reverse strain 中文意思是什麼
reverse strain
解釋
反向形變-
A pair of primers were chemically synthesized based on the cdna sequence of hog cholera virus strain brescia and used to amplify the cdna fragments of envelope glycoprotein e2 gene of hclv which coding the major protective antigen by reverse transcription - polymerase chain reaction ( rt - pcr ) from total intact rna extracted from infected calf testicle cell culture by hclv
以豬瘟兔化弱毒犢牛睪丸細胞毒（ hclv ）中提取的細胞總rna為模板，以rt - pcr技術，擴增出了完整e2基因的cdna片段。經電泳、酶切分析，證實了所擴增片段為e2基因特異性片段。 -
G gene of rabies virus m, the of two main regions ( about 1000nt ), ranging from 3161nt to 4162nt and ranging from 4012nt to 4863nt of glycoprotein gene of rabies virus strain m, isolated from mouse in he nan, china were amplified by reverse transcriptase - polynerase chain reaction ( rt - pcr ) in order to complete glycoprotein gene of strain m. these regions were sequenced by the produce of pcr directly. comparison and analysis of nucleotide sequence and amino acid sequence deduced with that of other strains published was performed by computer with dnasisv 2. 5demo software
本研究對我國河南某地野鼠體內分離的狂犬病野毒株mrv基因的3161位? 4162位（ 1001個堿基）和4012位? 4863位（ 851個堿基）片段進行了反轉錄pcr擴增和序列測定，得到mrv的糖蛋白基因全序列，用dnasisv2 . 5demo分析軟體，與已發表的代表性毒株g基因全序列進行核苷酸和氨基酸序列的比較分析，結果表明在同一基因型中， mrv和國際標準攻毒株cvs的同源性最高（ 96 . 5 ） ，和中國減毒株ctn的同源性最低（ 79 . 8 ） 。 -
The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。 -
Researching on the technology if reverse order is to study strains if pits, structural systems if basement, environmental surwey and underpinnings. on account of emphasizing developing methods if checking strength if pick - ets, modemizing machenes of excavation and studying methods of underpinning is put forward and is a way if controlling the quality of pickets in sites, which leads an active effect ; synthetic application if rankintheory, spatial and time effect theory to excavation tl aanalyze the state of soil force and strain is brought forward and the time effect should be considered in the zone of clay, the formation and development of soil plasticity are analyzed and the most dangerous zone to decide how to excavate and where to begin is found ; analyzing the cause of picket settlement during reverse order and the differential settlement and discussing hlw to solute it. duringh the temporary survey and the environmental warship, bringing rorward the theory of environmental vibration and analyzing the state of soil force and probability of losing stabilization of soil under the effect of environmental vibration ; analyzing the state offeree in underground concrete wall by the method of mathematics and pointing out the place of the maximum force and deformation. based on systematic illustrating the reverse order, problems about application and development of reverse order and suggestions also are expressed
鑒于國內外的研究把重點放在大力發展工程樁的實驗室承載力監測方法與設備、如何使土方開挖機械現代化及對周圍建築的臨測方法上，本文提出了現場利用聲波層析成像技術監測鋼砼樁內部質量的方法與程序，並得出了聲波層析成像技術是砼樁的動態質量檢測的有效手段，這對指導施有積極、現實意義；提出了綜合運用朗肯土壓力理論、基坑空間和時間效應影響理論來分析逆作法施工過程中基坑邊坡土體應力及應變的變化情況，指出粘土地區也應考慮時間效應，並且進一步分析了基坑邊坡土體的塑性區形成和發展，找出邊坡最不利的區域，以確定地下室土體的挖掘的方式和順序，指出憑主觀臆斷與經驗來施工是不可取的；在分析、經較逆作法與大開挖順作法的地下室結構體系受力情況及施工順序的不同，提出了節點處理技術；分析了逆作法施工期間樁的沉降變化原因及由此而產生的差異，並探討了解決的方法；本文還提出了環境振動對土體邊坡穩定產生影響的觀點，並分析了在環境振動影響下，土體的應力狀態及土休失穩破壞概率，並且還運用彈性力學知識和數學分析的方法定量地分析了地下混凝土墻受力狀態，指出了被監測墻體的最大應力、應變位置。 -
5 ) as the deformation strain increases the reverse transformation temperature and thermal hystersis of tini film increase on the first heating, while the martensitic transformation temperature decreases and r phase transition temperature is constant
隨預應變量增大， tint薄膜的第一次逆相變溫度升高，相變熱滯增大，馬氏體相變溫度降低， r相變溫度無明顯變化。 -
The simulation of hemisphere bulging shows that the thin rate decreases about 10 % with direct - reverse process. cavity rate is decreased evidently in the bulging if back pressure is provided. experiment shows that proper back pressure can eliminate big cavity on the condition that strain is not very high
通過增加脹形背壓，降低成形件的空洞率，結果顯示背壓對于空洞率的降低有比較明顯的作用，適當的背壓甚至可以基本消除應變不是特別大時的空洞。 -
These results showed that the three isolates were infectious bursal disease virus. the full - length cdna of the genomic segment a of two viruses, one virulent field strain ibdv zj2000 and one attenuated strains ibdv jd1, were amplified in a single step procedure by long - accurate reverse - transcription polymerase chain reaction ( la - pcr ), cloned into pgem - t easy vector, and sequenced
分別以傳染性法氏囊病病毒zj2000株（野毒株）和jd1株（弱毒株）基因組dsrna為模板，採用long - accuratert - pcr （ la - pcr ）一步法擴增並克隆了兩株病毒的基因組a節段全長cdna 。 -
The special primers were designed and synthesized after the analysis to the nucleotide sequence homology of etrl recorded in genbank. the total rna extracted from latex was reverse - transcribed into first strain of cdna. the special amplification products ( cdna fragment ) were obtained from pcr when using the first strain of cdna as template
通過對genbank登錄的已克隆的植物etr1基因序列進行核苷酸同源性分析，設計特異引物。以膠乳總rna為模板，反轉錄成cdna第一鏈，又以cdna第一鏈為模板進行pcr擴增，得到特異擴增片段。
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