staphylococcal protein 中文意思是什麼
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The fusion protein was bactericidal active against staphylococcus aureus. in present study, we will truncate the none channel - forming do main, then attach the agrd to the pore - forming region ( k544 - i626 ) to construct a new engineered multidqmain protein machine - compact engineered peptide targeting staphylococcus aureus. such engineered peptide was constructed by linking the gene of staphylococcal agrd pheromone with the gene of c - terminal ( 1626 ) of colicin la pore - forming region ( k544 - i626 ) with site - directed mutation
利用點突變方法將金黃色葡萄球菌信息素agrd （ i型， ystcdfim ）的基因引入到大腸菌素fa梭基端1626基因上，並將限制性內切酶sacl酶切位點基因分別引入到大腸菌素fa的p4和k544上，通過酶切、膠回收、連接獲得含大腸菌素ia水性孔道結構域和金黃色葡萄球菌信息素agrd基因的重組質粒。 -
In the 1990s, the pheromones of gram - positive bacteria, which regulates the growth and toxin secretion of the same type bacteria, were identified they were peptides consisted by dozens of amino acids. the pheromones can auto - recognize membrane receptor of the identical types of bacteria. we had constructed a fusion protein named pheromonicin by introducing a staphylococcal pheromone agrd at the c - terminal of the colicin ia
丘小慶等利用信息素的自主導向特性和大腸菌素ia的致死性通道特性構建了由金黃色葡萄球菌信息素agrd和大腸菌素ia組成的融合蛋白，命名為pheromonicin ，該蛋白表現出了信息素和大腸菌素ia都不具有的抗金黃色葡萄球菌活性。 -
Strain bl21, and gene expression was induced by iptg. the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene. using ion exchange chromatography and gel filtration chromatography, the chimera proteins were purified, and both of them showed a single band in tricine - sds - page. the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities, but gain platelet aggregation inhibitory activities as well
通過離子交換層析和凝膠過濾層分別對兩種嵌合體蛋白進行純化，純化產物在tricine - sds - page中都顯示為單一條帶。活性分析結果表明兩種嵌合體蛋白在保留水蛭素抗凝血酶活力的同時，還呈現抗血小板聚集活性。 -
In conclusion, the compact engineered peptide can form lethal ion channel in the membrane of staphylococcus aureus guided by staphylococcal agrd pheromone, therefore the engineered peptide had bactericidal activity against staphylococcus aureus, especially against the drug - resistant strains. because of the properties mentioned above, the engineered peptide showed great potential in the future. the engineered peptide was an example of bactericidal protein machine combining two minidomains with different bioactivities and different protein origins
該工程多膚能在人工脂質膜上形成電壓依賴性離子通道，即當電壓由omv變為+ somv時通道立刻開放，電導驟然上升到某一數值，其後電導發生丫系列諧振式變化;而當電壓由+ somv變為一50mv時電導並不立即減小至o ，而出現正電壓狀態下類似的表現。
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