stepwise elution 中文意思是什麼
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Purification was conducted with ni - nta resin as there ' s a 6 x his tag at the n - terminal of the recombinant protein. after stepwise washing and elution at purification procedure, sublimated protein was obtained with the full - length ns5 accounting for more than 90 % and the aborted translated protein about 6. 5 %
用ni一nta樹脂親和純化表達產物，經過梯度漂洗和洗脫，獲得純度90 %以上的全長表達產物，另有6 . 5 %的非全長翻譯產物（約70kda ） 。
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