restriction allele 中文意思是什麼
restriction allele
解釋
限制酶基因- restriction : n. 1. 限制，限定。2. 拘束，束縛；自製。3. 【邏輯學】限定。
- allele : n. 【遺】等位基因，對偶基因。
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To construct eukaryotic expression vector of mbl gene with codon 54 point mutation, the target sequence in pgem - mbld plasmid, which conains mbl cdna with codon 54 mutant allele, was amplified by pcr. after the cdna fragement and plasmids pci - neo were prepared by digestion with sma i and sal i, the fragment was inserted into sma i and sal i site in pci - neo eukaryotic expression vector, and the recombinant vector, named pci - mbl54, was obtained. the pci - mbl54, digested with restriction enzymes, was found to contain the point mutation mbl cdna by agarose gel electrophoresis analysis
本實驗以ggc54gacmbl突變為研究對象，選用真核表達質粒pci - neo ，根據已構建好的含54位密碼子突變型mbl基因t載體的結構，設計合成新的引物， pcr擴增54位密碼突變型mbl基因，凝膠回收，雙酶切pcr產物和pci - neo質粒， t4連接酶連接，將前者克隆至後者的sma和sal位點，轉化大腸桿菌xl - 1blue ，氨芐選擇培養。
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