sequence of total 中文意思是什麼

sequence of total 解釋
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  • sequence : n 1 繼續；接續；連續。2 順序；程序；次第；關系；關聯。3 後果；結果；接著發生的事；後事；後文。4 ...
  • of : OF ＝Old French 古法語。
  • total : adj 總計的（金額等）；全部的；完全的（失明等），絕對的（禁酒等）。 a total history 通史，全史。 a...
  1. The results indicate that : 1. the main physical and chemical characteristics vary regularly : with rising of the altitude, there is a transition from silt > sand > clay to sand > silt > clay in the mechanical composition ; the argic horizon emerges below the altitude of 1600 meters ; the content of organic matter is enrichment, the content of organic carbon of epipedon is higher than 20 g / kg, while the content of organic carbon increases with increasing of altitude, and in the altitude of 3500 - 3700meters, the soils under the meadow have the maximum content organic carbon ; the soils appear acid - slightly acid reaction, the ph decreases appreciably and acid strengthen with increasing of altitude ; the soils higher than the altitude of 2500 meters are base unsaturated, indicating the soil leaching is strong, the ph and bs are distinct plus correlated ; the contents of sio2, al2o3, and fe2o3 of the soil body and clay are all relatively stabilization ; in the soil body, the content of sio2 is much high and cao is very little, the total contents of sio2, a12o3 and fe2o3 occupy 92 % of the mineral parts, the sequence of mineral elements is : sio2 > al2o3 > fe2o3 > k2o > mgo > cao > tio2 > mno

    研究結果表明： 1太白山南坡土壤的主要理化性質隨海拔高度的上升呈有規律的變化：隨海拔高度上升，機械組成由粉粒砂粒粘粒逐漸過渡到砂粒粉粒粘粒，海拔1600m以下出現粘化層；土壤有機質豐富，表層有機碳含量一般在20g kg以上，有機碳含量隨海拔高度升高而相應增加，海拔3500 3700m的灌叢草甸植被下有機碳含量最高；土壤呈酸性或微酸性，並隨海拔上升， ph值略微降低，酸性增強，海拔2700m以上的土壤多呈鹽基不飽和狀態，表明土壤淋溶作用較強， ph值和鹽基飽和度呈極顯著正相關；土體與粘粒中的sio _ 2 、 al _ 2o _ 3 、 fe _ 2o _ 3含量相對比較穩定，土體中sio _ 2含量較高， cao含量較少， sio _ 2 、 al _ 2o _ 3和fe _ 2o _ 3含量之和約占土壤礦質部分的92 ，礦質元素含量的順序依次為： sio _ 2 al _ 2o _ 3 fe _ 2o _ 3 k _ 2o mgo cao tio _ 2 mno 。
  2. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  3. The protein accounting for the total was approximately 11. 80 %. the peptide was synthesized according to the sequence of grb - ast7. since ast7 is a hapten, it was conjugated to the carrier proteins bovine serum albumin ( bsa ) using l - ethyl - 3 - ( dimethyl - aminopropyl ) carbodiimide ( edc )

    根據ast _ 7氨基酸序列合成小肽，用edc做偶聯劑把合成的半抗原小肽與載體bsa偶聯成完全抗原，免疫家兔三次后，采血收集抗血清，用elisa測定效價為1 400 。
  4. A pair of primers were chemically synthesized based on the cdna sequence of hog cholera virus strain brescia and used to amplify the cdna fragments of envelope glycoprotein e2 gene of hclv which coding the major protective antigen by reverse transcription - polymerase chain reaction ( rt - pcr ) from total intact rna extracted from infected calf testicle cell culture by hclv

    以豬瘟兔化弱毒犢牛睪丸細胞毒（ hclv ）中提取的細胞總rna為模板，以rt - pcr技術，擴增出了完整e2基因的cdna片段。經電泳、酶切分析，證實了所擴增片段為e2基因特異性片段。
  5. The sequence of lead content in each hyperaccumulator was bidens maximowiciziana > amaranthus tricolor > sophora japonica > xanthium sibiricum > schizonepeta fenuifolio > vetiveria zizanioides in this study. on one hand, bidens maximowiciziana and amaranthus tricolor could transport the iiiost of the lead from roots to the above - ground parts, and the total heavy metal translocated ( tmt ) of bidens maximowiciziana and amaranthus tricolor rose to the 11. 29mg and 9. 17mg in 100 plants

