serine protease 中文意思是什麼

serine protease 解釋
絲氨酸蛋白酶
  1. The he was strongly inhibited by sbti and apmsf, and very sensitive to pmsf, lbti, and tlck, while not sensitive to chymostatin, bestatin, leupeptin, tpck, pepstatin, nem and iam. all these results imply that brine shrimp he was most probably a trypsin - type serine protease. the he could be strongly inhibited by edta in a dose - dependent manner, and 50 mmol / l edta exhibited more than 56. 5 % inhibition

    對孵化酶純化樣品進行生化性質和酶性質分析發現，鹵蟲孵化酶的最適反應溫度約為40 ，最適ph為8 . 5左右；該孵化酶對p - apmsf 、 sbti極為敏感，對pmsf 、 lbti和tlck也非常敏感，但對chymostatin 、 leupeptin 、 pepstatin 、 bestatin 、 tpck 、 nem和iam不敏感，表明該酶極可能是一種屬于胰蛋白酶類型的絲氨酸蛋白酶。
  2. After careful studying their relative importance to immune response and the possibility of the match, seventeen sequences of interest were selected for further experiment, including estss analogous to 11. 5kd antibacterial peptide, lysozyme, serine protease and its inhibitor, lectin, antifreeze protein, et al. primers designed according to the sequences were used to amplify the corresponding estss from both blood and cephalothorax cdna library

    在仔細分析了它們在免疫系統中的重要性和在對蝦中出現的可能性之後，從中選出了17條可能編碼抗菌肽，溶菌酶，凝集素、絲氨酸蛋白酶及其抑制劑，抗凍蛋白等蛋白質的序列，以此為依據設計引物，在中國對蝦的血液和頭胸部cdna文庫中擴增相應的序列。
  3. The masp ( mannose - binding lectin - associated serine protease ) gene has been cloned by the method of degenerative pcr and the fragment of the pcr product is 630 bps in length

    本文還利用pcr方法從青島文昌魚基因組dna中克隆masp （甘露聚糖結合凝集素相關絲氨酸蛋白酶）基因片段。
  4. Based on the extensive studies of subtilisin - like protease ( prl ) of metarhizium anisopliae, extracellullar serine protease is suggested to be a key enzyme involved in the fimgal penetration to invertebrates. the investigation of serine protease in the nematode infected by owvtl may help to understand the mechanism of nematophagous fimgi as biological control agents. a 3l kda serine protease was isolated and purified from the liquid culture of h rhossiliensis owvtl challenged with nematode panagrellus redivivus

    本研究利用線蟲誘導下owvt - 1菌株液體發酵，通過粗分級分離、離子交換層析和凝膠過濾層析分離提純了一個分子量為31kda的絲氨酸蛋白酶，生物學測定表明其對大豆胞囊線蟲二齡幼蟲具有致死作用，同時測定了該酶理化特性，酶活力在75附近酶活力最高，隨著ph的增加酶的穩定性升高，與膽堿酯酶具有相似的ph曲線，對特異性底物aape （ suc - ala - ala - pro - glu - pna ）具有作用， ssi和ci - 2抑制該酶的活性。
  5. Sequence alignment shows that the fragment of pcr product showed some identities to the masp gene of xenopus laevis, branchiostoma belcheri, the trypsin gene in litopenaeus vannamei and the serine protease gene in aurelia aurita

    Pcr反應產物基因片段大小為630bp ，序列比較結果表明， pcr克隆產物與爪蟾、文昌魚的masp基因，蝦、水母等的胰蛋白酶基因和絲氨酸蛋白酶基因都有一定的同源性。
  6. A web - based blast is also constructed to facilitate the process. and the contigs and ests sequences are formatted as subject database. by similarity search, about 442 estss were found possibly encoding immune related proteins, such as lysozyme, lectin, serine protease, serpin, thioredoxin, sod and so on

