specific binding 中文意思是什麼
specific binding
解釋
特異性結合-
Our previous studies showed existence of apoplast cam in the plant cell and cam had many extracellular functions. so it supposed cam may be one of important extracellular polypeptides and trigger the intracellular signal transduction by binding the receptor. in this study, radiolabelled ligand is used to investigate the binding characteristic of cam and a. thaliana protoplasts. and chemical crosslinking is employed to explore binding proteins in the membrane. at first, ( 35 ) ~ s - cam was produced using ( 35 ) ~ s - labeled amino acid mixture in e. coli. sds - page and autoradiograph indicated high - purified, high - specific radioactivity ( 35 ) ~ s - cam was obtained. electrophoresis of ( 35 ) ~ s - cam is the same as that of unlabeled cam with ca ~ ( 2 + ) or egta ; a quatitive of protoplasts was prepared by enzymolysis
首先，用~ （ 35 ） s標記的氨基酸混合物喂養工程菌成功地制備了~ （ 35 ） s標記的擬南芥鈣調素（ ~ （ 35 ） s - cam ） ， ~ （ 35 ） s - cam純度高、放射活度高、 ca ~ （ 2 + ）與egta存在時的電泳行為與未標記cam相同，可作為一種高靈敏性的探針用於進行受體學分析實驗；用擬南芥種子誘導愈傷，通過酶解制備了大量原生質體。 -
It focuses on the theory of the mineral functional materials " improving the anti - chloride permeability and the chloride binding. the main achievements of this article include : 1 ) use the technology of xrd, sem, bet, laser particle size distributor etc to test the component, the construction, particle size distributor, specific surface etc of micro - silica, ultra - fined slag, high quality fly ash, meta - kaolin clay, four types of the mineral functional material, theoretically analysis the functions which the four mineral functional minerals act on cement concrete, namely pozzolanicity effect, filling effect, micro - aggregate effect, surface adsorption effect and so bring into play the affect of plastification, lowing of temperature, reinforcement, improvement of durability. base on this analysis we can conclude that the proper diameter of the mineral functional material is 5 m - 15 m, that is blain fineness 4500cm2 / g - 7500cm2 / g
本論文的主要成果包括以下幾個方面： （ 1 ）採用xrd 、 sem 、 bet 、激光粒度分析儀等多種測試手段，探明了以硅灰，超細磨粒化高爐礦渣，優質粉煤灰及偏高嶺土超細粉等4種超細礦物功能材料的組成、結構、粒度分佈、比表面積等基本性質；從理論上分析了礦物功能材料在混凝土中的4種效應，即火山灰效應、填充密實效應、微集料效應、表面吸附效應，並由此發揮出增塑、溫峰削減（降熱） ，增強及耐久性改善等作用；通過相關理論分析，推導出礦物功能材料的合理粒徑范圍在5 m 15 m ，即勃氏比表面積為4500cm ~ 2 g 7500cm ~ 2 g 。 -
The global regulator csra of e. coli is a specific mrna - binding protein. csra negatively regulates several metabolic pathways that are induced post - exponentially, including glycogen biosynthesis, gluconeogenesis, and glycogen catabolism ; positively controls gene expression within the glycolytic pathway ; and also csra modulates the levels of enzymes that participate directly in pep metabolism
Csra是整體調控網路的調控基因，可負調控指數生長後期誘導的一些代謝途徑，包括糖原的生物合成、糖原的分解代謝、糖異生，而對糖酵解的一些重要基因的表達則執行正調控功能， csra也調控直接參與pep代謝的三個酶的活性水平。 -
Homologues of era have been identified in prokaryotes and eukaryotes. there are two domains in era : the n - terminal gtp - binding domain and c - terminal rna binding kh domain. era plays an important role in cell cycle progression at a specific point in the cycle, after chromosome partitioning but before cytokinesis
Era是在原核和真核生物以及植物中普遍存在的g蛋白，是一個結構上既具有gtp結合結構域又有rna結合結構域、不同於ras的獨特的新的g蛋白亞家族。 -
Specific binding is found in tissues where muscarinic receptors are demonstrable pharmacologically.
