southern hybridization 中文意思是什麼

southern hybridization 解釋
dna雜交
  • southern : adj （superl southern most）1 南的，在南的；向南的；從南的。2 南方的，南部的，南國的。3 朝南的，...
  • hybridization : 混成作用
  1. It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity

    本研究中，木耳屬2個種的2個菌株在its區域表現出較高的保守性， 4種限制型內切酶的酶切圖譜沒有顯示出多態性；增加內切酶種類及供試菌株數量，有可能獲得具有多態性的限制性內切酶酶切圖譜； 9本實驗中， its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異，擴增片段長度均為650bp左右； 10根據形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析，紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法，兩者的關系是相輔相成，互為驗證
  2. Double cross - over strains aved24 and avec9 were obtained after growth of several generations on mym plate with or without antibiotics selection. genomic dna of double cross - over strains aved24 and avec9 were extracted and southern hybridization were performed

    分別提取同源雙交換茵株aved24和avec9基因組dna ，通過薩瑟恩（ southern ） dna印跡雜交，結果證明l
  3. The analysis results of southern blot hybridization among all of specimens are coincidence with the detecting results of pcr products electrophoresis

    所有標本進行southernblot雜交分析，結果予以證實。
  4. Fingerprints of 5 strains of the inbred mice and 2 strains of the inbred rats was conducted using a nonisotopically hrp labeled jl - 02 by the second institute of the public safety bureau of china and southern blot hybridization, the author studied many fingerprints of the same dna, the different organic fingerprints of the same organism and fingerprints of parent and offspring. the patterns were completely different among the different strains and those of the samples from the same strain were completely identical

    採用公安部二所自行研製的jl - 02多位點探針對5個品系的近交系小鼠和2個品系近交系大鼠進行了dna指紋分析，經過對同一dna的反復製作dna指紋圖和同一個體不同組織進行的dna指紋圖製作及對親代和子代（同品系內和不同品系間雜交）間的dna指紋圖比較。
  5. The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii. two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium. the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome. at the same time ; compared agrabactenum - mediated method with gene gun method, the transformation frequency of the former was 16. 7 %, while the latter was 50 %, so gene gun transformation method was suitable for long ya liiliwn

    用攜帶有幾丁質酶基因和- 1 、 3葡聚糖酶基因的工程菌，通過農桿菌介導法和基因槍轉化法轉化龍牙百合，經pcr和點雜交檢測證明外源基因已經整合到植物染色體中。同時對農桿菌介導法和基因槍法進行比較，發現農桿菌介導法的轉化率為16 . 7 ，基因槍法的轉化率為50 ，因此可能基因槍轉化法更適于龍牙百合的遺傳轉化。
  6. Hybridization bands were detected by southern blot analysis using lea3 gene probe labeled by digoxigenin the result showed that foreign lea3 gene integrated into strawberry genome

    2 。對點雜交為陽性的植株進行southernblot分析，轉化植株檢測到了雜交帶，除預期的1
  7. Rt - pcr combined southern hybridization analysis show that hap2 is expressed in leaves, tepals, and tepals, stamens of floral buds

    Rt pcr結合southern雜交分析表明， hapz在葉、花被片、再生花芽的花被片和雄蕊中均表達。
  8. As heterologous probe and subsequently show to code for desired enzymatic activity. after a serial of subcloning coupled with southern hybridization and enzymatic activity assay, the functional s. griseus atcc14811 cholesterol oxidase gene ( chog ) was localized onto 2. 3kb ecori - sall fragment

    對phz1140和phz1141進行bamh及bgl的酶譜分析及與choa探針的雜交，將膽同醇氧化酶基因初步定位在8 . 8kbbamh和9 . 9kbbgl片段上。
  9. I and hindlll respectively. after the digested products were run via agarose gel electrophoresis and transferred into nylon membrane, the southern blot was carried out using the cdna of rubber tree etrl as probe. the result of the southern blot showed that a hybridization band ( - 3. 0kb ) turned up from the ecor i digested product and another band ( - 4. 8kb ) turned up from the hindi ]

    從橡膠樹pr107品系的嫩葉提取基因組dna ，用限制性內切酶ecor 、 hinofll分別酶切，瓊脂糖凝膠電泳分離並轉膜后，用克隆的橡膠樹etri基因的cdna片段作探針進行southern雜交分析，結果表明， ecor酶切在約3 okb處有一條雜交帶出現， hi 。
  10. Pgxn217 was transferred into bradyrhizobium japonicum strain gx201 by triparental mating using the helper plasmid prk2073. marker exchange was achieved by selection on yma containing sm, km, gm, spc et al four kinds of antibiotics. mutants were confirmed by southern blotting and hybridization with the wild - type region and the tn5 fragment respectively

    隨后，利用突變質粒pgxn217誘變慢生型大豆根瘤菌菌株gx201 ，獲得了gx201的突變體菌株gx217 ；將pgxn201的3 . 4kbecori片段與plarf3連接獲得亞克隆pgxn201cl ，然後將pgxn201cl導入突變體菌株gx217 ，構建了gx217的功能互補菌株rgx217 。
  11. Strain sa - coo by southern hybridization. a cosmid - based gene library of streptomyces griseus atcc14811 was constructed using phz1357, a streptomyces - e. coli bifunctional vector carrying two cohesive sites

