staining method 中文意思是什麼

staining method 解釋
染色法
  • staining : 斑點染色
  • method : n 1 方法，方式；順序。2 （思想、言談上的）條理，規律，秩序。3 【生物學】分類法。4 〈M 〉【戲劇】...
  1. Dna specific staining ; namaur method ; chromosome structure ; allium cepa

    Dna特異染色nama - ur方法放射環結構洋蔥染色體
  2. Methods the diagnosis of chronic gastritis accords with updated sydney classification system, rapid urease test, histopathological staining method and ( superscript 13 ) c breath test were used in detection of hp

    方法慢性胃炎診斷標準參照最新悉尼分類系統，幽門螺桿菌檢測採用快速尿素酶試驗、病理組織學染色及（上標13 ） c呼氣試驗。
  3. Materials and methods the mouse, golden hamster and human sperm were incubated with endotoxin in different concentration for different time to get capacitation, respectively, and ar was induced by progesterone after capacitation, then the rates of capacitation and ar were detected by chlortetracycline ( ctc ) and hoechst 33258 fluorescent staining method. the medium was with endotoxin in different concentration in sperm - oocyte fusion step during ivf, then the fertilization rate was observed. the 1 - cell, 2 - cell and zona - free 2 - cell mouse embryos were incubated in the medium with endotoxin, then the rate of blastocysts was recorded

    方法取小鼠精子10份、金黃地鼠精子6份、人新鮮精液標本10份及人冷凍精液標本9份，分別與不同濃度內毒素共孵育進行體外獲能和孕酮誘導的頂體反應，應用金黴素和dna結合的熒光染料hoechest33258雙重熒光染色法檢測精子的獲能率和頂體反應率；小鼠體外受精實驗的精卵結合環節培養液中加入不同濃度的內毒素，觀察受精情況並記錄受精率；取小鼠1 -細胞胚胎、 2 -細胞胚胎和去卵透明帶2 -細胞胚胎，與不同濃度內毒素共孵育進行體外培養，觀察體外發育情況並記錄囊胚率。
  4. Section two the evaluation of biocompatibility of the acellular dermal matrix by the method of cell culture. the new born rat ' s epdermic cells were cultured with the acellular dermal matrix together as experiment group, while the epdermic cell were cultured simply as control. 24 hours later, under the invert microscope, the epidermic cells anchored well and transparent flat cells were observed in both groups. 7 days later, both cultured cells were taked out and fixed in 95 % ethanol, stained with hematoxylin and were observed under light microscope. many cleaved cells were observed in both groups. during cell culture, no pathogenic microganism was observed. so we considered the acellular dermal matrix was aseptic and had good biocompatibility. section three subdermal implantation of the acellular dermal matrix. 24 rats were used in the experiments. a piece of acellular dermal matrix ( 1. 5 x 1. 5cm2 ) was implanted beneath the dorsum skin flaps of each rat, 1 week, 2 weeks, 3 weeks and 4 weeks after implantation, 6 pieces of acellular dermal matrix were harvested and the size of implanted acellular dermal matrix were measured, the sections were used for he staining and observed under light microscope. the result were as folio wing : 1 - 2 weeks after implantation, the acellular dermal matrix began to adhere to the tissue around and turned red gradually ; 3 - 4weeks after implantation, the acellular dermal matrix adhered closely to the tissue around and could be recognized easily, 1 - 3 weeks after implantation, the size of implanted acellular dermal matrix had no statistical difference ( p > 0. 05 ). 4 weeks after implantation implanted acellular dermal matrix contracted ( p < 0. 05 ). under light microscope, l - 2weeks after implantation, the fibroblast cells infiltrated the acellular dermal matrix and a small amount endothelial cells of vessel and lympho - histiocytic cells infiltrated the acellular dermal matrix. 3 - 4 weeks after implantation, infiltrating blood vessels were evident. so we think that the acellular dermal matrix had low immunological reactions and could induce the infiltration of fibroblast macrophage cell and the endothelial cells of vessel

