serum-free medium 中文意思是什麼
serum-free medium
解釋
無血清培養液,無血清培養基-
Methods : cell culture in serum - free medium, indirect immunofluorescence cytochemistry were used
方法：採用無血清細胞培養技術，間接免疫細胞化學染色法。 -
Abstract : adopting the serum - free and animal - source - free medium domestication express cell efficiently, setting up to express system efficiently, suspending culture cell, can raise the cell density in the scale turn the production, strengthen the cell vitality, control cell to propagate level, extension cell culture period, increase the target protein of yield, raise product quality, simplification of produces technics, reduce production cost, then raising the efficiency that the scale turns culture
提要：採用無血清無動物組分培養基馴化高效表達細胞，構建高效表達系統，懸浮培養細胞，可以在規模化生產中，提高細胞密度，增強細胞活力，控制細胞增殖水平，延長細胞培養周期，增加目標蛋白的產量，提高產品質量，簡化生產工藝，降低生產成本，進而提高規模化培養的效能。 -
Methods rabbit conjunctival epithelial tissues were explanted in serum - free system the morphology, gowth, proliferation and differentiation of outgrow cells were compared with those in serum - containing medium
方法分別用含胎牛血清培養液和不含血清培養液進行組織塊培養，觀察兩種培養方法在細胞形態、細胞生長情況和增殖及細胞分化方面的差異。 -
Thus, the success of nscs therapy is determined by the induced differentiation and the effect of micro - enviroment on the survival, proliferation and differentiation of it. the isolation and culture of nscs in vitro successfully is the presupposition for its research. to avoid the influence of unknown factors exiting in serum on nscs " differentiation, serum - free medium with egf and bfgf as mitogens to stimulate proliferation and inhibit differentiation in vitro was introduced through a long period of search
澱粉樣蛋白（ amyloidpeptidy ， a ）是澱粉樣蛋白前體蛋白（ amyloidprecusorprotein ， app ）異常代謝產生的含有39 ? 43個氨基酸殘基的疏水肽，可聚合成不溶的纖維形式或不定型顆粒狀結構，導致各種毒性反應，在阿爾茨海默病（ alzheimer ' sdisease ， ad ）發病中起著至關重要的作用。 -
Results the epithelial cells cultured in serum - free medium not only demonstrated a significant increase in proliferative, colony - forming efficiency and cell generations, but also have the normal structural and functional properties than those in serum - containing medium
結果無血清培養系統中結膜上皮細胞的增殖、克隆形成及細胞傳代能力均較含血清培養系統高，而且具備結膜上皮細胞全部組織結構及功能特點。 -
We report a novel three - stage method to efficiently direct the differentiation of human embryonic stem cells into hepatic cells in serum - free medium
我們報道一種新的三步法有效誘導人胚胎幹細胞在無血漿培養液中向肝細胞分化。 -
2. cytotoxicity assay hela, skov - 3, and wish cells were plated out in 96 - well plates at a density of 1. 0x104 cells per well in the serum - free rpmi - 1640 medium
值后發現和rl 』 a蛋白相比較， rta一yqrl細胞毒性作用明顯提高，分別是前者的2 ， 10和40倍。 -
Study on serum - free medium for 5b1 cell growth
1細胞生長的無血清培養基的研究 -
Medium which is serum - free or in the low concentration serum inhibit the proliferation of cells. a certain degree of increase serum concentration can promote the growth rate as well as prolong the life. the feeder layer cells were provided by mef, mue ( mouse uterus epithelium ) and rmc ( rat myocardial cells )
Mef和es細胞體外培養血清濃度以15 20為宜，試驗中以mef 、 mue 、 rmc作飼養層，結果表明：以12 . 5dpc的mef為飼養層，無論是在小鼠es細胞的原代培養，還是在克隆傳代過程中，效果都是最佳的。 -
To initiate induction, undifferentiated escs were dissociated and replanted on none - coated tissue culture plastic dishes in nsc medium. the medium was changed ever - y other day into serum - free nsc medium
誘導分化時去飼養層細胞，重新接種于無包被的培養皿中，使用nsc培養液，逐漸更換為無血清nsc培養液。 -
A successful cell strain for biopharmaceutical production must conform to the following characteristics : the cells could produce high level of recombinant proteins ; the cell should be adapted to serum - free or protein - free medium ; the cells should be resistant to adverse culture conditions, which means the cells should have some anti - apoptosis property ; if not cultured i n suspension, the cells should also be able to grow in adherence
一株成功的工程細胞除了要求目的蛋白的表達量高外，還必須適應無血清培養基培養；必須具有對不利環境的抵抗能力，即抗凋亡能力；對于非直接懸浮培養的細胞，還必須具備在無血清培養條件下的貼壁能力。 -
The stable expression cell line was selected. expression level of the line cultured in serum - free medium was 580iu 106 cell 24h. the product expressed was purified by zn
篩選到的穩定表達株在無血清培養基的表達量為580iu 106細胞24h 。 -
In my experiment, only beta - mercaptoethanol have the potency to induce mscs to neural cells, exclude the other antioxidant such as glutathione, ascorbic acid and vitamin e ( alcohol dissolved ), and using beta - mercaptoethanol and serum - free dmem medium as the main differentiation a - gent
結果顯示，只有beta -巰基乙醇具備此種誘導能力，排除了谷胱甘肽、抗壞血酸、維生素e等抗氧化劑的作用。本實驗使用了beta -巰基乙醇、無血清的dmem培養液和dmso作為誘導方案，在誘導后，可見很多誘導產生的神經元樣和膠質細胞樣細胞。 -
Materials and mehods nscs were isolated from e - 14 embryonic rat ' s brains, then cultured in serum - free medium with egf and bfgf ( 20ng / ml respectively ) as mitogens
12小時至36小時之間，死亡率上升最快: 36一48小時，死亡率幾乎不再上升，穩定在60 %左右。 -
However, the drawbacks of mammalian cell expression systems are apparent : they are difficult to culture in large scale, especially in serum - free medium ; the productivity is much lower than other expression systems and the costs are higher
但哺乳動物細胞表達系統的缺點也很明顯，如表達量低、大規模培養困難、生產成本高昂等。
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