sequence blasting 中文意思是什麼
sequence blasting
解釋
順序爆破-
By blasting the homologous sequences in genbank databases, the sequence of grass carp gh cdna from pituitary is 98 % homologous compared with the previously cloned gh cdna of grass carp. the cgh cdna fragment was inserted into pgex - 4t - l to construct the expression plasmid. the recombinant plasmid was digested by bamh i and ecor i to identify whether the cgh cdna fragment was inserted into the plasmid, the pgcgh was transformed into e. coli bl21 competent cells
將得到的序列在genbank和embl數據庫中進行了同源比較，結果顯示：本研究克隆到的草魚gh基因與genbank中登記的x60474草魚gh基因有12個堿基的差異，編碼的氨基酸有3個氨基酸殘基的差異，同源性為98 ，影響蛋白質高級結構的保守二硫鍵為2個。
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