rhodococcus equi 中文意思是什麼
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Therefore, research on cholesterol oxidase has become more and more important. a 1. 6 kb dna fragment was amplified using pcr method from genomic dna of rhodococcus equi. 4 - 2g2
本研究採用pcr方法從分泌膽固醇氧化酶的馬紅球菌菌株4 ? 2g2基因組dna中，擴增出特異dna片段，該擴增片段全長約1 . 6kb 。 -
Sequence analysis showed that, this fragment has a homology of 99 % to the previously reported choe from rhodococcus equi. after cloning and subcloning and three identical fragments were obtained from three independent pcr, lacking three continual bases ( ttc, encoding the pheamino acid ) compared with choe, thus it can be assumed that the new fragment, designated as choew, and choe belong to the same gene family, even it might be the natural mutation of choe
對片段進行克隆、亞克隆之後測序分析，發現與已克隆的來源於馬紅球菌的膽固醇氧化酶基因cboe有99的同源性；並且三次獨立的pcr均得到相同的片段，所克隆的基因序列與choe相比，連續缺失三個堿基（ ttc ） ，即相應的氨基酸序列缺失一個氨基酸（ phe ） ，因此判斷所克隆的基因片段與choe屬同一基因家族或原有基因的天然突變體，命名為choew 。 -
In this study, a new gene c / wew, encoding cholesterol oxidase, was isolated from rhodococcus equi. 4 - 2g2 found in china, which may be useful in clinical diagnosis healthy food and pest management in agriculture. in addition, the gene has been expressed successfully, the expression product has cholesterol oxidase activity, thus this work provided theoretical basis to the development of genetic engineering of cholesterol oxidase
本研究從我國自行分離的馬紅球菌4 ? 2g2菌株中分離到一種編碼膽固醇氧化酶的新基因choew ，它將可應用於臨床檢測、保健食品和農業抗蟲等領域；同時利用原核表達載體成功的在大腸桿菌中表達了目的基因，表現出膽固醇氧化酶酶活，為膽固醇氧化酶的基因工程利用開發作了一定的理論實驗基礎研究。
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