    為了獲得膽固醇氧化酶基因，以大腸桿菌-鏈黴菌雙功能柯斯質粒phz1357為載體，構建了灰色鏈黴菌atcc14811的基因組文庫。
  12. Methods : extracting the total rna of human pbmc, the objective include 60 healthy blood donator, 30 patient with viral encephalitis and multiple sclerosis and parkinsonian syndrome, 30 patient with schizophrenia and affective disorder, this indviuals were inpatients or outpatients of the first hospital of chongqing university of medical science from december, 2000 to june, 2001. using nested rt - pcr techique to detect borna disease virus " middle fragment in orf i, and using southern blot hybridization to analyze the pcr product

    重慶醫科大學碩士學位論文方法：提取從2000年12月至2001年6月在重慶醫科大學第一附屬醫院神經科和精神科住院及門診的60例健康獻血者、 30例包括原因未明的病毒性腦炎、多發性硬化、帕金森綜合征，以及30例包括精神分裂癥和情感障礙患者pbmc中的總kn 』 a ，採用套式逆轉錄聚合酶鏈反應estedrticr ）技術進行了bdvorf基因中部片段的檢測，並對pcr產物進行電泳分析及southernblot雜交分析。
  13. Integration through homologous recombination between the 2. 7 kb pks gene and the natural pks gene was confirmed by southern hybridization

    Southern雜交證實2 . 7kbpks基因和天然pks基因之間通過同源重組發生了整合。
  14. 1. expression of cry genes located in native plasmid in different flagella serotype strains to study cloning and expression of icps genes, an ecor i - f fragment of cryla ( a ) gene from pesi was inserted into pselect - 1 with t7 rna polymerase promoter in vitro. the plasmids of bacillus thuring fensisybt - 803 and ybt - 791 were analyzed by southern hybridization using an rna probe of ecori - f fragment and by pcr identification with cryl mixture primers

    將cry基因的高保守區的cry a （ a ） ecog - f片段插入帶有t7rna聚合酶啟動子的質粒pselect - 1 ，獲得了能在體外轉錄的rna探針載體pbpl - 1 ，用該載體制備的rna探針具有特異強，背景清楚，省時省力等優點，已成功地用於蘇雲金芽胞桿菌的分子生物學研究和特異菌株的篩選。
  15. Halobacillus trueperi accumulates glycine betaine under condition of high osmolarity. a partial fragment of the glycine betaine transporter beth gene was obtained from the genome of h, trueperi with degenerate primers. through southern blot hybridization and inverse pcr, a 5. 1 kb ecori fragment containing the beth gene was sequenced

    將擴增片段用地高辛標記成探針，與用不同限制性內切酶完全酶切的h . trueperi總dna片段作southern雜交，結果顯示在ecori酶切片段的5 . 1kb處有陽性信號。
  16. ( 3 ) because the expression signal of map2a was hard to be detected by the northern hybridization, we combined rt - pcr and southern hybridization to determine its expression

    （ 3 ）鑒于map2a基因的表達量較低，我們採用rt - pcr結合southern雜交的方法，研究了map2a基因在蘋果不同組織中的表達狀況。
  17. ( 2 ) it proved that there should be nucleotides sequence similar to mf - 14 in the sterile line by the southern hybridization using the fragment mf - 14 as probe

    （ 2 ）以回收得到特異片段mf - 14為探針，通過southern雜交檢測到白菜不育系中也含有該片段的同源序列。
  18. According to the southern hybridization results, 43 bacillus strains were divided into several groups, 22 strains were chosen randomly from all these groups for sequencing of aii gene. it was found that the nucleotide acid sequence of aii genes showed 85. 4 % - 100 % homology and amino acid sequence of aii proteins showed 8 8. 1 % - 100 % ho mology

    在southern雜交的基礎上，將檢測的43個菌株分組，對其中22個菌株的aii基因進行dna序列測定，結果表明，不同菌株aii基因的dna序列同源性為85 . 4 ? 100 ；而aii蛋白的氨基酸序列同源性為88 . 1 ? 100 。
  19. Fusion gene by pcr was inserted into bombyx mori baculovirus transfer vector pbacpak. 8 and contransfected with lineared dna of bm - bacpak6 virus into bmn cells. the homologous recombination occurred inside the cells, and the recombinant virus bacpak - 6aa - hgm - csf was expressed, as identified by pcr and southern hybridization. the bmn cells and the fifth instars were infected by the recombinant virus bacpak - 6aa - hgm - csf

    本研究首先通過pcr將家蠶桿狀病毒多角體蛋白起始密碼子后的18個堿基引入到hgm - csf基因的5 』端之前，然後將融合基因重組與家蠶桿狀病毒轉移載體pbacpak8中，獲得重組轉移載體pbacpak8 - 6aa - hgm - csf ，並與線性化bm - bacpak6dna共轉染家蠶細胞株，獲得重組病毒bacpak - 6aa - hgm - csf 。
  20. Dna fingerprints of 13 colonies of 5 strains, including 5 balb / c, 2 balb / c - nu / nu > 4 c51 1 cba / n and 2 dba / 2 from 7 factories of laboratory animal in the beijing and xi ' an areas, were studied with jl - 2 mulilocus probe and southern hybridization and the author compared the dna fingerprint with the biochemical marker enzyme method. it indicated that the fingerprint had 17 - 22 distinguishable fragments and these fragments had high polymorphisms. the fingerprints of balb / c and balb / c - nu / nu, which were abnormal at the hbb site with the biochemical marker enzyme method, was different from their normal groups

    實驗採用jl - 02多位點探針對北京和西安地區較大的7家實驗動物生產供應單位的5個balb c群， 2個balb c - nu nu群， 4個c _ （ 57 ）群， 1個cba n群和1個dba 2群近交系小鼠進行了dna指文圖分析，並與常規生化標記分析法進行了比較，結果顯示：所產生的dna指紋圖的圖帶數均在17 - 22條，具有良好的多態性。
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