renaturation 中文意思是什麼

renaturation 解釋
蛋白質的復性
  1. Renaturation of thermolabile hemolysin inclusion bodies expressed by vibrio parahaemolyticus

    融合表達的副溶血弧菌不耐熱性溶血毒素包涵體復性的研究
  2. Expression of human fab antibody against keratin in e. coli and its renaturation

    抗體在大腸桿菌中的表達與復性研究
  3. We also synthesized w - ctx mviia and studied its renaturation under air oxidizing and gsh - gssg condition. we researched the physiological activities of the renatured peptides on isolated mouse phrenic nerve - diaphragm preparations or on isolated mouse spermaduct preparations

    另外還合成了- ctxmv a ,並用空氣氧化法和谷胱甘肽法,研究了多種條件下的復性情況。
  4. After renaturation, the biological activity of mviia is nearly 100 % of that of natural one ; the activity of hm - 1227 is less than t | iat of hwtx - i ; however, hm - xw cannot block the neuromuscul ar transmission in an isolated mouse phrenic nerve - diaphragm preparation

    復性后的mv a摘要生物學活性接近天然mvlla的100 % ;嵌合體hm一1227的活性小於hwtx一i的15 % ;而嵌合體fd以一xw不能阻斷小鼠隔神經一隔肌的接頭傳遞。
  5. Inhibition experiment of blood vessel proliferation in the chicken chorioallantoic membrane was used to study the biocharacter of r hpf4 and renaturation effect

    採用雞胚絨毛尿囊膜血管生成抑制實驗觀察重組hpf4的血管生成抑制活性及復性效果。
  6. At first, the systematic studies was made on the mechanisms and optimal conditions of denaturation and renaturation of mviia which lay the groundwork of renaturation of chimera of hwtx - i

    先利用合成的mviia為模式肽,系統的研究了此毒素的還原氧化復性機制和最佳的復性條件,為hwtx -嵌合體的氧化復性奠定基礎。
  7. After washing with reagent, adopt the newest purification technology source30rpc, sds - page and densitometric scan analysis, the result show that expression level is 90 % of total bacterial proteins. after renaturation, ifnr, hgfa, hgfb, hpk5 were purified by akta purifier chromatogram instrument, sepharose fast flow, ssphacrayl series gel, selecting optimize condition. finally establish a kind of high efficient purification model of recombinant proteins produced in escherichia coli as inclusion bodies, purification product purity > 98 %

    結論:總之,通過對發酵罐中重組工程菌各種培養因素的研究,建立了一種高密度、高表達發酵工藝體系,為重組蛋白的后續純化提供了大量、穩定的原料供應;通過對不同目的蛋白的色譜行為的系統研究,建立了一種高效穩定、快速簡潔、易於放大的包涵體重組蛋白分離純化體系。
  8. Purification and renaturation of recombinant human cu, zn - sod by metal - chelating affinity chromatography

    金屬螯合親和層析純化和復性重組人銅鋅超氧化物歧化酶
  9. This paper describe the formation, isolation, denaturation and renaturation of recombinant proteins as inclusion bodies in e. coli, and summarize the most efficient ways to refold recombinant proteins

    摘要描述了大腸桿菌異源重組蛋白質的形成、制備、變性和復性,綜述了國內外變性、復性的有效方法。
  10. The research of the renaturation and biological activity of the chimeras confirmed the active sites of hwtx - i and mviia further. it also indicates that the structure of ick is stable and is an ideal model molecule for protein engineering

    通過對嵌合體復性和活性情況的研究,進一步證實了hwrtx一i和mvlla的活性位點;並且表明抑制劑朧氨酸結模體( ick )的結構確實是相當穩定的,是一種進行蛋白質工程的較理想的模型
  11. Finally, a combined scheme was proposed to optimize the refolding process, this work demonstrates the feasibility of applying fed - batch and / or step - addition operation schemes to the renaturation of enzyme activity

    最後本研究中,結合饋料式與間歇式復性法之操作策略,可更有效率地提高其復性效率。
  12. Then after purification, renaturation, cleavage and ultrafiltration centrifugation, igf - 1 and des ( l - 3 ) igf - l protein were obtained

    Westernblot結果表明iptg誘導表達產物可與特異性igf - 1抗體發生反應。
  13. We used gradient dilution renaluration ( renaturation buffer : 0. 14mol / l tris - hcl ph8. 0. 10mmol / lgsh, 2mmol / lgssg. 5 % glycerol, 0. 02mol / l l - arg ) and gel filter renaturation by sephacral s200 to refold the inclusion body respectively. to get pure nk4, it was purified by gst affinity gromatochraphy. to detect the angiostatic activity of nk4 in vivo, it was tested using cam

    6 )誘導溫度對pg廠x一4t一l一nk4表達菌株bl21表達目的蛋自的影響分別進行了25 』 c 、 300c 、 37溫度狀態卜,以iptg化學誘導,發現其均可表達,全菌表達量: 37最高,其次為30誘導時。
  14. Ii ) the renaturation of synthetic hpab was carried out in glutathione oxidation / reduction solution ( l : 10 ). the renatured synthetic hpab could kill escherichia coli, pseudomonas aeruginosa and staphylococcus aureus on lb agar plates. thirdly, construction of artificial mutants of hpab

    3 .突變體的設計及其重組桿狀病毒構建:採用同源模建法分析hpab一p的空間結構,並以此結構為基礎,設計hpab一p突變體hpab一p38和hpab一p34 。
  15. Expression, purification and renaturation of her - 2 neu rld protein

    純化及復性研究
  16. Cloning, expression, renaturation and purification of soluble hscf

    復性與純化
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