single-stranded dna 中文意思是什麼
single-stranded dna
解釋
單鏈、單股dna,單鏈脫氧核糖核酸,單股dna,單鏈dna-
Constructing cdna expressing library of erythrocytic plasmodium falciparum from hainan : the total rna was obtained by using. triplix kit. a modified oligo ( dt ) primer ( cds ffi pcr primer ) was used in the single - stranded ( ss ) dna synthesis reaction. the ss - dna was reversely transcripted from total rna. double - stranded ( ds ) cdna was amplified by long - distance ( ld ) pcrafter the digestion with proteinase k and sfi i, the cdna with no less than 200bp was collected and purified by glass - milk kitthe library was constructed after the ligation of cdna to tiplex2 phage particle packaged with the packaging extract system in vitro. a high titer and high recombinant ratio of cdna library was constructed
構建惡性瘧原蟲海南株紅內期cdna表達文庫提取紅內期惡性瘧原蟲海南株總rna ,直接以總rna為模板使用cdna文庫構建試劑盒,首先反轉錄合成ss一dna ,再擴增合成ds一dna ( cdna ) ,對擴增產物用蛋白酶k消化及左z丁i酶切,抽提蛋白、去除rna后,用玻璃奶試劑盒純化、回收20obp以上的片斷,經與載體連接再用蛋白包裝物包裝后形成未擴增文庫,最後擴增完成惡性瘧原蟲海南株紅內期cdna表達文庫的構建。 -
The orf1 proteins of all four chinese senv isolates had two motifs ( ftl and yxxk ) which were conserved in replication - associated proteins. in all four chinese senv isolates, the putative non - structure orf2 protein had a cav - like region. the putative orf3 protein had a serine - rich tract preceded by a cluster of basic amino acids ( r and k ). database scaning suggested this region had high a percentage of homology with dna topoisomerase i protein of d. melanogaster, and might play an important role in the replication of such single - stranded dna viruses
所有這四個中國分離株,均保留了o即1中與復制有關的保守序列( ftl和yxxk ) :在推測是非結構蛋白的orfz中均含有cav樣保守區w 『 x7一h一x3一c一xl一c一xs一h ; orf3蛋白的堿性氨基酸(精氨酸和賴氨酸)簇后緊接著一個富含絲氨酸的區域,與d . molanogaster的dna拓撲異構酶i有一定的同源性,推測在單鏈dna的復制中起作用。 -
Antisense nucleic acid technique is a method using single - stranded complementary sequences with specificity assigned by watson - crick base - pairing targeted specific messenger rna or dna to block expression of target gene
反義核酸技術是根據堿基互補原理,使用與目標靶的遺傳物質( mrna或dna )特定互補的核酸片段封閉基因表達的技術方法。 -
Dna single stranded breaks, ssbs, dna
單鏈斷裂
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