    結果表明，羽葉鬼針草和綠葉莧菜能把吸收的pb較多地運輸到地上部， 100棵羽葉鬼針草和綠葉莧菜地上部的遷移總量高達11 . 29mg和9 . 179mg ；羽葉鬼針草根系對pb的耐性最強，當pb處理濃度為loomg
  6. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  7. The moderate insect includes 227 species, which hold the 61. 52 % of the whole community. the total quantity of pest in the three experimentation sections is 4935, and the sequence of quantity to the three sections is blank section ( 2213 ) > harmless section ( 1551 ) > general section ( 1171 ). the key pests are olethreutes leucaspis meyrick, conopomorpha sinemis bradley, xyleborm fornicatus eichhoff, dasineura sp

    三個試驗區害蟲調查總數量為4935頭，各區數量大小依次為：空白區（ 2213頭）無公害區（ 1551頭）常規區（ 1171頭） ，關鍵害蟲為三角新小卷葉蛾，荔枝蛀蒂蟲，茶材小蠹，荔枝葉癭蚊，荔枝癭蟎，樟翠尺蛾，荔枝蝽。
  8. The sequence of two patterns presence is changed alternately, that is, one pattern will appear at the second pulse of total pattern in this half cycle if it appears at first pulse in last half cycle. the stability of square pattern was studied by considering the interaction among the wall charges. the discharge moments of individual filament alternate from long one to short in the square pattern, which can been explained by using the breakdown and quench model through considering the wall discharge accumulated on the dielectric layers

    實驗研究了正方網格斑圖與混合氣體的比例及外加電壓的關系，給出了班圖類型隨上述條件變化的相圖；實驗採用光學方法對正方網格斑圖進行了時空動力學測量，發現正方網格斑圖是由兩套正方網格斑圖相互嵌套而成，其中一套的微放電絲位於另一套正方形單元的中心，這兩套微放電絲交替進行放電；考慮到壁電荷之間的相互作用，研究了正方網格斑圖的穩定性；實驗發現正方網格斑圖的微放電絲放電時間間隔是長短交替變化的，考慮到電介質表面積累的壁電荷的作用，使用擊穿?熄滅方程很好的解釋了該現象。
  9. The total rna was purified from the germ in the liquid by the guanidine isothiocyantehod method, then the total rna digested by dnase that had not rnase was used for rt - pcr. i change the magnesium ion dencity in the pcr system in order to optimize the pcr condition. at the end i selected the magnesium ion density as 1. 25 mm. the production of rt - pcr was inserted directionally into pgem ? z ( ampr ). the pgem ? z ( ampr ) was used to transform e coli jm109. i got a positive clone through culling and identificatin. the dna sequence inserted into pgem ? z ( ampr ) was sequenced and blasted with the cdna sequence of the # - mannanase mature peptide that got from genbank

    分取誘導培養液中的菌體，用異硫氰酸胍法提取總rna ，總rna再經無rna酶的dna酶處理後用于rt ? pcr 。在pcr擴增目的基因時，通過優選擴增體系，使鎂離子濃度為1 . 25mm時rt ? pcr可順利地獲得目的基因，並能定向克隆到載體pgem ? 3z （ amp ~ r ）中。用克隆載體轉化宿主大腸桿菌jm109 ，通過篩選獲取陽性克隆子，對陽性克隆子進行酶切與pcr鑒定，並對載體中插入的目的基因進行測序。
  10. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  11. According to the described sequence of chicken interleukin - 1 ( chil - 1 ) and by using the extracted total rna of chicken peripheral blood mononuclear cell ( pmbc ), a pair of primers were designed to reverse transcriptionally amplify and to compare the expressional degrees of chil - 1 mrna by stimulating chicken pmbc for 4, 8, 12, 24 hour with lipopolysaccharide ( lps ) and for 24 hour with lps and actidione respectively. the results showed that stimulating chicken pmbc with lps and actidione to produce chil - 1 was a better way of artifical stimulation

    根據國外報道的雞il ? 1序列，設計引物，以提取的雞外周血單核細胞總rna為模板， rt ? pcr擴增並比較了經lps單獨刺激雞外周血單核細胞4h 、 8h 、 12h 、 24h和lps與放線菌酮聯合刺激24h時il - 1 mrna表達水平，發現lps與放線菌酮聯合刺激雞外周血單核細胞是一種較佳的實驗方案。
  12. Sequence of total

