sephadex gel 中文意思是什麼

sephadex gel 解釋
葡聚糖凝膠
  • sephadex : 降膽葡胺
  • gel : n. 凍膠，凝膠（體）；定型發膠。vi. （-ll-） 成凍膠，膠化。
  1. 6 - phosphogluconate dehydrogenase ( 6 - pgadase, ec 1. 1. 1. 44 ) was isolated by homogenate, ammunium sulfate fractionation, deae - sepharose chromatography, blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis, and some properties of the enzyme had been studied. a 113. 8 - fold purification was obtained with a 8. 2 % yield. the purified enzyme moved as a single electrophoretic band in page

    將枯草芽孢桿菌超聲波破壁后的粗提取物進行分段鹽析、 deae - sepharose陰離子交換柱層析， blue - sepharosecl - 6b特異結合柱層析和sephadexg - 200凝膠過濾等純化步驟，得到聚丙烯酰胺凝膠電泳為單一蛋白區帶，比活為1 . 46u mg的酶制劑。
  2. Under these conditions the fibrinolytic activity of the supernatant can reach 820 urokinase units per milliliter broth. ba - dfe was purified from the supernatant of b. amyloiquefaciens dc - 4 culture broth by ammonium sulfate precipitation, ion - exchange chromatography on cm - and deae - sepharose fast flow, hydrophobic interaction chromatography on phenyl sepharose 6 fast flow and gel filtration on sephadex g - 50. the purified enzyme displayed thermophilic, hydrophilic and strong fibrinolytic activity

    通過硫酸銨分級沉澱、 cm - sepharosefastflow和deae - sepharosefastflow離子交換層析、 phenylsepharose6fastflow疏水層析和sephadexg - 50凝膠過濾等方法，從解澱粉芽孢桿菌dc - 4的發酵液中分離純化出電泳純的ba - dfe 。
  3. An active metabolite was obtained by purification with precipitated by ethanol, sephadex g - 25 gel, deae - cellulose ion exchange resin and silica gel column chromatography

    經乙醇（ 95 ）沉澱、 sephadexg - 25凝膠層析、 deae -纖維素離子交換層析和硅膠層析純化，得到抑制黑麴黴生長的單一組分。
  4. In order to attain bioactive glycoprotein, glycoprotein of the leaves of camellia sinensis, was purified from coarse glycoproteins purified by sephadex g - 100 gel chromatography, by cona - sepharose 4b affinity chromatography

    為了純化天然糖蛋白，進行了茶樹葉糖蛋白的cona - sepharose4b親和層析，從sephadexg - 100凝膠過濾收集的粗糖蛋白中，分離茶樹葉糖蛋白。
  5. Many kinds of chromatograph including silica gel, d101 resin, sephadex l - 20 gel, rp - 8 and hplc are used in extracting and separating chemical constituents form these plants. certainly, they need to select the best eluate by tlc. some technique including ms ( ei - ms, fab - ms ), id and 2d nmr ( cosy, roesy, hmbc, hmqc ) been used in identifying these chemical structures

    通過各種硅膠柱層析，吸附樹脂， sephadex系列凝膠樹脂以及rp - 8類反向柱等層析材料以及高效液相色譜等技術，利用不同的洗脫體系對這兩種植物的化學成分進行分離得到純的單體化合物，然後利用ms （ ei - ms ， fab - ms ） 、一維nmr 、二維nmr （ cosy ， roesy ， hmbc ， hmqc ）等技術對這些化合物進行結構鑒定，利用ir和uv對這些化合物進行了表徵。
  6. An antifungal protein, named as b16, was purified from the supernatant of the fermentation broth of strain 041381 by 80 % saturation ammonium sulfate, desalt and gel filtration on sephadex g - 75, which with molecular weight at about 30 - 40 kd on the basis of sds - page

    菌株041381發酵上清液經70飽和度硫酸銨沉澱，透析脫鹽， sephadexg - 75凝膠過濾層柱，得到活性物質b16 。以sds - page膠為基礎進行電泳分析， b16的分子量為30 - 40kd 。
  7. The antimicrobial secretion could be divided into three sections, every section had antimicrobial activity. the first could be extracted by petroleum ether, the second could be extracted by ethyl alcohol, the third could dissove in water and could be separated by sephadex g - 50, g200 gel filtration chromatography and carbornmethyl cellulose - 1 anion - exchange chromatography and detected by a256, . the antimicrobial secretion had wide spectrum and had strong inhitory activity against germs and fungi, they could inhibit sixteen kinds of plant pathogenic germs, eight kinds of animal germs and eight kinds of plant pathogenic fungi

    粗提物經石油醚萃取可得第一個活性部分，剩餘部分經無水乙醇萃取可得第二個活性部分，剩餘物質再經凝膠sephadexg - 50後有兩個峰，第二峰有活性，再經凝膠sephadexg - 200後有三峰，第二峰有活性，將其經過陽離子交換柱cm - 1後有兩峰，此兩峰均有抑菌活性。
  8. One is a combination of ion exchange chromatography on q sepharose ff, hydrophobic interaction chromatography on phenyl hp, gel filtration chromatography on sephadex 200 and higher resolution ion exchange chromatography on mono q. the other is a combination of ion exchange chromatography on q sepharose ff, two affinity chromatography on con a sepharose 4b and benzamidine sepharose 6bcl sequentially

    上清液中重組蝗蛇毒類激血酶的純化是通過設計的不同分離純化方法組合進行的，並對每一種組合進行了活力與純度及口收率的評價，結果表明兩種組合方式都具有一定的實用性和可操作性。
  9. 3. the extracellular phb depolymerase was purified from 09 by using hydrophobic column chromatography and gel filtration technique in sephadex g - 100. the specific activity of the purified enzyme was increased by 37. 9 folds over crude extract, and the recovery yield was 8. 9 %

    以粗酶液為起始，經硫酸銨分級沉澱、 sephadexg - 100凝膠過濾后，分離純化了該酶，純化倍數約為37 . 9 ，酶活力回收率8 . 9 。
  10. The enzyme was purified from cell extract by ammonium sulfate fractionation ( 30 % - 80 % saturation ) and consecutive column chromatography using deae - cellulose and sephadex g - 100. the sod activity for purified enzyme could reach 4966 iu / mg proteins, and the molecular weight of the sod was about 20 kda which determined by sds - page electrophoresis. when the non - denaturing polyacrylamide gel stained with substrate for sod in the present of 0. 2mmol / l kcn or 0. 5 mmol / l h2o2, the active band was still showed at the same position, which indicated the sod could not be inhibited by the treatments with kcn or h2o2 and it was the mn sod

    通過sds一聚丙烯酸胺凝膠電泳可知該sod酶的分子量約為20kda .在非變性聚丙烯酞胺凝膠（ pagb ）電泳后，在凝膠son顯色反應液中加入0 . 2耐01 / lkcn或0 . 5inmol / lh202 ，凝膠sod顯色反應后在原來的sod酶部位仍然含有sod活性帶，這表明利用kcn和h20 :處理並不能抑制500的活性，該sod屬于mn一sod 。
  11. 4m phosphate buffer ; the second is that the crude enzyme runs on sephadex g100 - g50 column under the same experimental conditions ( only with sephadex g100, g50 ). at last polyacrylamide gel electrophoresis and sod assay tell us both of products are highly purified sod

    因而不管從第一次的純化來看，還是從文獻上報道的來看，本實驗的串聯柱層析是較為合理有效的一種生產方法，值得推廣。
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