restriction site pcr 中文意思是什麼

restriction site pcr 解釋
限制性位點pcr
  • restriction : n. 1. 限制，限定。2. 拘束，束縛；自製。3. 【邏輯學】限定。
  • site : n 1 地點；位置；地基。2 場所，現場。3 遺址。4 【計算機】網站，站點〈電腦網路用戶的網站地址〉；萬...
  • pcr : PCR ＝polymerase chain reaction 【生物化學】聚合酶聯鎖反應〈此項技術可用以復制犯罪現場發現的DNA小樣，從而有助於破案〉。
  1. The hinf i restriction enzyme digestion gave rise to restriction fragment length polymorphism ( rflp ) of the pcr products. when there were two dna fragments of 363bp and 305bp were produced from a pcr product, the strains were assumed to have a mutation at ser83, when three fragments of 363bp, 206bp and 99bp were produced, the strains were assumed to have no mutation at the 83or 116. the results indicated that certain mutation of ser 83 abolished a hinf i restriction enzyme site and may be detected as a rflp

    本研究重點分析了16株菌對氟喹諾酮類（ fqns ）藥物的耐藥性，結果表明： 16株菌對諾氟沙星、環丙沙星、沙拉沙星、單諾沙星和氧氟沙星等（ nor 、 cip 、 sar 、 dan 、 ofl ）的耐藥率在31 . 3 - 56 . 3之間。
  2. 2. ecori / bamhi digested pcr products were inserted into the corresponding restriction site in the expression plasmid pbv220. construction of non - fusion expression plasmid pbv220 - endostatin

    用ecori和bamhi酶切endostatincdna的pcr產物，將其插入到質粒載體pbv220中相應的限制性酶切位點，構建非融合質粒表達載體pbv220 - endostatin 。
  3. To construct eukaryotic expression vector of mbl gene with codon 54 point mutation, the target sequence in pgem - mbld plasmid, which conains mbl cdna with codon 54 mutant allele, was amplified by pcr. after the cdna fragement and plasmids pci - neo were prepared by digestion with sma i and sal i, the fragment was inserted into sma i and sal i site in pci - neo eukaryotic expression vector, and the recombinant vector, named pci - mbl54, was obtained. the pci - mbl54, digested with restriction enzymes, was found to contain the point mutation mbl cdna by agarose gel electrophoresis analysis

    本實驗以ggc54gacmbl突變為研究對象，選用真核表達質粒pci - neo ，根據已構建好的含54位密碼子突變型mbl基因t載體的結構，設計合成新的引物， pcr擴增54位密碼突變型mbl基因，凝膠回收，雙酶切pcr產物和pci - neo質粒， t4連接酶連接，將前者克隆至後者的sma和sal位點，轉化大腸桿菌xl - 1blue ，氨芐選擇培養。
  4. Genes coding mature peptide of igfs were achieved by pcr using another pair of oligo - nucleotide primers to induce to the suitable restriction enzyme site, and the igf - i product of pcr contains 230 base pairs. igf - ii contains 219 base pairs. 3

    各另外設計一對特異性pcr引物，導入適當限制性內切酶切點，以上述連有目的基因的克隆載體為模板，採用pcr方法擴增基因片段，獲得長度約230bp的igf -和219bp的igf -成熟肽基因序列。
  5. All the subjects were genotyped by pcr - rflp ( polymerase chain reaction - restriction fragment length polymorphism ) at polymorphic sac i site inside the exon 7 of the ahsg gene. this polymorphism involves a nucleotide substitution of c to g at the middle nucleotide of the codon at amino acid position 238 resulting in the replacement of threonine ( acc ) with serine ( agc )

    所有的樣本通過聚合酶鏈式反應?限制性片段長度多態性方法（ pcr - rflp ）對ahsg基因的第7個外顯子內的sac多態性位點進行基因分型，該多態性位點為238號氨基酸密碼子中間的堿基c到g的替換，使蘇氨酸（ thr ， acc ）變為絲氨酸（ ser ， agc ） 。
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