在藥理學方法證明有毒蕈堿樣受體的組織中發現了特異的結合作用。 -
Second, ca - np30 conjugate was made by binding ca with np30. then immunized balb / c mice with ca - np30, and evaluated its effects on protective immunity, serum specific igg antibody levels and delayed type hypersensitivity ( judged by footpad test )
將鈣納米顆粒ca與np30制備成ca皿30結合物（ ca np30 ） ，主動免疫b從b兒， j 、鼠，觀察其對， j 、鼠抗血吸蟲感染的保護作用、對血清特異性屹g抗體的影響和足墊反應。 -
The determination of human thymidine kinase ( htk ) in human serum, which is a key indicator of cancers can give information for the diagnosis and treatment of the malign diseases. the protein a layer was first self - assembled onto the gold electrode surfaces of quartz crystals, the monoclonal antibodies were then orientedly immobilized through the specific binding between the fc terminals of the antibodies and the self - assembled protein a. with this sensor, the affinity constant of antigen - antibody binding was estimated to be 1. 85 106 l / mol according to the scatchard ’ s plotting method, which proved the high bioactivity of antibody. finally, an amplified piezoelectric immunosensor was designed to determine the htk in
實驗中將蛋白a吸附於鍍金壓電石英晶體電極表面，用於定向固定htk單克隆抗體，成功研製了檢測htk的壓電石英晶體傳感器，並基於標準scatchard繪圖法，計算出免疫反應的親和常數為1 . 85 106l / mol ，證明該單克隆抗體具有較高的免疫活性;同時基於酶催化沉澱技術，設計了的檢測htk的質量放大壓電石英晶體傳感器，該傳感器可在0 . 1 - 10ng范圍內對htk進行定量檢測，應用此傳感器成功地對5種癌癥病人血清中htk的濃度進行了測定，實驗結果為癌癥的臨床診斷與治療提供了參考。 -
The env protein deduced from env gene encodes the hydrophilic surface protein ( su ) and the hydrophobic transmembrane domain ( tm ) that determine the specific interaction between virus particles and cell surface receptors during retroviral entry. the su of retroviruses is a highly variable genetic element, containing receptor binding sites and major antigenic determinants. exjsrv - specific dna probes were derived. by using these dna probes in tissue hybridization. we successfully identified jsrv mrna expression and proviruses dna in sheep lung tissues infected with jsrv and control group has no postive signals, validating the use of exogenous virus - specific dna probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences
用地高辛隨機引物法標記exjsrv特異的env片段，制備探針，原位雜交檢測spa肺組織中的rna及前病毒dna ，結果表明spa患羊肺組織內有jsrvenv基因mrna的表達，同時也檢測到了前病毒dna ，而相應的陰性對照卻無陽性信號，證實外源性病毒特異的dna探針在致瘤性前病毒的整合位點和整合的外源性前病毒的檢測中具有可信度。 -
Methods according to theory of specific binding of antigen and antibody, at first the anti - a monoclonal antibody ( ma ) and anti - bma were labeled with the fluorescent, then fluorescent - labeled antibodies ( fla ) were bound with corresponding biological material ( such as bloodstain ) in the optimum condition, finally the abo blood type of bloodstain was determined under microscope fluorescent
方法根據抗原抗體特異性結合的原理，首先對抗a 、抗b單克隆抗體進行熒光標記，然後使熒光標記抗體與相應抗原（血痕）在最佳條件下結合，最後熒光顯微鏡鏡檢，判定血痕的血型。 -
Estrogen through binding specific estrogen department of physiology 7 receptor ( er ) in nucleus, functions like a nucleus transcription factor and modulates expression of some genes. it has been found that there exist two subtypes of er. there are a and p subunits which are different in their distribution, density and mediated action
雌激素調節神經系統功能往往通過雌激素受體（ estrogenreceptor ， er ）蛋白介導。 er屬于類固醇受體超家族的核受體，具a 、 p兩種亞型，此二者在各種組織分佈及密度泅異。 -
The results of emsa showed the obvious retardant bands, which suggest that some proteins in nuclei can bind to the specific dna sequence of mbd3 gene after lps treatment. 5. the molecular mass of this dna binding protein was assessed between 43. 0 - 66. 2 kd by south - western blot