    為了方便相似性比對，在由四臺pc機并行組成的機群（ pc - cluster ）上將blast程序和interproscan程序本地化，並且將contig和ests序列文件做成blast程序中的序列庫文件以供搜索。
  7. Huwentoxin xi ( hwtx ~ xi ), a serine protease ! inhibitor, consists of 55 amino acid residues with three disulfide bridges. the toxin was isolated from the venom of the chinese spider ornithochoctonus huwena by ion - exchange chromatogram - phy and reverse phase high performance liquid chromatography

    本文報道從虎紋捕鳥蛛（ ornithochoctonushuwena ）粗毒中，應用陽離子交換和反相高效液相色譜的方法分離到一種胰蛋白酶抑制劑，命名為huwentoxin - （ hwtx - ） 。
  8. The full orp encodes a 487 - amino acid protein with a calculated molecule weight of 53. 484 kda and an isoelectric point of 6. 75. at the primary sequence level, it shared high homology with clr, so was named clr - like serine protease, clsp. the clsp sequence has been submitted to genbank / embl ( genbank accession number af178985 )

    該蛋白在核酸和氨基酸水平上與人補體組分cl :表現高度同源，故將其命名為補體cl :樣絲氨酸蛋白酶（旦lr一like旦erineprotease ， clsp ） ，該基因已經在ge心ank登錄（登錄號為af17s985 ） 。
  9. The clsp protein shared roughly 25 - 40 % identity and 40 - 55 % similarity to complement subcomponent clr, cls, mannose - associated serine protease - 1 ( masp - 1 ), masp - 2, and masp - 3

    通過在genba川k / embl數據庫中對clsp序列進行同源性搜索和分析，我們發現其核酸和氨基酸序列和人clr 、 cis 、 maspz 、 maspz 、 masp3等有高度同源性。
  10. The result of sequencing shows that this fragment contains an open reading frame of 1106 nucleotides and encodes 369 amino acids residues, which is part of a alkaline serine protease, with 98. 3 % homogeneity to a reported gene from bacillus pumilus

    序列分析顯示，該片段是一個全長1106bp的orf ，可編碼369個氨基酸殘基的多肽，與短小芽孢桿菌堿性蛋白酶基因編碼區高度同源。
  11. Usually, clr is binding to c1inh ( cl inhibitor ). when immune complex ( ic ) binds to clq, c1inh is detached form clr, and then the serine protease is activated, and then the complement classical pathway is activated

    在一般情況下， c1r與c1inh （ c1inhibitor ）結合著，而一旦有免疫復合物（ ic ）結合到c1q上時，浙江大學碩士學位論文cllnh的抑制作用即行移除， clr的構象發生改變，具有酶活性，從而啟動補體激活經典途徑。
  12. Comparison of the entire derived peptide sequence with other serine protease revealed significant homology, especially with a reported gene from another b. pumilus strain tyo - 67 ( 99 % homology )

    將克隆到的堿性蛋白酶基因（命名為ap ）編碼的成熟蛋白n一端序列與純化的脫毛蛋白酶n一端序列進行比較，二者完全相同。
  13. For trypsin - like serine protease domain, the presence of his ( h ) - asp ( d ) - ser ( s ) representing a serine protease catalytic triad were at position of 283, 339, 436, respectively

    Clsp在正常人外周血單核細胞來源的dc中有較強的表達，在分別經anti一cd40 、 tnf一a 、 lps刺激后的dc中，其表達水平均有所上升。
  14. Bioassay showed that the second - stage juvenile ( j2 ) could be killed by the raw extract of serine protease. serine protease was characterized after purification with ion exchange chromatography and gel filtration chromatography. the t profile, t stability profile, ph profile, substrate specificity and inhibitor were tested

    該酶分子n -端的氨基酸序列為avidtgveashpef ，通過n -端氨基酸序列設計簡並引物，提取被毛孢owvt - 1的總rna ， rt - pcr克隆了該酶的成熟肽編碼基因（ 1000bp ） ，序列分析表明，絲氨酸含量高達12以上。
  15. The result of blastx shows that one orf is extracellular serine protease precursor gene ; 3 orfs function as regulatory genes ; 5 orfs are involved in secretion pathway ; 2 orfs are related to production of lps ; and the annotation functions of the other 3 orfs are not clearly related to extracellular protease prouduction. marker exchange method was used to study the relationship between the production of protease and the 3 orfs. a deletion mutant of xcc _ 4463 was constructed successfully

