running gel 中文意思是什麼
running gel
解釋
電泳膠-
The probe detection and pcr amplification are contemporary, without ethidium bromide staining and gel running and any post - pcr manipulations, so that the contamination risks are reduced. those two methods were used to detect successfully the primus necrotic ringspot virus from sample delivered by beijing airport quarantine bureau. the amplified fragment was cloned into the vector of pmd18 - t then sequenced
我們利用建立的雜交誘捕們pcr elisa和隊f pcr對北京機場檢疫局送檢的櫻桃樣品檢出了pnrsv ，同時把451hp的pcr產物克隆到ppo18 t載體上，通過序列測定，同源世最高的是genebank中pnrsv資料序號gi15750502gb山57046 -
Dig labeled probe hybridization with solid pcr product was performed as well as electrophoresis of liquid product, this method combines rna purification, pcr amplification and nucleotide probe hybridization detection together and has many advantages including better rna purity, less time consumption, reliable positive reaction and 10 times sensitivity as rt - pcr gel running detection, reduce false positive result, unpurified nucleotide requirement, loug infected plant organism can be detected by solid hybridization
2 ）結果可靠，雜交特異性誘捕目的片段，同時去除了核酸中存在的pcr抑制物質，減少了因核酸提取不純造成的漏檢現象，結果輸出通過雙重判定保證了結果的可靠性。 3 ）靈敏度高，通過雜交進行結果判定，靈敏度比傳統的rt干cr大約高10倍。
分享友人