rt-nested pcr 中文意思是什麼

rt-nested pcr 解釋
逆轉錄-套式聚合酶鏈反應
  • rt : 1. radiotelegraphy 無線電報術。2. radiotelephony 無線電話術。
  • nested : 巢狀的
  • pcr : PCR =polymerase chain reaction 【生物化學】聚合酶聯鎖反應〈此項技術可用以復制犯罪現場發現的DNA小樣,從而有助於破案〉。
  1. In order to identifiy the virus further, a set of double nested primers for canine coronavirus was selected. the primers were designed in s gene region from ccv including two pairs of primers : ccvfl - ccvrl, ccvf2 - ccvr2. the first is a pair of outer primer, and can amplify a fragement of 1086bp. the second is a pair of inner primer. and can amplify a fragement of 515bp. using the nested primers, many ccv strains can be identificated including k378, insave - l, ccv 1 - 71 etc. synthesizing this set of primers, we selected the panda ' s liver - tissue materials and some different passages of viral culture to amplify by rt - pcr, and all of them respectively gained two target fragements of 1086bp and 515bp, but the control cell did not

    合成該套式引物,選擇大熊貓原代病料和病毒各代細胞培養物,經套式( nested ) rt一pcr擴增,可得到一與設計值5巧bp相符的dna片段,經bst一xl ( 590 , 1110 )酶切鑒定,證明該擴增片斷為特異性片段;回收大熊貓肝組織原代病料和細胞培養物第2 、 3 、 29代的ccvfz一ccvrz擴增片段,純化,送生物公司測序。
  2. It indicated that developed primary rt - pcr and nested - pcr are rapid diagnostic method for ibdv

    為ibd臨床快速診斷提供了一種有用而可靠的新技術。
  3. 34 field samples, which were collected from different places of guangxi during 1993 to 2003, were detected by using developed primary rt - pcr and nested - pcr

    結果基礎rt - pcr方法檢測到其中11份病料為陽性, nested - pcr則檢測到23份陽性。
  4. Methods : extracting the total rna of human pbmc, the objective include 60 healthy blood donator, 30 patient with viral encephalitis and multiple sclerosis and parkinsonian syndrome, 30 patient with schizophrenia and affective disorder, this indviuals were inpatients or outpatients of the first hospital of chongqing university of medical science from december, 2000 to june, 2001. using nested rt - pcr techique to detect borna disease virus " middle fragment in orf i, and using southern blot hybridization to analyze the pcr product

    重慶醫科大學碩士學位論文方法:提取從2000年12月至2001年6月在重慶醫科大學第一附屬醫院神經科和精神科住院及門診的60例健康獻血者、 30例包括原因未明的病毒性腦炎、多發性硬化、帕金森綜合征,以及30例包括精神分裂癥和情感障礙患者pbmc中的總kn 』 a ,採用套式逆轉錄聚合酶鏈反應estedrticr )技術進行了bdvorf基因中部片段的檢測,並對pcr產物進行電泳分析及southernblot雜交分析。
  5. It has made the strong basis for further studying mechanism of replication, virulence and determinant, attenuation, pathogenesis, functions of genetic products, specific diagnosis, cell and host tropism, development of dna vaccine and marker vaccine of csfv, and provided an excellent tool for molecular virology. main research contents include : based on published nucleotide sequences of csfv and by the help of computer analysis software, high conservative regions and single restriction enzyme sites of genome were selected. utilizing rt - pcr and nested - pcr techniques, 7 overlapping cdna fragments covering the full genome of csfv c - strain were successfully amplified

    中國豬瘟兔化毒(脾淋毒)基因組cdna文庫的構建、序列分析:根據已發表的豬瘟病毒( csfv )核苷酸序列,藉助計算機軟體分析,選擇高保守區段和基因組中的單一限制性酶切位點,利用rt - pcr及nested - pcr和helf - nestedpcr技術,成功地擴增出了覆蓋c -株全基因組的7個cdna重疊片段f1 f7 ,分別克隆到pmd - 18t或pgem - teasy載體進行測序后,拼接出了其核苷酸序列。
  6. But the primers designed for vvibdv can only amplify vvibdv strains. so we can typing strains between vvibdv and cibdv by using vvibdv - specific primers by one reaction. typing the pathotype of field ibdv in guangxi, 23 field sample " fragments were amplified by the primary rt - pcr or nested - pcr. they were cleaved with sspl and sacl

    第三,應用本研究建立的rea和vvibdv型特異性rtpcr技術,對34份采自廣西不同地區的ibdv病料,其中pcr反應陽性的23份的pcr產物,進行酶切分型和特異性引物擴增。
  7. Firstly, the eo genes of csfv - jl and csfv - ln9912 strains were amplified by rt - pcr and nested pcr and then sequenced after cloned into t easy vector. comparing the eo sequences with other strains eo by biosoftwares, dnastar5. 0 and bioedit, the phylogenetic analyse revealed that all of the strains we have sequenced could be divided into groupl and groupii. two amino acid streches of 15 csfv strains eo, which form the rnase active site, and histidine residues essential for rnase catalysis in both ones were highly conserved. the eo protein propertys of antigen epitope, hydrophobicity, isoelectric point were also predicted by bioinformatic method

    首先,採用rt - pcr和nest - pcr技術擴增了csfv - jl株和csfv - ln9912株eo基因,插入teasy載體后測序,應用dnastar5 . 0和bioedit軟體將其與本實驗室已測其它毒株和genebank中登錄的csfv代表毒株eo基因序列進行比較,繪制了遺傳進化樹並預測了eo蛋白的抗原表位、疏水性、等電點等特性。
  8. For the products of primary rt - pcr and nested - pcr are all encompassing the hyper - variable region of vp2 gene, so we can take a restriction enzyme analysis ( rea ) directly

    第二,本研究的基礎rt - pcr和nested - pcr所擴增的片段均是橫跨ibdv的vvp2的,所以,可直接對pcr產物進行酶切分型研究。
  9. Infectious bursal disease virus has been a great concern for the poultry industry for a long time, particularly for the past decade when its " re - emergence " in variant or highly virulent forms. in this study, two sets of primers ( pta and pts, ibda and ibds ), flanking the hyper - variable region of vp2 gene, were designed to run a reverse transcription polymerase chain reaction ( primary rt - pcr ) and nested - pcr. both of these assays can amplify all of 12 reference strains which including pathotypes cibdv, vvibdv and vibdv, but not the 5 negative reference pathogens of chicken

    結果12個參考毒株均能擴增出約679bp的目的片段,而陰性對照的常見5種雞病病原:雞新城疫病毒( ndv ) 、雞傳染性支氣管炎病毒( ibv ) 、雞傳染性貧血病毒( caiv ) 、大腸桿菌和多殺性巴氏桿菌均沒有擴增到任何片段;應用建立的技術對疑似ibd的34份臨床病料進行檢測,並同時在基礎rt - pcr擴增的片段內設計另一對引物進行nested - pcr 。
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