    結果如下：皮下包埋卜周者，無細胞真皮基質漸與周圍組織粘附，顏色由蒼白轉紅；皮下包埋3周者，無細胞真皮基質與周圍組織緊密枯附，盾晰葉辯；術后卜周，包埋的基質面積變化較包埋前無統計學差異o川0引，術后4周包埋的無細胞真皮基質面積較包埋前縮小j刃刀5 ） 。光鏡下術后卜周，宿主的淋巳組織細胞、成纖維細胞浸入生長，釉附在膠原纖維上，少量血管內皮細胞浸入基質；術后34周，無細胞真皮基質內較多的血管形成，故可認為無細胞真皮基質免疫原性低，能誘導宿主的成纖維細胞、巨噬細胞浸入生長，為一種新型的真皮替代物。第四部分無細胞真皮基質與自體斷層皮片復合移棺的研究， sd大鼠10隻，在其背部卜方造成全厚皮膚缺損的創面
  5. Gram staining method

    革蘭氏染色法
  6. The results showed that the number of polyhedra obtained by the staining method was less than that by hemacytometer, but the coefficient of variation of the samples was lower than that of hemacytometer method, thus the staining method can be used in quality control test of virus insecticides

    該染色計數法測得的多角體數量要低於血球計數板的計數結果，但變異系數較血球計數板計數法顯著較小，結果穩定，適用於病毒殺蟲劑的質量檢測。
  7. Using indirect immuno - fluorescein staining method, the localization of sp - 10, tmdc - iii and aeg on human, mouse, rabbit, pig, bull sperm was observed under confocomicroscopy. it was found that antigens sp - 10 and aeg express on acrosome of all kinds sperms, supporting their roles in sperm - egg fusion. it was firstly described that tmdc - iii express mostly on the equator, neck and some part of tail of human sperm, on the acrosome of mouse and bull sperms, on the acrosome and neck of pig sperm, on the head, neck, and middle piece, principal piece of tail of rabbit sperm

    利用間接免疫熒光法對aeg 、 sp - 10 、 tmdc -蛋白在人、小鼠、兔子、豬、牛精子上進行了精確定位， aeg 、 sp - 10在頂體部均有表達，確證了它們與精卵融合有關：首次對tmdc -蛋白在人、小鼠、兔子、豬、牛精子進行了精確定位，它主要存在於人精子尾部主段，小鼠和牛精子頂體，豬精子頂體和頸部，兔精子頭部、頸部和尾部的主段及尾段。
  8. Pap test is a simple and most effective method. it is painless and involves introducing a warmed speculum into the vagina and scraping some cells from the cervix, which are then placed on a slide, immersed in alcohol to preserve the cells and sent to the laboratory for staining and examination with the microscope

    做柏氏子宮頸塗片的方法很簡單，醫生?需要用一個小的刮匙，在女性的子宮頸部位挖取少量細胞，通過簡單的染色，病理科醫生便可以在顯微鏡下研究細胞的形態和病變。
  9. After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s

    通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。
  10. The present study, ( d by using immunohistochemical single, double or triple staining method, showed the expression, relationship and distribution pattern of fos - protein, gfap or th in rat cns, investigated the plastic response and relationship of rat lumbar spinal cord as and neurons to pain induced by the unilateral tibia and fibula fracture ; ( 2 ) by using a double immuno - electron - microscopic method, investigated the ultrastructural characters of junction areas between neurons and as in the dorsal horn of rat lumbar spinal cord following the unilateral tibia and fibula fracture ; ? after intrathecal application of the carbenoxolone, a gap junction blocker, recorded the paw withdrawal thermal latency and compared with control

    在腦干gfap - li星形膠質細胞主要分佈於mvz內的孤束核（ nts ） 、腹外側延髓（ vlm ）以第四軍醫大學博士學位論文及兩者之間的中間帶（ irt ）上。三叉神經脊束核尾側亞核（ vc ） 、外側楔柬核（ ecu ） 、藍斑（ lc ） 、臂旁外側核（ lp ） 、中縫大核（ rmg ） 、中腦導水管周圍灰質腹外側區（ vipag ） 、中縫背核（ dr ）等部位也出現一定數量的gfap陽性細胞。 f 。
  11. In this experiment three sperm proeins, sp - 10. aeg. tmdc - iii. that were cloned and expressed by brist u. k., immunized on rats for analysing their immunogenicity and the effects on the fertility. then using indirect immuno - fluorescein staining method, the localization of sp - 10, tmdc - iii and aeg on human, mouse, rabbit, pig, bull sperm was observed under confocomicroscopy. the results as follows : 1. the rats successfully produced antibody after immunization with antigens sp - 10, aeg, tmdc - iii respective

