selective gene 中文意思是什麼
selective gene
解釋
選擇基因-
A genetic transformation model for the brown seaweed undaria pinnatifida has been primarily set up by using micro - particle bombardment as the method, female or male gametophytes as the gene recipients, hybridization as the regeneration route and chloramphenicol, hygromycin or basta as selective reagents
本文從轉化受體、轉化方法、報告基因、再生途徑、篩選方法等方面對裙帶菜的遺傳轉化進行了研究。首先，分離並建立了裙帶菜雌雄配子體的無性繁殖系，進行了裙帶菜的不同再生途徑的研究。 -
By using primers designated for lacz gene, pcr result suggested an integration of lacz into u. pinnatifida genome. nine reagents including penicillin g, kanamycin, g418, teicoplanin, zeocin, chloramphinicol, hygromycin, basta and methotrexate were tested as selective reagents for selection of transgenic sporophytes. the results showed that young sporophytes of u. pinnatifida were sensitive to chloramphinicol and hygromycin and very sensitive to basta which suggest the potential of using the resistance genes as selectable markers
，為了解決轉基因裙帶菜的篩選問題，進行了裙帶菜幼孢子體對不同篩選壓力的敏感性實驗，包括抗生素類的青霉素g 、卡那黴素、 g418 、硫酸鏈黴素、鹽酸潔黴素、 zeocin 、氯黴素、潮黴素，除草劑類的basta ，氨甲喋呤類的氨甲喋呤，結果顯示裙帶菜幼孢子體對氯黴素、潮黴素敏感，對basta非常敏感。 -
The regeneration system of soybean cytoledon node and agrobacteriunr mediated transformation method is the first selection at present. in the second part of this experiment, the expression vector prok2 containing npt ii and ssnhx1 ( na + / h + antiporter ) gene from suaeda salsa was introduced into soybean cytoledon nodes by gene transformation mediated by agrobacterium tumefaciens, and kanamycin resistant transgenic p lants were obtained by screening in selective condition
本實驗第二部分通過農桿菌介導法將含npt -和鹽地堿蓬na ~ + h ~ +反向轉運蛋白基因（ ssnhx1 ）的表達載體prok2導入大豆子葉節中，經過含km的篩選培養基連續篩選，獲得了ssnhx1轉基因植株，篩選劑卡那黴素的適宜濃度是50mg . l ~ （ - 1 ） 。 -
Three chloroplast transformation vectors including pds16s - cat, ptn1269 - bar and psp72 - n5 - bar - n3 were constructed, using ! 6s rrna or chln gene sequence as a homologous segment and cat or bar as a selective marker gene, respectively. foreign genes were introduced to the cells of d. salina by microprojectile bombardment method and a pilot chloroplast tran
3 .杜氏鹽藻葉綠體165出na基因的克隆和轉化載體的構建根據杜氏鹽藻的近緣藻類的葉綠體基因組序列資料，克隆了杜氏鹽藻葉綠體16srrna基因部分序列1100bp ，並利用克隆的16srrna鄭州大學2003年博士學位論文 -
A gene library of psedomonas fluorescens g2 was constructed in the cosmid vector pla2917 using e. coli jm109 as the host strain. two recombinants, pgr3 and pgr7, which can confer glyphosate resistance ofe. coli jm109 were identified from the selective medium containing 10mm glyphosate
以粘粒pla2917為載體、大腸桿菌jm109為受體菌構建熒光假單胞菌g2的基因組文庫，在含有10mm草甘膦的固體選擇培養基上篩選出兩個耐受克隆pgr3和pgr7 ，插入片段分別為7kb和11kb 。 -
According to above consideration, experiments was carried out as below : first, targeted gene - human serum albumin ( hsa ) gene was obtained via pcr technology. secondly, the hsa gene was liganded with a plasmidprla22 whose two arms carry dna sequences necessary for crossing with the human a - lactalbumin yac to form an integration vector prla - hsa, then the integration vector plasmid was co - transformated into the right yac - bearing yeast with another plasmid plrh33 which carrys a selective gene
因此，本試驗首先擴增出整合在酵母基因組里的人血清白蛋白（ hsa ）基因作為目的基因，並將人血清白蛋白基因插入到一個含有人-乳白蛋白yac同源序列的重組型質粒載體，以構建整合型載體，再與另一個帶篩選基因的質粒共轉化入含人-乳白蛋白yac的酵母細胞體內。 -
No trace of any newly expressed protein band was noticed in supernant as well as in the cells by sds - page, except the verification of the substitution of beta - galactosidase gene ( the lose of galactosidase protein band ), which is a selective marker of the wild - type virus. elisa test results suggested the expression of egf in cells, but not in culture supernant. the quantitative calculation suggested the expressed egf was about 6 - 7 u g ( as egf standard ) per flask ( 2 > < 106 cells ) in the cellular extract
將重組病毒rbmbacph - egf以10moi感染bmn細胞， 72小時后收集培養細胞和上清；培養上清和經超聲波處理的細胞樣品elisa檢測發現胞內樣品中存在能與egf抗體免疫反應的產物，粗略估計表達量約6 7 g 2 10 ~ 6個細胞（相當于egf標準品） ；重組病毒rbmbacph - egf穿刺接種5齡家蠶幼蟲，每隔24h收集蠶血淋巴，經elisa檢測發現第4天表達量最高，根據egf標準曲線計算蠶血淋巴的表達量約32 g ml ； elisa定性實驗還發現正常蠶血也存在與egf抗體間交叉反應的物質。 -
To identify the transfected cells, we also constructed the gfp expressing plasmids which also attached the kozac sequence and nuclear localization signal to the gfp gene and the plasmids served as a selective marker for the transfected cells
