / min轉速下分離包涵體，能最大限度去除雜蛋白，同時不會降低目的蛋白的損失。
  7. We used gradient dilution renaluration ( renaturation buffer : 0. 14mol / l tris - hcl ph8. 0. 10mmol / lgsh, 2mmol / lgssg. 5 % glycerol, 0. 02mol / l l - arg ) and gel filter renaturation by sephacral s200 to refold the inclusion body respectively. to get pure nk4, it was purified by gst affinity gromatochraphy. to detect the angiostatic activity of nk4 in vivo, it was tested using cam

    6 ）誘導溫度對pg廠x一4t一l一nk4表達菌株bl21表達目的蛋自的影響分別進行了25 』 c 、 300c 、 37溫度狀態卜，以iptg化學誘導，發現其均可表達，全菌表達量: 37最高，其次為30誘導時。
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