sensitive (ml) 中文意思是什麼

In order to analyze the differences of the content of ergosterol in the imazalil - sensitive and imazalil - resistant isolates of p. digitatum, the technology of hplc was used. the results showed that the contents of ergosterol between imazalil - sensitive and imazalil - resistant isolates were not significantly defferent ( p = 0. 05, dmrt ) when they grew in the medium without imazalil. interestingly, when imazalil ( 0. 1 # g / ml ) was added to the medium, the content of the ergosterol in imazalil - resistant isolate ( pd07 ) was significantly higher than that in imazalil - sensitive isolate ( pd23 ) ( p = 0. 05, dmrt )

結果表明，在抗性菌株pd07不加抑霉唑的情況下， pd07和pd23中游離麥角甾醇的含量採用duncan ' s新復極差測驗在5水平沒有明顯差異；但當pd07中加入0 . 1 g ml抑霉唑時，三次重復測定結果顯示抗性菌株pd07中麥角甾醇的含量明顯地要比敏感菌株pd23中的含量高，而且兩者在5水平上差異顯著。

The results obtained were as follows : the mic of engineered peptide against staphylococcus aureus atcc 25923 ( penicillin sensitive strain ), atcc 29213 ( penicillinase - producing strain ) and atcc baa - 42 ( methicillin resistant strain ) were 1g / ml, 2g / ml, 4g / nil respectively. pi staining showed the staphylococcus aureus treated with the engineered peptide were dead

實驗結果表明:該工程多膚對三種atcc標準菌株atcc25923 （青霉素敏感菌株） 、 atcc29213 （產青霉素酶菌株） 、 atccbaa一42 （耐甲氧西林菌株）的mlc分別為1林留血l 、 2林留ml 、 4林創ml 。

The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides

進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。

Was added, the absorption of morin at 350nm decreased and a new absorption peak at 419nm appeared. adding of nucleic acids to the morin - al binary system leads to decrease of the absorption at 350nm and great increase at 419nm. there is an isochromatic point at 370nm. the increase of absorbance at 419nm is proportional to the amount of nucleic acids added within certain concentration range. based on this, a new sensitive nucleic acids analysis method is established. the linear ranges of ct dna, fs dna and y rna are 0. 7135. 4 0. 6425. 6 and 0. 9428. 4g ml, respectively

鋁離子的加入使桑色素350nm處的吸收峰下降，在419nm處出現桑色素-鋁絡合物的吸收峰。再往桑色素-鋁離子二元體系中加入核糖核酸或脫氧核糖核酸，則進一步引起桑色素350nm吸收峰的降低， 419nm處的吸收大大加強，同時在370nm處有一等色點。 419nm處吸光度的增加值與加入的核酸量在一定范圍內成正比，基於此建立了在較寬范圍內測定核酸的方法。

The nymphae and ten - day old female adult were more sensitive to the fungal spores than the thirty - day old female adult, and both then - nhs decreased significantly after injection with 4 + 107 spores / ml

若蟲和10日齡雌成蟲對綠僵菌孢子比較敏感，注射4 10 ~ 7個/ ml濃度的孢子后其血細胞數量均降低顯著，最低時分別為空白對照的4 . 6及8 . 5 。

The assay system of the biological activity of lymphotoxin was established using l929 cell as the sensitive target, lt international standard as the positive control and crystal violet staining method to detect viable cell after treated with lt. the best relationship between dosage and effect could be got if the cell seeding density in cell plate was 1. 6 0. 1 104 the dosage of amd was lug / ml, and the starting concentration of dilution in the plate of lt standard was 10 iu / ml with two fold dilution. the credibility of the established system was detected with rhtnfp developed by r & d

為確定經上述步驟純化后得到的目的蛋自lt 27的生物活性，本研究以l929細胞為靶細胞、淋巴毒素國際標準品為參照，採用結晶紫染色法檢測經淋巴毒素處理后存活的細胞，對淋巴毒素生物活性測定的細胞接種濃度、淋巴毒素標準品板上稀釋的起始濃度和梯度稀釋的倍數、放線菌素d的使用劑量等進行條件實驗后，建立了人淋巴毒素生物活性測定方法。

After the acet is vaporized, the active substance in water is gotten. and which is vaporized at low temperature. then the crude active substance is purified by column chromatography on sephadex g - 75. after a series of purifications again, we could get some white powder at last. though the active substance is diluted to50 g / ml, the activity is still checkeded - up through phyto phtnora casicileon. the purified active substance is insensitive to heat, resistant to chloroform ��� ethanol and the orhers. in addition, the active substance is sensitive to high ph ( 10 ~ 14 ), but it is not sensitive to low ph ( 1 ~ 5 ). furthermre, when the ph is made to low again, the activity of it ' s comes back

用蒸餾水對菌體稀釋；加入適量吸附樹脂在150rpm 、 28下振蕩吸附4h ， 80 %的丙酮解吸，過濾解吸液得到活性物質的澄清溶液，旋轉蒸發儀旋轉蒸發去處丙酮，經sephadexg - 75分子篩層析得單一活性峰，收集峰值部分樣品液經冷凍乾燥得到淡褐色粉末，該活性物質用丙酮充分洗滌、甲醇-乙醚重結晶獲得略帶微黃的白色粉末，該活性物質50 g / ml仍可對蘇雲金芽孢桿菌hd - 1產生明顯的抑制作用。

These vectors were tried to transform into the p. digitatum imazalil - sensitive isolate by the methods of peg - mediated or electroporation transformation. after that, the transformed protoplasts were regenerated on czapek medium containing hygromycin at 300 # g / ml. unfortunately, the ideal transformants could not be obtained

採用peg法和電擊法兩種方法進行指狀青黴原生質體的轉化，然後將得到的轉化產物轉移到查氏再生培養基中培養，遺憾的是沒有得到理想的轉化子。

Immuno - pcr for il - 6 was established by use of 1ng / l of the biotinylated dna as template. this immuno - pcr has a detection limit of 1fg / ml of pil - 6 that is 105 times lower than that of indirect elisa, and is the most sensitive method to detect il - 6 up to date

通過與elisa法比較，發現新建立的免疫pcr法靈敏度為1fg ml豬il - 6 ，是elisa法的10 ~ 5倍，是目前已報道的檢測豬il - 6最靈敏的方法。