shuttle vector 中文意思是什麼
shuttle vector
解釋
穿梭載體-
3. the effect of sporulation - independent promotor on toxicity of natural strain in order to study the effect of sporulation - independent promotor ( p3a ), p3a was spliced with the cry1c gene, then inserted into the shuttle vector pht304, and then recombinated plasmid pbmb827 was obtained. after transferring pbmb827 into strain ybt - 1520, it was surprising that the transformants had almost no potency against all lepidopteran larvae tested
3非依賴芽胞形成icp的cry3a啟動子（ p _ （ 3a ） ）對野生菌株特性的影響帶p _ （ 3a ）和cry1c基因的重組質粒pbmb827轉入ybt - 1520 ，轉化子對所測昆蟲的毒力下降非常明顯，芽胞和晶體也很難脫落。 -
In order to express alkaline protease gene ( ap gene ) in bacillus subtil is, the recombinant expression plasmid was constructed. this plasmid contains a promoter bp53, also from b. pumilus un - 31 - c - 42, ap gene and the shuttle vector psugv4. after introduced into b. subtilis wb600, the transformants displayed the hydrolyzed zone on milk plate
將來自短小芽抱桿菌un一31一c礴2的基因啟動子（ bp53片段）和脫毛蛋白酶全基因（ ap ）進行融合，然後將重組基因（命名為bpap ）插入到大腸桿菌-枯草桿菌穿梭質粒載體psugv4中，構建成表達質粒psu一bpap 。 -
In our laboratory, a unique mutation detection system using a shuttle vector plasmid has been established to demonstrate that a low concentration of mnng ( 0. 2 m ) can induce nontargeted mutation in mammalian cells : the mammalian cells were exposed to 0. 2m mnng for 2. 5h, then a shuttle plasmid pz189 carrying supf trna gene was transfected into cells after 24h culture. we found a 5 - fold higher mutation frequency of the plasmid replicated in pretreated cells than the spontaneous mutation frequency of the plasmid replicated in control cells. this kind of mutation did not occur immediately after mnng exposure
我們實驗室曾用一特殊的突變檢測系統，直接證明dna損傷劑可在哺乳動物細胞誘發非定標性突變：首先用低濃度（ 0 . 2 m ）的短壽烷化劑mnng （半壽期為1 . 1hr ）處理細胞2 . 5h后，繼續培養24h ，將重組有用作突變檢測的靶基因supftrna基因的穿梭質粒pz189轉入細胞復制，發現在未受致癌物直接攻擊的穿梭質粒中有較自發突變率高5倍以上的靶基因突變。 -
Bp23 celb genes, b. pwnilus endoglucanase and b. polymyxa beta - 1, 4 - endoglucanase " genes, respectively. it was recognized as a new gene encoding for endoglucanase of b. mega terium. the recombinant plasmid tvchi ( pmd18 ~ t inserted with chitinase encoding gene from aplls ) and e. coli - bacillus shuttle vector physooplk were digested by ecori and sail completely, and the chitinase gene was ligated with shuttle vector, and the recombinant vector was used to transform b. megaterium ap25 competent cell
平板拮抗實驗同野生菌株相比，轉化子對麥長蠕抱菌的抑制作用最明顯，抑制百分數最高可達33 . 3 % ，而apll3和ap25分別是23 . 1 %和25 . 6 % ，同時轉化子對小麥紋枯病菌、棉花立枯病菌、棉花枯萎病菌和小麥的全蝕病菌也具有較為明顯的抑制作用。 -
The expression plasmid called psugv - badfe was constructed by inserting ba - dfe gene into e. coli - b. subtilis shuttle vector psugv4 and the
將ba0fe基因克隆到大腸桿菌一枯草桿菌穿梭載體psugv4中，得到重組質粒psugv badfe ，然後轉化到枯草桿菌wb600中進行了分泌表達。 -
A bt - e. coli shuttle vector pht315 was deleted its replication region of bt, then constructed a novel vector named pht315 - 1 which composed a multiple cloning site, erythromycin and ampicillin - resistance marker and could only replicated in e. coli. used pht315 - 1, a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324. sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501, 333, 183aas. orfl had 98 % identities with replicating related protein ori43 of bt strain hd263. the others were no homology to any published bt replicating related protein. after continuous cultured for 70h at 30 c without antibiotic selecting press. the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 %. and growth curve also showed that the novel replicon was stable and could replicate normally
進一步序列分析表明該復制區至少有3個較大的orf ，分別編碼501 ， 333 ， 183個氨基酸。其中orf1蛋白序列與hd263復制蛋白ori43的同源性為98 ，而另外兩個orf和genbank己公布的bt復制相關蛋白無同源性。 30連續培養72h ，復制區質粒在bt無晶體突變株hd73cry ~ -中穩定性達98以上， 30h生長曲線也表明該復制區能夠在bt中穩定復制和遺傳，對受體菌株無明顯不良影響。 -