為了區分被轉染的細胞，以附加kozac序列和核定位信號的gfp （ pcdna3 . 1 + kg ）作為被轉染的細胞的標記。 -
Beth showed 56 % identity to the opud of bacillus subtilis and belonged to bcct family. its putative promoter region was highly homologous to b - dependent promoter of b. subtilis. a 2. 6 kb fragment including the beth gene was subcloned into puc18 and transformed into the e. coli mkh13, colonies appeared on the plate of the selective m9 medium
將包括整個beth基因orf框及可能的啟動子核苷酸序列在內的2 . 6kb的片段克隆到puc18載體上，轉入到大腸桿菌甘氨酸甜菜堿缺失株mkh13中，使該菌株能夠在含甘氨酸甜菜堿的高鹽m9培養基上生長，而對照實驗不能生長。 -
We obtained our transgenic material, the rice suspension cells, by inducing embryonic rice callus. then we constructed the expression vector pca - ced9, and transferred ced - 9 gene into the rice callus and embryogenic suspension cells. the work of using hygromycin selective medium to obtain regenerated plants is still going
構建了用於水稻中表達的載體pca - ced9 ，通過農桿菌eha105轉化導入水稻愈傷組織和水稻懸浮細胞，經潮黴素（ hym ）篩選，以期望獲得抗性植株，目前該工作仍在進行中。 -
This system included method of solid medium plate enveloped with parafilm to form colony, conjugational gene transfer in a selective medium, screening the nonmagnetic mutants by magnet adsorption technique
此體系包括：以平板封膜培養技術獲得單菌落，在選擇性培養液中進行遺傳因子接合轉移，以液體培養和磁鐵吸附技術篩選突變子。 -
The genetic differentiation of among populations might be caused by human selective pressures and barriers to gene flow
群體間一定程度的遺傳分化可能是人為選擇壓力和基因流障礙引起的。 -
First, to enrich the selective markers for u32, two resistance genes emr and cat, one report gene amy were introduced into u32 to test their expression
首先對適用於u32的選擇性標記進行了篩選，結果表明來自puc19e的紅黴素抗性基因和來自pulam2的澱粉酶基因可以作為u32的選擇性標記。 -
Bar gene also is one of widely used selective marker gene. the cloning of bar gene is important to plant transgenic engineering, gene expression and studying physiology
而且bar基因也是迄今為止應用最為廣泛的一個抗除草劑選擇標記基因，克隆出bar基因對植物的遺傳轉化，基因表達和生理學研究都有重要的作用。 -
In the second part of the thesis, we described that a tobacco chloroplast expression vector, ptrvp1, containing the foot and mouth disease virus ( fmdv ) vp1 gene and the selective marker aada gene was constructed and transfered to the tobacci chloroplast genome by the biolistic method
論文第二部分主要敘述了煙草葉綠體表達載體ptrvp1的構建，並通過基因槍方法轉化煙草葉綠體基因組，獲得了3株具有壯觀黴素抗性的轉化再生植株。 -
2. an anther specific chimaeric male sterile gene expression box with a enhanced promoter ( ta29 ) driving coda gene was constructed and the expression box was inserted into binary vector p3301 that contains a l - phosphinothricin ( ppt ) - resistant selective marker gene and - glucuronidase ( gus ) reporter gene in t - dna region
以增強的ta29啟動子驅動克隆的coda基因，構建成花藥特異性嵌合基因表達盒；將此表達盒插入雙元載體p3301 ，構建成以ppt抗性基因為選擇標記，以gus為報告基因的植物表達載體。 -
To determine the site - specific integration of the foreign gene into the chloroplast genome and the level of homogeneity, the three transformation lines of the second selective generation were analysized through pcr ( pcr - southern blot ) analysis using the primers of fmdv vp1 gene and homologous fragment of tobacco chloroplast, respectively
經過兩輪抗生素篩選后，採用fmdvvp1基因的一對引物和煙草葉綠體同源片段的一對引物分別對抗性植株的葉綠體dna進行的pcr和pcr - southernblot分析。 -
The ced - 9 gene was transformed into leaves of tobacco by agrobacterium tumefaciens eha105. after twice selections of kanamycin cultures, 96 plants were regenerated from selective medium, and about 34 % of them turned out to be transgenic
然後以pbi121為對照組，通過根癌農桿菌eha105的介導，將ced - 9基因導入煙草葉片組織中，通過兩輪抗性篩選，得到抗卡那黴素（ km ）的再生植株。 -
First, a plant expression vector, pbbbt was constructed by inserting tsase into pbbb - etr to substitute the etr gene within the plant expression vector. then, it was introduced into the super - poison agrobacterium tumerfaciens eha105 through the " triparental mating " method. twenty regenerated tobacco plantlets were obtained with selection medium under the ppt selective pressure
首先將tsase基因取代植物表達載體pbbb - etr的etr基因構建植物表達載體pbbbt ，然後利用「三親交配」法導入超毒性農桿菌菌株eha105中，接著通過農桿菌介導法遺傳轉化煙草，經過連續的ppt抗性篩選，獲得20株抗性植株。 -
The gene is selectively inactivated or " knocked out " using a variety of genetic techniques ， and the effect of its selective deletion on that organism is determined
基因被選擇性的用多種遺傳技術滅活，在此生物體上選擇性去除的效果被確定。
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