sequenced chain 中文意思是什麼
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G gene of rabies virus m, the of two main regions ( about 1000nt ), ranging from 3161nt to 4162nt and ranging from 4012nt to 4863nt of glycoprotein gene of rabies virus strain m, isolated from mouse in he nan, china were amplified by reverse transcriptase - polynerase chain reaction ( rt - pcr ) in order to complete glycoprotein gene of strain m. these regions were sequenced by the produce of pcr directly. comparison and analysis of nucleotide sequence and amino acid sequence deduced with that of other strains published was performed by computer with dnasisv 2. 5demo software
本研究對我國河南某地野鼠體內分離的狂犬病野毒株mrv基因的3161位? 4162位（ 1001個堿基）和4012位? 4863位（ 851個堿基）片段進行了反轉錄pcr擴增和序列測定，得到mrv的糖蛋白基因全序列，用dnasisv2 . 5demo分析軟體，與已發表的代表性毒株g基因全序列進行核苷酸和氨基酸序列的比較分析，結果表明在同一基因型中， mrv和國際標準攻毒株cvs的同源性最高（ 96 . 5 ） ，和中國減毒株ctn的同源性最低（ 79 . 8 ） 。 -
The percentage of polymorphic sites, degree of genetic polymorphism and genetic distance were compared and the phylogenetic tree was constructed by neighbor - joining method. the partial mitochondrial 16s rrna gene was amplified by polymerase chain reaction ( pcr ) and the pcr products were directly sequenced after purified. these sequences, together with the homologous sequences of another trichiuridae species lepidopus caudatus obtained from genbank were used to analyze nucleotide difference and to establish a upgma phylogenetic tree by means of biological informatics
汝us價ay1830 ）各12個個體進行rapd分析，對比多態位點比例、遺傳多態度以及遺傳距離，並構建neighbor - join噸系統樹;通過pcr擴增出線粒體165rrna基因，純化后直接測序，利用生物信息學方法進行序列分析和核昔酸變異比較，結合ge紅bar止中大西洋叉尾帶魚（ lepid （護腳caud玫tuseuphrasen1788 ）同源序列構建u甲cm叭系統樹。 -
Vsc - ryerson china commits to the highest degree of quality services in every sector of the steel supply chain from steel mills to end users, such as jit, and sequenced delivery, tailored to customer needs, it implements supplier consolidation with proven cost saving programs to provide the customer with efficient quality services at competitive costs
萬順昌瑞爾盛中國有限公司致力於提供從鋼廠到最終用戶的所有供應鏈環節的高質量服務，諸如：準時生產、逐一交貨、按客戶需求，用降低成本的供應商集成配送系統去提供客戶以具有竟爭力的質量服務。 -
The comparison of meq gene sequences of different pathotypes of marek ' s disease virus meq genes of different pathotypes of marek ' s disease virus ( mdv ) were amplified, in the whole opening reading frame ( orf ) by polymerase chain reaction ( pcr ) technique, sequenced and compared with the published sequence of ga strain ( representing vmdv ). cvi988 / rispens and 814, commercial vaccine strains popularly used worldwide and in china respectively ; 648a, representing very virulent plus ( vv + mdv ) and 6 field isolates, originated from guangxi commercial chickens with visceral lymphomas and vaccine breaks, representing high pathogenic ( hpmdv ) and two of them ( g2 and n ) were proved to be vvmdv, were used
本研究通過三個方面進行了研究，取得了如下主要實驗結果： 1mdv不同致病型（ pathotypes ）毒株meq基因序列的比較研究應用pcr技術擴增了不同致病型mdvs ，即國際通用型疫苗毒cv1988 rispens株和中國特有的疫苗毒814株、特超強毒648a株（ vv + mdv ）以及6個廣西分離到的野毒株共9個毒株的meq基因，進行核苷酸序列的測定並與標準強毒ga株（ vmdv ）進行核苷酸和氨基酸序列的比較。 -
The study on the function and mechanism of phrip1 is important for clarifying how the cell plate and cell wall form in plants. in this study, full length of phrip1 is amplified by pcr and ligated into pks plasmid, then the bait plasmid, peg202 - phrip1, is constructed. the inseret gene are sure to be translated into the right fusion protein through its sequence. in the yeast two - hybrid system, the bait plasmid ( peg202 - phrip1 ) and a reporter plasmid ( psh18 - 34 ) are introduced into the yeast ( egy48 ) by co - transformation. then cdna library ( which is in pjg4 - 5 ) is screened and two genes are obtained. the two insert gene fragments are sequenced. one of them is plastocyanin, the other is putative photosystem i reaction center subunit ii precursor, both of them are the necessary components of photosynthetic chain
成膜素相關蛋白1 （ phrip1 ）是一個含608個氨基酸的蛋白質，它對于植物胞質分裂中細胞板的形成起到了十分重要的作用。研究phrip1的功能和機制，對在分子水平上闡明植物細胞板以及細胞壁形成的機理具有重大的生物學意義。在本實驗中，根據phrip1的序列設計引物對其進行pcr擴增，得到該基因后將其連接到了pks質粒上，並進一步構建成了誘餌質粒peg202 - phrip1 。 -
The proviral genome cdna clone of snv strain was digested, and the fragments was cloned into the puc18 vector, sequenced, finally we got the sequence of snv genome. the two pairs of primers were designed and synthesized according to the snv strain env sequence. the env genes of the rev isolates isolated from china were amplified by polymerase chain reaction ( pcr )
根據網狀內皮增生癥病毒snv株env基因的序列，設計並且合成了兩對引物，利用該引物，以中國地方分離株sd9901 、 ha9901前病毒基因組cdna為模板，通過pcr技術，成功的從國內分離得兩株rev毒株中擴增出env基因，並將之克隆進pucm - t載體中測序。 -
These results showed that the three isolates were infectious bursal disease virus. the full - length cdna of the genomic segment a of two viruses, one virulent field strain ibdv zj2000 and one attenuated strains ibdv jd1, were amplified in a single step procedure by long - accurate reverse - transcription polymerase chain reaction ( la - pcr ), cloned into pgem - t easy vector, and sequenced
分別以傳染性法氏囊病病毒zj2000株（野毒株）和jd1株（弱毒株）基因組dsrna為模板，採用long - accuratert - pcr （ la - pcr ）一步法擴增並克隆了兩株病毒的基因組a節段全長cdna 。 -
To investigate the distribution of gene mutations in lpl and to study the relationship between lpl mutations and plasma lipid, the lpl gene ( exon5 ~ 9 including intron - exon boundaries ) was examined by pcr - sscp analysis, and the amplified products showing abnormal pattern on sscp were sequenced using dideoxy - mediated chain - termination
在外顯子5中，檢出一雜合子突變，經dna序列測定證實為pro （ 207 ） leu突變雜合子，該患者為高甘油三酯血癥病人。
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