sequenced frame 中文意思是什麼
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The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size. computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70. 3 % that would encode a protein of 552 amino acids ( aa ). the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp
對phz1754進行外切核酸酶（ exonuclease ， exo ）順序缺失，獲得單向長度漸減重疊的系列突變體，核苷酸序列測定顯示出該ecor - sal片段的精確大小為2299bps ， frameplot程序分析揭示出該區域一個完整的開放閱讀框（ orf ）的存在，其大小為1656bps ， g + c含量為70 . 3 ，編碼552個氨基酸，利用blastsearch程序將orf的核苷酸序列及推導的氨基酸序列與因特網上基因及蛋白質數據庫進行綜合比較，發現無論在核苷酸水平還是在蛋白水平上，該orf均與膽固醇氧化酶表現出同源性，而且與鏈黴菌膽固醇氧化酶同源性最高，說明該orf編碼膽固醇氧化酶基因。 -
Nucleic acid sequences of azoreduclase were searched and blasted in genbank. a pair of primers based on the conserved regions were designed. a specific fragment was amplified by pcr from the plasmid of rhodopsedomonas palustris and sequenced. the sequence contained a complete 471bp orf ( open reading frame )
脫色實驗證明沼澤紅假單胞菌（ rhodopsedomonaspalustris ）對偶氮染料有較強的降解能力，我們通過genbank搜索，對所獲得的所有偶氮還原酶基因在ncbi進行比對並設計引物，從沼澤紅假單胞菌質粒中擴增獲得了一條含471bp完整開放閱讀框架的序列。 -
The recombinant plasmid was identit1ed with restriction endonuclease, pcr, then sequenced. the resuit of sequence analysis showed that the vp3 gene is 1605bp and inc1uds a complete open reading frame encoding a protein of 534 amino acids. the hl isolate shares 98. 5 % and 98. 3 % identity with b isolate at nucieotide and amino acid levels respectively
結果表明：鵝細小病毒h1分離株vp3基因全長1605bp ，編碼534個氨基酸，只有一個完整的開放閱讀框架，與國外已發表的鵝細小病毒b株核苷酸序列同源性為98 . 5 ，氨基酸序列同源性為98 . 3 ，表明這二個毒株親緣關系相近。 -
The comparison of meq gene sequences of different pathotypes of marek ' s disease virus meq genes of different pathotypes of marek ' s disease virus ( mdv ) were amplified, in the whole opening reading frame ( orf ) by polymerase chain reaction ( pcr ) technique, sequenced and compared with the published sequence of ga strain ( representing vmdv ). cvi988 / rispens and 814, commercial vaccine strains popularly used worldwide and in china respectively ; 648a, representing very virulent plus ( vv + mdv ) and 6 field isolates, originated from guangxi commercial chickens with visceral lymphomas and vaccine breaks, representing high pathogenic ( hpmdv ) and two of them ( g2 and n ) were proved to be vvmdv, were used
本研究通過三個方面進行了研究，取得了如下主要實驗結果： 1mdv不同致病型（ pathotypes ）毒株meq基因序列的比較研究應用pcr技術擴增了不同致病型mdvs ，即國際通用型疫苗毒cv1988 rispens株和中國特有的疫苗毒814株、特超強毒648a株（ vv + mdv ）以及6個廣西分離到的野毒株共9個毒株的meq基因，進行核苷酸序列的測定並與標準強毒ga株（ vmdv ）進行核苷酸和氨基酸序列的比較。 -
Sequence analysis data indicated that 99 % homology was indentified between the published bovine follistatin cdna gene and my two - directional sequenced products. the sequence data also shows that the open reading frame of the pet - 30a - fs2 is correct
將獲得的follistatincdna基因克隆入原核表達載體pet - 30a中，組裝成了原核表達載體pet - 30a - fs2 ，經正反測序表明其閱讀框正確。 -
The positive cloned was sequenced by sanger " s dideoxy sequencing method. the results demonstrated the size of hn gene was 1930bp in length and included an open reading frame encoding a protein of 578 amino acid residues
經核苷酸測序，該基因全長1930bp ，含一個1734bp的開放閱讀框，編碼578個氨基酸，與國外報導的hn基因相比，核苷酸同源性為95 -
The specific recombinant plasmid was identified by molecular weight, pcr and restriction endonuclease analysis. the results indicated that the resuctant construct contained the gene of interest f. the positive cloned was sequenced by sanger ' s dideoxy sequencing method. the results demonstrated that the f gene included an open reading frame which was 1662bp in length and encoded a protein of 553 amino acid
經核苷酸測序，該基因含一個1662bp的開放閱讀框，編碼553個氨基酸，與國外報導的新城疫各毒株f基因相比，核苷酸序列同源性為88 . 3 96 . 4 ，氨基酸同源性為92 . 1 96 . 4 。
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