replication protein a 中文意思是什麼

replication protein a 解釋
復制蛋白a
  • replication : n 1 （尤指對答辯的）回答；【法律】（原告對被告抗辯的）答辯。2 （繪畫等的）復制，摹寫；拷貝，復製...
  • protein : n. 【化學】朊，蛋白（質）。
  • a : an 用在以母音音素開始的詞前〉 indefinite art 1 〈普通可數名詞第一次提到時，冠以不定冠詞主要表示類...
  1. A small cryptical plasmid pefr was isolated from enterococcus faecium strain df101. the complete sequence analysis of the plasmid show that it consists of 3176 bps, which contains four putative orfs. orf1 encodes a putative protein and is highly similar to repa which functions in replication

    從屎腸球菌df101菌株中分離到隱秘的小質粒pefr ，全序列分析顯示質粒pefr由3176bp組成，編碼四個推定的orf ， orf1編碼的一個推定的蛋白和復制有關的repa有很高的相似性。
  2. In e coli, dna polymerases are key enzymes involved in two distinguished pathways contributing to untargeted mutagenesis. replication of dna by pol v ( umuc ), in the presence of umud1, reca and single strand binding protein ( ssb ), is highly mutagenic and exhibits a predominant mutation pattern of base transversion. another error prone polymerase involved in untargeted mutagenesis is pol iv, encoded by dinb gene

    在umud ' ， reca和單鏈結合蛋白ssb的協助下， polv （ umuc ）能在單鏈模板上催化dna合成並產生高頻率的以堿基顛換為主要形式的突變；另一個與非定標性突變有關的易誤dna聚合酶是pol ，為dinb基因的編碼產物。
  3. The orf1 proteins of all four chinese senv isolates had two motifs ( ftl and yxxk ) which were conserved in replication - associated proteins. in all four chinese senv isolates, the putative non - structure orf2 protein had a cav - like region. the putative orf3 protein had a serine - rich tract preceded by a cluster of basic amino acids ( r and k ). database scaning suggested this region had high a percentage of homology with dna topoisomerase i protein of d. melanogaster, and might play an important role in the replication of such single - stranded dna viruses

    所有這四個中國分離株，均保留了o即1中與復制有關的保守序列（ ftl和yxxk ） :在推測是非結構蛋白的orfz中均含有cav樣保守區w 『 x7一h一x3一c一xl一c一xs一h ; orf3蛋白的堿性氨基酸（精氨酸和賴氨酸）簇后緊接著一個富含絲氨酸的區域，與d . molanogaster的dna拓撲異構酶i有一定的同源性，推測在單鏈dna的復制中起作用。
  4. A bt - e. coli shuttle vector pht315 was deleted its replication region of bt, then constructed a novel vector named pht315 - 1 which composed a multiple cloning site, erythromycin and ampicillin - resistance marker and could only replicated in e. coli. used pht315 - 1, a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324. sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501, 333, 183aas. orfl had 98 % identities with replicating related protein ori43 of bt strain hd263. the others were no homology to any published bt replicating related protein. after continuous cultured for 70h at 30 c without antibiotic selecting press. the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 %. and growth curve also showed that the novel replicon was stable and could replicate normally

    進一步序列分析表明該復制區至少有3個較大的orf ，分別編碼501 ， 333 ， 183個氨基酸。其中orf1蛋白序列與hd263復制蛋白ori43的同源性為98 ，而另外兩個orf和genbank己公布的bt復制相關蛋白無同源性。 30連續培養72h ，復制區質粒在bt無晶體突變株hd73cry ~ -中穩定性達98以上， 30h生長曲線也表明該復制區能夠在bt中穩定復制和遺傳，對受體菌株無明顯不良影響。
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