splicing site 中文意思是什麼

splicing site 解釋
剪接位點
  • splicing : （剪接）：指內含子切除和外顯子連接，因此內含子被剔除，而外顯子剪接到一起。
  • site : n 1 地點；位置；地基。2 場所，現場。3 遺址。4 【計算機】網站，站點〈電腦網路用戶的網站地址〉；萬...
  1. Genome - wide detection and analysis of alternative splicing for nucleotide binding site - leucine - rich repeats sequences in rice

    基因選擇性剪接的全基因組檢測及分析
  2. The mechanism is that the introduced complementary oligonucleotides can bind to the corresponding mrna or double - stranded dna in genome and form partial double - stranded molecules or triple - stranded nucleic acid molecules by sequence - specific and nonsequence - specific antisense action, thus the target gene will be orientationally blocked and expression of the target inhibited so that therapeutic effect could be attained. in this study, we designed a fragment of human c ii ta cdna in antisense orientation using mrna of c ii ta as template. the primers were designed based on 94 - 500 nucleotides segment in 5 " end of ciita gene so that the interested gene contained 407 base pairs which included two aug codons in 1 16 and 188 nucleotides as well as the splicing site between the first and the second exons

    本研究設計以c tamrna為模板的反義cdna片段，從c ta基因5 』端第94位到500位核苷酸段設計引物，目的片段407bp ，覆蓋第116和188位兩個aug密碼子，也包含了第一外顯子和第二外顯子間的剪接位點：用常規分子生物學方法構建了反義片段的腺病毒表達載體（ padeasy - 1系統） ；腺病毒載體經hek293細胞包裝產生含反義片段的重組腺病毒，用氯化銫密度梯度離心法獲得純化的高滴度腺病毒；進行體外基因轉移，分別用反義片段真核表達載體轉染p388d1細胞和用重組腺病毒感染hela細胞，觀察導入的c ta基因反義rna抑制細胞內組成型或誘導型c ta基因表達的作用，從而達到調控mhc -類分子表達的目的。
  3. One of these splicing signals sits at the beginning of the intron and is called the 5 ' ( five - prime ) splice site ; the others, located at the end of the intron, are known as the branch site, the polypyrimidine tract and, finally, the 3 ' ( three - prime ) splice site

    其中一段位在插入子的起頭，有一個剪接信號序列，標明5 '端剪接位；插入子的尾端有其他的信號：分支點、多嘧啶區，最後則是3 '端剪接位。
  4. A new method for the splicing - site recognition of rice dna sequences was designed. based on the gt - ag intron organization principal, support vector machines ( svm ) was used to predict the splicing sites. through machine learning, a model was built on some test data set of true and pseudo splicing sites

    根據真核生物內含子在剪切位點前後存在保守堿基的特徵，用支持向量機技術構建分類器模型，有效地在基因組序列中識別剪接位點， 3位點識別的準確度87 . 96 ，在5位點識別的準確度達85 . 41 。
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