    本文用英國葛蘭素公司重組構建表達的三種抗原蛋白sp - io ， aeg ， tmdc -免疫大鼠，通過elisa實驗、免疫熒光組化實驗、交配實驗對它們的免疫原性、在體內的生殖作用、精子表達部位進行了分析，結果如下： 1
  12. Immunohistochemistry method was used to observe the temporal and spatial expression of nmdar2, signal molecules, skeleton proteins and connexins in son neurons and glias ( astrocytes and microglias ). radioimmunoassay was used to detect vasopressin ( vp ) content in plasma before and after hyperosmotic stimulation. ultrastructure between activated son astrocytes and neurons was observed by double immune - electron - microscopic staining method

    應用免疫組織化學方法光鏡下觀察高滲刺激后，大鼠視上核膠質細胞（星形膠質細胞和小膠質細胞）受體（ nmdar2 ） 、信號分子、骨架蛋白及縫隙連接蛋白的表達的時空變化；應用放免測定檢測高滲刺激前後血漿中vp含量。
  13. After transfecting the shrna based on telomerase htert into hela cells with calcium phosphate co - precipitation procedures, we detected hela cells viability by trpan blue staining method

    以磷酸鈣共沉澱轉染法將shrna轉染hela細胞。于不同的時間用臺盼藍染色法檢測細胞生存能力。
  14. The assay system of the biological activity of lymphotoxin was established using l929 cell as the sensitive target, lt international standard as the positive control and crystal violet staining method to detect viable cell after treated with lt. the best relationship between dosage and effect could be got if the cell seeding density in cell plate was 1. 6 0. 1 104 the dosage of amd was lug / ml, and the starting concentration of dilution in the plate of lt standard was 10 iu / ml with two fold dilution. the credibility of the established system was detected with rhtnfp developed by r & d

    為確定經上述步驟純化后得到的目的蛋自lt 27的生物活性，本研究以l929細胞為靶細胞、淋巴毒素國際標準品為參照，採用結晶紫染色法檢測經淋巴毒素處理后存活的細胞，對淋巴毒素生物活性測定的細胞接種濃度、淋巴毒素標準品板上稀釋的起始濃度和梯度稀釋的倍數、放線菌素d的使用劑量等進行條件實驗后，建立了人淋巴毒素生物活性測定方法。
  15. Tetrazole staining method for measuring seed vigor that is accurate and steady which is admited by international word. and that witch is easy for imaging processing

    四吐染色測定種子生活力是目前國際上承認的一種準確可靠的瀏定方法，且易於圖像處理，因此選取此方法作為種子活力的檢瀏方法。
  16. Objective to establish stable silver - staining method for showing nerve endings of muscle spindle in rats ' soleus muscle

    摘要目的建立快速穩定的肌梭內神經末梢的銀染方法。
  17. Methods an immunohistochemical staining method was used. results nk3 receptor - li was localized in somatic and dendritic profiles in the most parts and in neuropil in a few regions of the mouse central nervous system

    應用免疫組織化學染色方法對小鼠中樞系統內nk3受體的分佈進行了觀察，結果表明：在小鼠中樞神經系統的絕大部分區域， nk3受體樣免疫陽性產物位於胞體和樹突上，少部分區域位於神經氈（ neuropil ）內。
  18. Value of cd45 and pi staining method in identifying malignant from benign pleural effusion by dna analysis

    倍體分析在良惡性胸腔積液鑒別診斷中的意義
  19. Abstract : a practical staining method for counting polyhedra ( pibs ) in nucleopolyhedrovirus insecticide was developed

    文摘:本文介紹了一種直接檢測病毒殺蟲劑產品中多角體數量的染色計數方法。
  20. Thus the objective of this study is to find some ways to improve the efficiency of cell therapy, that is. to optimize the microenvironment of nscs and in turn prompt them to functinally repair abnormal cns. in the first part, optimization of x - gal staining method, which is correlated with ph, incubating time, perfusion and some other parameters, was successfully got and used in the subsequent experiment, viz, comparing the behaviours of primary nscs and immortal nsc line after transplantation

    鑒於此，為尋求各種方法提高移植效率，改善外源nscs存活及分化，進而促進其實現功能性替代，為臨床nscs治療提供理論基礎，本實驗進行了以下三部分的研究：實驗第一部分研究了以lacz作為移植細胞標志基因時x - gal染色的影響因素，並得出排除非特異背景的優化條件。
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