specific-pathogen free 中文意思是什麼
specific-pathogen free
解釋
無特殊病原體-
Two ndv strains, lasota and f48e9, were propagated in 9 - day - old embryonated specific - pathogen - free ( spf ) eggs respectively for 36 hours at 37, then these chicken embryos were placed at 4 for 12h before the allantoic fluid was gained
首先，將ndvlasota株和f48e9株分別接種於9日齡spf雞胚，於37孵化36h ，置4 12h后收獲雞胚尿囊液。 -
The nucleic acids of the all influenza viruses conducted in the research were extracted from the viruses propagated in specific - pathogen - free chicken embryos. all of the eight segments were amplified by rt - pcr, and the purified pcr products were done cycle sequencing with specific primers, furthermore, the sequencing products were purified and run gel on abi prism 377 dna sequencing machine. the specific primers of the eight genes for pcr and cycle sequencing were designed using the ohgo ( 4. 0 version ) and genedoc ( 2. 3 version ) software
首先將實驗用毒株在spf雞胚中增殖，提取核酸，然後應用oligo （ 4 . 0版本）和gendoc （ 2 . 3版本）軟體設計h9n2aiv所有8個基因片段特異的pcr引物及序列測定引物，通過rt - pcr方法擴增所有毒株的各個基因片段，純化後用特異引物進行測序反應，反應產物純化后在abiprism377dna序列分析儀上進行序列測定，然後應用wisconsinsequenceanalysispackage （ gcg ， 10 . 2版本） 、 phylogeneticanalysisusingparsimony （ paup ， 4 . 0版本）和treeview （ 1 . 5版本）軟體進行序列的數據編輯、序列翻譯、進化樹繪制和遺傳演化分析。 -
Establishment and administration of the specific pathogen free animal care facilities
級實驗動物房的建設及其管理
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