small g-protein 中文意思是什麼
small g-protein
解釋
小g蛋白-
Thioredoxins, an ubiquitous small proteins with a redox active disulfide bridge in its conserved motif - cp ( g ) pc -, are universally distributed in eucaryote and procaryote and have a molecular mass of approximately 12kda. by its disulfide / dithiol interchange reaction, this protein can transmit the regulatory signals to seleted targets ( enzymes, transcription factors etc ) and plays an important role in many plant physiological processes that includes photosynthesis, dna synthesis, transcription, protein disulfide reduction, protein repair, filamentous phage assembly, cell apoptosis and seeds germinating and so on
該蛋白質中含有保守的- cp （ g ） pc -氨基酸活性基序，該基序中的兩個半胱氨酸殘基可通過巰基二硫鍵的轉換實現其氧化還原狀態的變化和電子氫的傳遞，對細胞中與氧化還原相關的多種生理過程的調節起重要作用。通過同許多酶類、蛋白類、細胞內活性因子相藕連， trx能對光合作用、 dna復制、基因轉錄、細胞凋亡和生長、噬菌體組裝、蛋白質的還原和修復信號傳導等生理過程產生影響和調節。 -
Recent researches implicate that raplgap may interact with different type of alpha subunit in heterotrimeric g protein and suggest a cellular cross - talk between small g proteins and heterotrimeric g protein
最近研究發現，某些g亞單位可與rap1gap結合而調節rap1gap的活性或影響異源三聚體g蛋白的信號轉導。 -
In order to get small molecular g - protein rab3a, which serves to further investigate the interaction between rab3a and other proteins, we amplified the full coding region of rab3a cdna by polymerase chain reaction, using human placenta total cdna as template. the pcr products were recovered from gel electrophoresis and cloned into bamhi xhoi site of vector pyestrp2. the result of sequencing indicated that rab3a insertion fragment included its initiation and termination codons in 5 - and 3 - terminal, respectively
為了獲取全長的小分子g -蛋白rab3a ，以用於研究rab3a與其他蛋白相互作用關系，本實驗以人胎盤總cdna為模板， pcr擴增到人rab3a cdna全編碼區。產物回收后克隆于質粒pyestrp2的bamhi xhoi位點，測序結果表明，本實驗獲得的rab3a cdna包含了起始和終止密碼子。
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