A 1. 5 kb apramycin resistance fragment was inserted into nru i site of aved gene and the inactivated aved gene fragment was then introduced into mcs region of phjl401 - an e. coli / streptomyces shuttle vector with conjugation function ( containing orit gene ). as a result of above procedures, a recombinant plasmid pid03 was obtained
將1 . 5kb的安普黴素抗性基因片段插入到aved基因中的nrui酶切位點，再將此滅活的aved基因片段插入到具有接合轉移功能（含有orit基因）的鏈黴菌?大腸桿菌穿梭質粒phjl401的多克隆位點區，由此得到重組質粒pid03 。 -
With the aim of increasing the expression and stability of mtxl, the mtxl gene originated from b. sphaericus ssii1 was cloned to a shuttle vector pbu4. two recombinant plasmids pmt9 and pmt4 were obtained, with the inserted fragments in the opposite orientations
為了提高mtx1殺蚊毒素蛋白的表達量和穩定性，本文將來源於球形芽孢桿菌b . sss - 1的mtx1毒素基因克隆至穿梭載體pbu4上，得到mtx1插入方向相反的重組質粒pmt9和pmt4 。 -
The sacvgb gene was then cloned into e. coli - - b. sublilis shuttle vector psugv4 and a recombinant plasmid named psu - sacvgb was constructed. after transformation with b. sublilis wb600, the transformants successfully produced vhb intracellularly. the influence of vhb on b. subtilis is under investigation
將sacvgb基因克隆到大腸桿菌-枯草桿菌穿梭載體psugv4中，獲得重組表達質粒psu - sacvgb ，然後轉化枯草桿菌wb600 ，轉化子經2蔗糖誘導，該基因在枯草桿菌中表達。 -
In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee
本實驗用pcr擴增hcv結構區基因，克隆到桿狀病毒表達載體pfastbacl中，構建成重組轉座載體pfb1 - cee ，轉化dh10bac大腸桿菌感受態細胞，篩選陽性菌落，抽提大分子質粒dna ，獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ，脂質體介導轉染sf9昆蟲細胞，出現細胞病變后，收集含有重組桿狀病毒顆粒的培養上消，重新感染sf9細胞，收集sf9細胞，進行12 . 5 sds -聚丙烯酰胺凝膠電泳，可見表達的蛋白條帶。 -
4. effect of resolution vector on the expression of pesticidal crystal protein genes this work successfully introduced pesticidal crystal protein genes into crystal negative strain bmb171 by using resolution shuttle vectors. after resolution, the expression of cry1ac and cry 1c genes have no obvious change, but the expression of cry3a genes has great increase in the same condition
解離反應對殺蟲晶體蛋白基因表達的影響成功地利用解離載體將crylac10 ， crylc和cry3a基因導入蘇雲金芽胞桿菌無晶體突變株， crylac和crylc基因解離前後的表達量和殺蟲毒力未見明顯變化， cry3a基因在相同條件下則表達量有所提高，至於為何只對基因cry3a有作用尚不清楚，國內外也未見有人作相關報道。 -
After its sequence was determined, the mutant gene was cloned into a shuttle vector pnov - r and transformed into br - deficient halobacteria ( l33 )
突變后的蛋白經生物信息學軟體分析了和野生型br的不同。 tyr79 argbr突變體的光電特性還要進一步分析和測試。 -
Therefore, the 4. 4kb fragment from pbl - 2 was ligated to a shuttle vector pht304 with bt replicon and transformed into acrystalliferous mutant 4d10 ( serotype hm, ) and resulted in a cloned strain bl - 3. this strain could express only 130kda protein
它不僅對蘇雲金芽胞桿菌工程菌的構建具有現實意義，而且對其它基因高表達研究將產生積極影響，具有一定的理論意義和潛在的應用價值 -
Cloning of soluble interleukin 4 receptor gene and construction of adenovirus shuttle vector
4受體基因克隆及腺病毒載體的構建 -
I ) two mutants ( hpab - 38 and hpab - 34 ) were designed based on the three - dimension structure of hpab - constructed by protein homology modeling method, ii ) the mutant molecules were generated by pcr and inserted into pfast hta donor plasmid, the later was then transformed into escherichia coli dhlobac to generate recombinant baculoviruses dna by site - specific transposition of an expression cassette into a baculovirus shuttle vector ( bacmid ) propagated in e. coli. the recombinant bacmids were isolated and transfected into insect sf - 9 cells to reproduce baculoviruses
4 . h隊b一p及其突變體高效表達工程菌的構建:將用pcr擴增的h隊b一p 、 h獄b一p35 、 h隊b一p34基因，分別與pinpointxa一3質粒連接，轉化jm109大腸埃希菌，獲得的工程菌在表達融合蛋白時不穩定，第5代后即見不到表達條帶。 -
Construction of corynebacterium glutamicum e. coli shuttle promoter - probe vector
大腸桿菌穿梭型啟動子探測載體構建
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