    電文頁次
  13. The complete genome sequence of the radiation - resistant bacterium deinococcus radiodurans rl is composed of two chromosomes ( 2, 648, 638 and 412, 348 base pairs ), a megaplasmid ( 177, 466 base pairs ), and a small plasmid ( 45, 704 base pairs ), yielding a total genome of 3, 284, 156 base pairs

    一號染色體由2 ， 648 ， 638個堿基對組成，二號染色體包含412 ， 348個堿基對，而大質粒和小質粒分別由177 ， 466和45 ， 704個堿基對組成。它們共同組成了全基因組3 ， 284 ， 156個堿基對。
  14. The gene encoding the mature peptide was cloned from the total rna of h rhossiliensis owvt1 by rtpcr. sequence analysis of the gene was described in this papel the amino acid sequence, as derived from the nucleotide sequence of a cdna clone, had high homology with other subtilisin - like serine protease of nematogenous fimgi

    與其他絲氨酸蛋白酶基因序列比較表明，與其他線蟲卵寄生性真菌如paecilomyceslilacinus 、 verticilliumchlamydosporium及m . anisopliaevar . anisopliae同源性較高，而與捕食線蟲真菌arthrobotrysoligospora同源性較低（ 45 ） 。
  15. For instance, embranchment working procedure, embranchment point working procedure, working procedure total, working procedure and correspond process time. moreover, this thesis worked out priority area chart. it was based on successively sequence of process manufacture and principle to lessen number of one man working more machine to the best of our abilities

    從預處理過的工序分析表可以找出分支工序、分支點工序、工序總數、各工序及相應工序的加工時間等數據信息，另外根據工序加工的先後順序和盡量減少一人多機的原則，做出了優先權區域圖。
  16. Ii ) two fragments ( about 240bp and 130bp ) were amplified from human keratinocytes total rna by rt - pcr. the recombinant pm - hpabl and pm - hpabs plasmids were constructed by inserting 240bp and 130bp pcr products into pmd 18 - t vector, respectively. the recombinants were identified by restriction enzyme analysis and dna sequencing, iii ) two orfs ( > 100bp ) were found in the insert sequence of pm - hpabl

    應用smartpcr試劑盒和簡並引物從人皮膚角質形成細胞cdna中擴增到長分別為24obp和13obp左右的2種片段，將它們插入pmd18一t載體，用酶切法初步篩選陽性重組子pm . hrabl和pm一hrabs ，對陽性重組子進一步作測序鑒定。
  17. In this research, total rna was extracted from fetal liver by the modified single - step method of acid guanidinium thiocyanate - phenol - chloroform ( agpc ). based on reported sequence of hsa cdna, specific primers were designed to amplify its encoding region by rt - pcr. at the same time, kozak sequence was added to the translation site, to strengthen initiation of translation

    本研究通過改進的異硫氰酸胍-酸性酚-氯仿一步法從人工流產胎兒肝臟中提取總rna ，然後根據報道的序列設計特異性引物，同時在上游引物中引入了kozak序列，經rt - pcr克隆到hsacdna編碼區序列，長度為1758bp 。
  18. According to the derived spore - pollen percent diagram and spore - pollen concentration diagram, the sequence of vegetation change recorded in the malan loess of xishan mountain, beijing area is as follows : zone p1 : in this zone the herbal pollens were overwhelming and the total pollen concentration was relatively high, so the vegetation during this period was arid grassland

    P3帶，該帶總體上花粉濃度較小，花粉濃度出現了多次波動，表明該時期氣候不穩定，植被在荒漠草原和乾草原之間變化。該帶可以分成下列5個亞帶： p3 ） ， p3 2p34pm和p3 5 。
  19. " we received total co - operation from hkcec s management and staff, " he said, " and so could stage a sequence of very different events within hours with no logistical or crowd problems, plus the fullest possible facilities and opportunities for the world s media, including the 9, 000 sq m press and broadcast centre.

    他續說：幸而得到會展中心管理層與員工的衷誠合作，才得以在短短數小時內進行一連串不同類型的活動，而絲毫沒有造成任何操作上或控制人群的問題。
  20. After rt - pcr of total rna isolated from the ovaries of lagurus lagurus, the lzp3 cdna was cloned into pucm - t and further identified with sequence analysis

    將擴增產物連接到pmd18 - t載體上進行測序，序列分析表明已克隆出lzp3全長cdna 。
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