    Orf注釋表明，共中一個基因為胞外蛋白酶結構基因， 3個基因與合成調控有關， 5個與轉運分泌有關， 2個與lps合成有關，其餘3個orf的注釋功能與胞外蛋白酶的關系未見報道，為研究這3個orf與胞外蛋白酶產生的關系，採用同源雙交換orf缺失法進行了進一步驗證，成功地構建了xcc _ 4463缺失突變體，所得缺失突變體經檢測胞外蛋白酶減少，在寄主上致病性降低。
  16. Hydrophobicity analysis predicted a 36 - residue hydrophobic signal peptide for secretion in the n - terminus, and no transmembrane region was present, suggesting it might be a type of secretory protein. some generic and atipical n - glycosylation sites were present in clsp, suggesting that the protein might represent a glycoprotein. the clsp protein contained one cub ( clr / cls, an embryonic sea urchin protein uegf, and bone morphogenetic protein i ) domain from 39th to 164th ammo acids, which is known to be involved in protein - protein or protein - substrate interaction, and a trypsin - like serine protease domain positioned from 244th to 483rd amino acids

    Clsp分子具有補體樣絲氨酸蛋白酶的多種結構特徵，包括36個氨基酸組成的疏水信號膚，一個cub （ clr / cls ， anembryonicseaurehinproteinuegf ， andbonemorphogeneticproteinl ）結構域和一個胰酶樣絲氨酸蛋白酶結構域（ t汀psin一likeserineproteasedomain ）和幾個保守的糖基化位點等，沒有發現有跨膜區的存在。
  17. The gene encoding the mature peptide was cloned from the total rna of h rhossiliensis owvt1 by rtpcr. sequence analysis of the gene was described in this papel the amino acid sequence, as derived from the nucleotide sequence of a cdna clone, had high homology with other subtilisin - like serine protease of nematogenous fimgi

    與其他絲氨酸蛋白酶基因序列比較表明，與其他線蟲卵寄生性真菌如paecilomyceslilacinus 、 verticilliumchlamydosporium及m . anisopliaevar . anisopliae同源性較高，而與捕食線蟲真菌arthrobotrysoligospora同源性較低（ 45 ） 。
  18. The homologous analysis showed that the n - terminal sequence of dhap is identical to that of serine protease from another b. pumilus strain tyo - 67 and has high homology with those from other bacillus species. based on the homologous analysis, three pairs of primers were designed and synthesized

    該序列與來自其它芽抱桿菌屬菌株的絲氨酸蛋白酶的n一末端氨基酸序列有較高的同源性，並和另一株短小芽抱桿菌的絲氨酸蛋白酶n一末端氨基酸序列完全一致。
  19. The clsp gene is located approximately 3 kb and 90 kb centromeric to the clr and cls gene, respectively, and their genes form a short cluster in a region of about 115kb in 12pl3. the cluster gene arrangement and the sequence similarities of the cub and trypsin - like serine protease domain of clsp with other complement component 1 subcomponents supported a common evolutionary history by consecutive gene dup

    同樣利用pcr檢測了clspmrna在人正常組織中的分佈，結果發現，人clspmrna廣泛表達於人正常組織中，如在胎盤、肝臟、腎臟、胰腺中表達量較高，在肺、脾、前列腺、卵巢、結腸、外周血組織有中度表達，而在心臟、骨骼肌、胸腺、翠
  20. The cub domain and serine protease domain 01 clsp closely resembled the corresponding domains of clr ( 48 % and 58 % identity, respectively )

    Clsp的cub結構域和絲氨酸蛋白酶結構域與clr相應的結構域之間的相似性（ simllarity ）分別為為48 %和58 % 。
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