rna blotting 中文意思是什麼
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In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。 -
Total rna samples used in northern blotting were extracted from the light - exposed and light - interrupted cortex tissue of tuberous root of the two turnip cultivars
Northern分析採用的rna樣品，是從上述兩品種的光處理和暗處理的肉質根部周皮層中提取的總rna 。 -
Dna and rna dot blotting revealed that the f gene was transcribed into mrna in the vero cells. there was expression of the f protein as shown by indirect immunofluorescent assay. the expression began at 48h post - infection and increased thereafter, as indicated by elisa
將真核表達質粒pcdna3 - f高壓電轉化dam和phop基因雙突變的減毒鼠傷寒沙門氏菌zj111株（ zj111 / pcdan3 - f ） ，並直接轉染vero細胞，分別提取細胞總dna和總rna ， dig標記探針均可檢測到陽性雜交信號。 -
Expression analysis the expression analysis of gharf in different tissues of cotton was carried out by rna dot blotting with ghakf probe labeled with dig. the result showed that the expression of arf gene was mainly in anthers of sterile and fertile, pollen of fertile, corolla rather than in leaves, radicel and ovule
棉花ari ； 』基因的表達分析用地高辛將棉花arf基因標記為探針，與棉花洞a的不育株花藥、司有株花藥、可育花粉粒、葉片、花冠、胚根和胚珠的總rna進行斑點雜交。 -
The dna and rna dot blotting revealed transcription of the polyprotein gene into mrna in the vero cell
比較了dna疫苗和兩種常用的弱毒苗（ b87 、 d78 ）的安全性和免疫效力。
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