4 .電泳遷移率改變實驗有明顯的滯后帶形成，表明lps刺激后，肝臟組織的細胞核內有轉錄因子與mbd3基因特異的dna序列結合，參與mbd3基因的誘導表達5 . south一westemblot進一步確定此轉錄因子分子量在43 . 0一「 . 2kd之間。 -
Dendritic cells ( dc ) is the most powerful apc, which can markedly increase the antigen - presentation capacity by maximizing the pepitide - mhc complexes on the cell surface and upregulating the co - stimulatory ligands b7 - 1 and b7 - 2, adhesion moleculees such as il - 12 that promote full activation of lymphocytes. full activation of antigen - specific t cells requires two signals - one signal coming via the tcr and the other signal through engagment of co - stimulatary molecules. t cells receiving one signal via their tcr are turned off by mhc ( major histocompatibility complex ), via t cell cd28 binding to b7 on the dc induce tlymphokine and t cell proliferatiion
T細胞介導的細胞免疫在控制腫瘤生長方面發揮著重要作用， t細胞在發揮抗瘤效應（分泌細胞因子和直接殺傷）之前必須先經過活化，體內專職抗原提呈細胞（ apc ）細胞並使其活化，樹突狀細胞（ dendriticcell ， dc ）為t細胞的激活提供雙重信號， t細胞藉助tcr識別由dcmhc分子遞交的抗原肽后，通過tcr - cd3復合體傳遞抗原特異性識別信號（第一信號） ，以cd28為主的t細胞表面輔佐分子識別dc表面b7分子，傳遞非特異性協同刺激信號（第二信號） ，在機體抗腫瘤免疫應答中處于核心地位。 -
But mere is no specific provisions to following on how to implement the international legal duty. treaty application is a issue of national legal systems of each party. the wto agreements consisting of a series of agreements such as gatt, gats, trips, tprm, dus and so on, are binding upon all parties
「條約必須遵守」這一原則要求各締約方善意履行所共同承擔的條約義務，但該原則本身並未解決條約如何履行或者說國際條約在締約方國內法上如何適用的問題，這主要是一個國內法問題，取決于各國的法律傳統和憲法體制的不同。 -
The recombinant protein exhibited specific binding to antiviral antibodies of h5 subtype and has no cross - reaction with h7 and h9 subtype
分別用親和層析以及電洗脫兩種方法對gst - ha1融合蛋白進行了純化，都獲得了理想的純化結果。 -
Using a computer program, a peptide was deduced from residues 256 - 265 of human fas ligand, based on the hypothesis that it should contain a specific binding site to the corresponding fas. this short peptide and fas contain 70 % of sense - antisense pairs
本研究依據ahbs理論，應用自行編寫的軟體，在fasl分子中尋找到與fas相應的反義多肽最為接近的十肽結構即fasl _ （ 256 - 265 ） （匹配度為70 ） 。 -
Significant progress has been made not only in the basic science of generating specific binding molecules, but also in applications of the selected binders in laboratory procedures, proteomics, diagnostics and therapy
不僅在基礎研究方面取得的突破，還將其應用到實驗手段、蛋白組學、診斷學及疾病治療中。 -
These phage display peptides may be the tnfa mimotopes. part iii specific c7c tnfa mimotope phage clone as target for screening of tnfa specific binding - peptides from 12mer phage display peptide library : by using the specific c7c tnfa mimotope as target, we developed a novel approach for screening phage peptide library targeting specific phage display peptides. we used c7c tnfa mimotope phage clone lcs - 7 ( c - rrpaqsg - c ) as target to screen 12mer phage peptide library and identify the positive clones by phage elisa
3 、以特異性噬菌體克隆為靶篩選肽庫的研究? ?一種肽庫篩選新方法的建立：為了深入研究細胞因於與配基（受體或抗體）相互作用的關鍵殘基及其空間構象，在上述研究基礎上，我們以tnfa表位模擬肽克隆為靶篩選噬菌體十二肽庫，找尋與之結合的tnfa結合肽噬菌體克隆；並以此拓寬了噬菌體肽庫的應用范圍。 -
Also, you might want to adjust soap - specific binding properties
還有，您可能想調整特定於soap的綁定特性。 -
Specific binding information for every particular method is specified within
對于每個方法的特定綁定信息在 -
Antibodies are incubated with the solution, with later washing to remove non - specific binding
抗體溶液孵育，洗滌后清除非特異性結合
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