scatchard 中文意思是什麼
scatchard
解釋
斯卡查德-
The determination of human thymidine kinase ( htk ) in human serum, which is a key indicator of cancers can give information for the diagnosis and treatment of the malign diseases. the protein a layer was first self - assembled onto the gold electrode surfaces of quartz crystals, the monoclonal antibodies were then orientedly immobilized through the specific binding between the fc terminals of the antibodies and the self - assembled protein a. with this sensor, the affinity constant of antigen - antibody binding was estimated to be 1. 85 106 l / mol according to the scatchard ’ s plotting method, which proved the high bioactivity of antibody. finally, an amplified piezoelectric immunosensor was designed to determine the htk in
實驗中將蛋白a吸附於鍍金壓電石英晶體電極表面，用於定向固定htk單克隆抗體，成功研製了檢測htk的壓電石英晶體傳感器，並基於標準scatchard繪圖法，計算出免疫反應的親和常數為1 . 85 106l / mol ，證明該單克隆抗體具有較高的免疫活性;同時基於酶催化沉澱技術，設計了的檢測htk的質量放大壓電石英晶體傳感器，該傳感器可在0 . 1 - 10ng范圍內對htk進行定量檢測，應用此傳感器成功地對5種癌癥病人血清中htk的濃度進行了測定，實驗結果為癌癥的臨床診斷與治療提供了參考。 -
Part i this paper has minutely studied the interaction between ag ( i ) and serum albumin. the binding of ag ( i ) to human serum albumin ( hsa ) or bovine serum albumin ( bsa ) has been studied by equilibrium dialysis at ph ( 5. 4 ). the scatchard analysis indicates that there exists several strong binding sites of ag ( i ) in both hsa and bsa. a notable hysteretic effect has been observed in the interaction of ag ( i ) with hsa or bsa using uv - visible spectrometry at ph ( 5. 4 ), which shows that the binding between ag ( i ) with hsa or bsa may induce a slow transition of hsa or bsa from the conformation of weaker affinity for ag ( i ) to one of stronger affinity ( a - b transition ). the rate constants and activation parameters of this transition parameters of this tansition were measured and discussed. the binding equilibrium has been also studied by resonance light - scattering spectrum ( rls ) and flurescence quenching
第一部分：等離子點ph （ 5 . 4 ）條件下，用平衡透析法和紫外光譜，熒光光譜，共振散射光譜研究了ag （ ）與人血清白蛋白（ humanserumalbumin ，簡稱hsa ）或牛血清白蛋白（ bovineserumalbumin ，簡稱bsa ）的結合。 scatchard圖分析表明， ag （ ）在hsa或bsa中有強弱兩類結合部位，通過計算機擬合獲得結合的逐級穩定常數值。紫外掃描發現ag （ ）與hsa或bsa的結合存在滯後效應，表明ag （ ）與hsa或bsa的結合可能誘導蛋白質構象發生緩慢變化（ a - b ） ，測得並討論了這一構象變化的速度常數和活化參數。 -
Part ii this paper has minutely studied the interaction between au ( iii ) and serum albumin. the binding of au ( iii ) to human serum albumin ( hsa ) or bovine serum albumin ( bsa ) has been studied by equilibrium dialysis at ph ( 7. 43 + 0. 02 ). the scatchard analysis indicates that there exists several strong binding sites of au ( iii ) in both hsa and bsa. a notable hysteretic effect has been observed in the interaction of au ( iii ) with hsa or bsa using uv - visible spectrometry at ph ( 7. 43 + 0. 02 ), which shows that the binding between au ( iii ) with hsa or bsa may induce a slow transition of hsa or bsa from the conformation of weaker affinity for au ( iii ) to one of stronger affinity ( a - b transition ). the rate constants and activation parameters of this transition parameters of this tansition were measured and discussed. the binding equilibrium has project supported by the national science foundation of china ( no. 299621001 ), and the foundation for talents of the ten hunred thousand in guangxi
第二部分：生理ph （ 7 . 43 0 . 02 ）條件下，用平衡透析法和紫外光譜，熒光光譜研究了金（ ）與人血清白蛋白（ humanserumalbumin ，簡稱hsa ）或牛血清白蛋白（ bovineserumalbumin ，簡稱bsa ）的結合。 scatchard圖分析表明，金（ ）在hsa或bsa中有強弱兩類結合部位，通過計算機擬合獲得結合的逐級穩定常數值。利用熒光猝滅法計算出金（ ） - hsa和金（ ） - bsa體系的stem - volmer常數和雙分子猝滅速率常數。 -
The internalization of oligochitosan in macrophage is a time - course process. 2. initial experiments tested time, temperature, and concentration dependence of 2 - amac - oligochitosan uptake in macrophages. scatchard analysis of internalization of 2 - aminoacridone - oligochitosan in macrophages indicated its internalization is mediated by a specific receptor on macrophage membrane with ka of 2. 1 10 - 5m
2 . 2一氨基叮睫酮標記的殼寡糖與巨噬細胞的結合是一個依賴時間、溫度和濃度的過程， scatchard分析后發現它對巨噬細胞的作用是通過膜上特異性的受體（ kd為2 . 1x10一sm ）進行的。
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