refolding 中文意思是什麼

refolding 解釋
重褶皺作用
  1. After the protein refolding of denaturant inclusion body following dialysis, we got the pure recombinant gst - eo protein by gst affinity columns. using the purified protein as coating antigen, an indirect elisa were developed for detecting the anti - eo antibody in the csfv serum by exploring the concentration of coating an tigen and dilution degree of serum

    使用分步透析法對變性的包涵體進行復性，將復性蛋白過gst親和層析柱得到純化的gst - eo融合蛋白。以gst - eo融合蛋白為診斷抗原，初步建立了用間接elisa檢測豬瘟血清eo抗體的方法。
  2. Subsequent refolding step was optimized for a maximal recovery of biological active protein. the antiangiogenic activity of the hpk - 5 was estimated using a chorioallantoic membrane ( cam ) model of chicken embryos

    4 .雞胚絨毛尿囊膜實驗結果顯示， hpk一5能有效的抑制雞胚絨毛尿囊膜新生毛細血管的生長。
  3. The results of lauryl sodium sulfate - polyacrylamide gel electrophoreses ( sds - page ) of the aggregate precipitate and supernatant and the result of high - performance size - exclusion chromatography of the supernatant indicated that, by wrongly linked intermolecular disulfide bonds soluble bi - molecular and tri - molecular egg white lysozyme aggregate could be simultaneously formed except being renatured to native and active egg white lysozymes during the refolding procedure of denatured - reduced egg white lysozyme ; the aggregate precipitate could be further formed by the non - covalent bonds interaction between the soluble hi - molecular egg white lysozyme aggregates, and the soluble tri - molecular egg white lysozyme aggregate could still stay at the supernatant

    沉澱和上清液的不連續十二烷基硫酸鈉聚丙烯酰胺凝膠電泳（ sds - page ）和高效凝膠排阻層析分析結果表明，還原脲變性蛋白溶菌酶在稀釋復性過程中除了能夠復性成天然態蛋白溶菌酶分子外，還會形成可溶的蛋白溶菌酶分子二聚體和三聚體，二聚體和三聚體主要是靠分子間二硫鍵的錯配連接而成的；可溶的蛋白溶菌酶分子二聚體之間通過非共價鍵相互作用而形成集聚體沉澱，而可溶的三聚體溶菌酶分子則仍處于復性液上清液中。
  4. To study the renatural condition of r hpf4, we adopted air oxygenation method to make r hpf4 refolding correctly

    研究重組hpf4的復性條件，採用空氣氧化法使重組hpf4正確重摺疊。
  5. The aggregate of egg white lysozyme molecules formed during the refolding procedure of denatured - reduced egg white lysozymes was analyzed by protein electrophoreses and high - performance size - exclusion chromatography

    本文利用蛋白電泳和高效凝膠排阻層析法分析了還原脈變性蛋白溶菌酶稀釋復性過程中的集聚體。
  6. Refolding and high - level expression study on human interferon - in e. coli

    在大腸桿菌中高效表達和色譜復性研究
  7. Expression and refolding of a hla - a 0203 - bsp fusion protein and identification of its tetramers

    的表達和復性及其四聚體的鑒定
  8. Various strategies, including the direct dilution method, have been utilized to enhance the performance of the refolding process ; however, refinement and improvement on these methods are ongoing

    直接稀釋法是復性程序中最簡單的方法，但如何提高其復性效率仍在持續研究中。
  9. Finally, a combined scheme was proposed to optimize the refolding process, this work demonstrates the feasibility of applying fed - batch and / or step - addition operation schemes to the renaturation of enzyme activity

    最後本研究中，結合饋料式與間歇式復性法之操作策略，可更有效率地提高其復性效率。
  10. Our research shows that the fed - batch and the step - addition schemes overcame the aggregation formation and favored the refolding step at higher feeding concentrations in comparison with the typical dilution method

    由實驗結果顯示，饋料式與間歇式復性法相較于稀釋法已有效地避免聚集體之生成及提高最終的操作濃度。
  11. Another chimera rgd - hw did not showed activity of platelet adhesion and aggregation that rgd sequence is thought to possess after complete refolding and purification, which explained that the resign and the progress of refolding of chimera rgd - hw was required improvement further

    另外一個突變體rgd一卜鉚經完全復性及分離純化后沒有顯示出rgd序列應具有的抗血小板聚集活性，說明這個突變體分子設計和氧化復性過程還有待進一步優化。
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