solid phase antibody 中文意思是什麼
solid phase antibody
解釋
固相抗體-
2 - e4 - a and 82 - 6 are hybridized during their log growing time, and the hybrid - hybridomas are cloned for 3 times and produce 6 hybrid - hybridoma cells. the chromatosome of hybrid - hybridoma 3 - hu and hybridoma 2 - e4 - a and s2 - b are counted, and the antibody of ascites fluid or culture supernatant of 3 - hn is prepared. the positive clones are detected by three methods at the same time : rbc agglutination for monospecific anti - human rbc type a antibody, indirect elisa for anti - p24 antibody, and rbc solid - phase adherence for bispecific antibody
選其中一株3 - h _ ( 11 )做雜交-雜交瘤細胞染色體計數,同時計數兩母株2 - e _ 4 - a和s _ 2 - b的染色體數:制備腹水型和上清型抗體,用三種方法同時檢測其中的雙特異性抗體、單特異性抗人紅細胞抗體和抗p24抗體,即紅細胞固相吸附法測雙特異中文摘要性抗體,紅細胞凝集試驗測單特異性抗人a型紅細胞抗體,間接elisa法測抗p24抗體;用腹水型抗體做耐熱性及耐凍融實驗。 -
Methods : sandwich elisa assay was used, w6 / 32 mcab serving as solid phase antibody and 3 2m antibody as the first antibody. the second antibody - hrp conjugate was added for coloration. standard curve was obtained by shla - i standard reagent in serial dilution. the amount of shla - i in the samples was determined : 1
方法:以w6 32包被酶標板,捕捉樣品中可溶性hla ,加入一抗2m抗體,再加酶標二抗及底物顯色。根據可溶性hla -的不同濃度標準品顯色后的od值繪制標準曲線: 1
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